Abstract
Abstract WRKYs play important roles in plant growth, defense regulation, and the stress response. However, the mechanisms through which WRKYs are involved in drought tolerance have been rarely characterized in kale (Brassica oleracea var. acephala DC). In this study, we cloned the BoWRKY10 gene from kale and its expression was induced with PEG4000, NaCl, gibberellic acid, cold, H2O2, and abscisic acid (ABA). Bo-WRKY10 was localized in the nucleus. The protein had a WRKY domain and a C2H2 zinc finger structure and belonged to subgroup II. The analysis of yeast transcriptional activity showed that BoWRKY10 may have transcriptional activation activity, which was mainly determined by the carboxyl terminal sequence. BoW-RKY10-overexpressing tobacco (Nicotiana tabacum L.) showed enhanced drought tolerance. After the drought treatment, relative water content and proline contents as well as superoxide dismutase activity were higher in transgenic plants, while malondialdehyde and H2O2 contents were lower. In addition, several genes related to the ABA signaling pathway, sucrose, and the reactive oxygen species scavenging system, were significantly upregulated in the transgenic lines. These results demonstrate that BoWRKY10 confers drought tolerance in tobacco. These results provide clues regarding the mechanism by which BoWRKY10 contributes to the regulation of drought stress tolerance.
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Claims 9
Claim · model-written summary
BoWRKY10 was cloned from kale.
- Taxon
- kale
Brassica oleracea var. viridise0 - Gene
- BoWRKY10e0
- Outcome
- clonede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we cloned the BoWRKY10 gene from kale”
Claim · model-written summary
PEG4000, NaCl, gibberellic acid, cold, H2O2, and ABA induced BoWRKY10 expression in kale.
- Taxon
- kaleBrassica oleracea var. viridist0
- Treatment
- PEG4000, NaCl, gibberellic acid, cold, H2O2, and abscisic acid (ABA)e0
- Gene
- BoWRKY10t0
- Outcome
- expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its expression was induced with PEG4000, NaCl, gibberellic acid, cold, H2O2, and abscisic acid (ABA)”
t0“Kale WRKY Gene BoWRKY10”
Claim · model-written summary
Bo-WRKY10 localized in the nucleus.
- Taxon
- kaleBrassica oleracea var. viridist0
- Gene
- Bo-WRKY10e0
- Outcome
- localized in the nucleuse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Bo-WRKY10 was localized in the nucleus”
t0“Kale”
Claim · model-written summary
Bo-WRKY10 had a WRKY domain and C2H2 zinc finger structure and belonged to subgroup II.
- Taxon
- kaleBrassica oleracea var. viridist0
- Gene
- Bo-WRKY10t1
- Outcome
- WRKY domain and a C2H2 zinc finger structure and belonged to subgroup IIe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The protein had a WRKY domain and a C2H2 zinc finger structure and belonged to subgroup II”
t0“Kale”
t1“Bo-WRKY10 was localized in the nucleus”
Claim · model-written summary
BoWRKY10 may have transcriptional activation activity, mainly determined by its carboxyl terminal sequence.
- Taxon
- kaleBrassica oleracea var. viridist0
- Gene
- BoWRKY10e0
- Outcome
- transcriptional activation activitye0
- Qualifier
- carboxyl terminal sequencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BoWRKY10 may have transcriptional activation activity, which was mainly determined by the carboxyl terminal sequence”
t0“Kale”
Claim · model-written summary
Bo-WRKY10 overexpression enhanced drought tolerance in tobacco.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- BoW-RKY10-overexpressinge0
- Gene
- BoW-RKY10e0
- Outcome
- drought tolerancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BoW-RKY10-overexpressing tobacco (Nicotiana tabacum L.) showed enhanced drought tolerance”
Claim · model-written summary
After drought, transgenic tobacco had higher relative water content, proline contents, and superoxide dismutase activity.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- transgenic plantse0
- Outcome
- relative water content and proline contents as well as superoxide dismutase activitye0
- Direction
- highere0
- Qualifier
- After the drought treatmente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After the drought treatment, relative water content and proline contents as well as superoxide dismutase activity were higher in transgenic plants”
t0“Tobacco”
Claim · model-written summary
After drought, transgenic tobacco had lower malondialdehyde and H2O2 contents.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- transgenict0
- Outcome
- malondialdehyde and H2O2 contentse0
- Direction
- lowere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“malondialdehyde and H2O2 contents were lower”
t0“Transgenic Tobacco”
Claim · model-written summary
Genes related to ABA signaling, sucrose, and reactive oxygen species scavenging were significantly upregulated in transgenic tobacco.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- transgenic linese0
- Outcome
- genes related to the ABA signaling pathway, sucrose, and the reactive oxygen species scavenging systeme0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“several genes related to the ABA signaling pathway, sucrose, and the reactive oxygen species scavenging system, were significantly upregulated in the transgenic lines”
t0“Tobacco”
The same questions in other papers
(+)-abscisic acid · molecular: expression · Brassica oleracea (wild cabbage)Positive and negative results
1 in the opposite direction
- no effect
Gibberellic acid · molecular: expression · Brassica oleracea (wild cabbage)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
hydrogen peroxide · molecular: expression · Brassica oleracea (wild cabbage)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
drought · molecular: expression · Brassica oleracea (wild cabbage)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
cold · molecular: expression · Brassica oleracea (wild cabbage)Positive and negative results
4 papers in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.