Abstract
Sulfur compounds are known to play crucial roles in plants facilitating tolerance to heavy metal stress. Previous studies have shown the protective role of exogenous cysteine (Cys) to improve chromium (Cr) tolerance. In the present study, we aimed to evaluate the capability of Cys (0.5 mM) to reduce chromium toxicity (100 μM) in rapeseed by physiological and proteomic analyses. Seedlings treated with Cr showed reduced growth and activities of antioxidant enzymes, and increased malondialdehyde (MDA) content. Exogenous application of Cys reduced Cr accumulation and MDA content, restored catalase (CAT) and peroxidase (POD) activities, and caused significant enhancements in seedling growth. 2-DE gel electrophoresis and mass spectrometry were performed to investigate changes in the root proteome in response to Cys and Cr. The Cr-induced down-regulations of glycine-rich RNA-binding protein, lactoylglutathione lyase, fructose-bisphosphate aldolase, and glutamine synthetase were restored with Cys. Additionally, Cys removed the Cr-induced up-regulation of some proteins including pathogenesis-related protein, major latex protein (MLP) like proteins, 22.0 kDa HSP, triosephosphate isomerase, glyceraldehyde-3-phosphate dehydrogenase, glutamine amidotransferase, cysteine synthase, and L-lactate dehydrogenase. Taken together, proteomic analysis revealed that exogenous Cys helps plants to overcome Cr stress by modulating energy metabolism, amino acid metabolism, and defense mechanism.
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Claims 9
Claim · model-written summary
Chromium reduced seedling growth and antioxidant enzyme activities.
- Taxon
- Brassica napus L.
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napust0 - Treatment
- Cre0
- Outcome
- growth and activities of antioxidant enzymese0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“Seedlings treated with Cr showed reduced growth and activities of antioxidant enzymes”
t0“Brassica napus L.”
Claim · model-written summary
Chromium increased MDA content.
- Taxon
- Brassica napus L.Brassica napust0
- Treatment
- Cre0
- Outcome
- malondialdehyde (MDA) contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Seedlings treated with Cr showed reduced growth and activities of antioxidant enzymes, and increased malondialdehyde (MDA) content.”
t0“Brassica napus L.”
Claim · model-written summary
Cysteine reduced chromium accumulation and MDA content.
- Taxon
- Brassica napus L.Brassica napust0
- Treatment
- Cyse0
- Outcome
- Cr accumulation and MDA contente0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous application of Cys reduced Cr accumulation and MDA content”
t0“Brassica napus L.”
Claim · model-written summary
Cysteine restored CAT and POD activities.
- Taxon
- Brassica napus L.Brassica napust0
- Treatment
- Cyse0
- Outcome
- catalase (CAT) and peroxidase (POD) activitiese0
- Direction
- restorede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous application of Cys reduced Cr accumulation and MDA content, restored catalase (CAT) and peroxidase (POD) activities”
t0“Brassica napus L.”
Claim · model-written summary
Cysteine significantly enhanced seedling growth.
- Taxon
- Brassica napus L.Brassica napust0
- Treatment
- Cyse0
- Outcome
- seedling growthe0
- Direction
- enhancementse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous application of Cys reduced Cr accumulation and MDA content, restored catalase (CAT) and peroxidase (POD) activities, and caused significant enhancements in seedling growth.”
t0“Brassica napus L.”
Claim · model-written summary
Cysteine restored chromium-downregulated glycine-rich RNA-binding protein, lactoylglutathione lyase and fructose-bisphosphate aldolase.
- Taxon
- Brassica napus L.Brassica napust0
- Treatment
- Cyse0
- Gene
- glycine-rich RNA-binding protein, lactoylglutathione lyase, fructose-bisphosphate aldolasee0
- Outcome
- down-regulationse0
- Direction
- restorede0
- Comparator
- Cr-inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Cr-induced down-regulations of glycine-rich RNA-binding protein, lactoylglutathione lyase, fructose-bisphosphate aldolase, and glutamine synthetase were restored with Cys.”
t0“Brassica napus L.”
Claim · model-written summary
Cysteine restored chromium-downregulated glutamine synthetase.
- Taxon
- Brassica napus L.Brassica napust0
- Treatment
- Cyse0
- Gene
- glutamine synthetasee0
- Outcome
- glutamine synthetasee0
- Direction
- restorede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“glutamine synthetase were restored with Cys.”
t0“Brassica napus L.”
Claim · model-written summary
Cysteine removed chromium-induced upregulation of pathogenesis-related and major latex proteins and HSP.
- Taxon
- Brassica napus L.Brassica napust0
- Treatment
- Cyse0
- Gene
- pathogenesis-related protein, major latex protein (MLP) like proteins, 22.0 kDa HSPe0
- Outcome
- up-regulatione0
- Direction
- removede0
- Comparator
- Cr-inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cys removed the Cr-induced up-regulation of some proteins including pathogenesis-related protein, major latex protein (MLP) like proteins, 22.0 kDa HSP”
t0“Brassica napus L.”
Claim · model-written summary
Cysteine removed chromium-induced upregulation of triosephosphate isomerase, GAPDH and glutamine amidotransferase.
- Taxon
- Brassica napus L.Brassica napust0
- Treatment
- Cyse0
- Gene
- triosephosphate isomerase, glyceraldehyde-3-phosphate dehydrogenase, glutamine amidotransferasee0
- Outcome
- up-regulatione0
- Direction
- removede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cys removed the Cr-induced up-regulation of some proteins including pathogenesis-related protein, major latex protein (MLP) like proteins, 22.0 kDa HSP, triosephosphate isomerase, glyceraldehyde-3-phosphate dehydrogenase, glutamine amidotransferase”
t0“Brassica napus L.”
The same questions in other papers
chromium atom · physiology: antioxidant enzymes · Brassica napus (oilseed rape)Results agree
1 paper in the same direction as this paper
- negative
chromium atom · physiology: oxidative · Brassica napus (oilseed rape)Results agree
4 papers in the same direction as this paper
- negative
- negative
- negative
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.