Abstract
A near isogenic line (NIL) of Brassica oleracea var. botrytis with resistant and susceptible lines C712 and C731, was used in this study. More than 100 differentially expressed cDNA fragments were obtained from black rot resistant cauliflower plants obtained using cDNA-amplified fragment length polymorphism (AFLP) after infection with the pathogen. Thirteen of these fragments were cloned and subjected to reverse Northern blot analysis using both infected and control cDNA pools. Two positive clones, M2 and M6, were isolated. Northern dot blot and Northern blot analyses showed that M2 was constitutively expressed, whereas M6 contained a gene that was differentially expressed during pathogen infection. Moreover, M6 cDNA fragment was also highly expressed 16-24 h after H2O2 treatment. Southern blots showed that M6 is a single copy gene in the cauliflower genome, and encodes a protein with 84 % homology to gene on Arabidopsis chromosome 1. The deduced M6 protein has 91 % positive homology with the Arabidopsis 2A6 protein, which regulates ethylene synthesis; 76 % homology with a 1-aminocyclopropane-1-carboxylate oxidase (ACO), the last enzyme in ethylene synthesis; and 70 % homology with an ethylene induced DNA binding factor. These results suggest that M6 gene fragment is a new H2O2 downstream defense related gene fragment and can be induced by Xanthomonas campestris pv. campestris and H2O2.
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Claims 9
Claim · model-written summary
M6 was differentially expressed in cauliflower during Xanthomonas campestris infection.
- Taxon
- Brassica oleracea var. botrytis
Brassica oleracea var. botrytise0 - Interactor
- Xanthomonas campestris
_(25868012152).jpg?width=60)
Xanthomonas campestrist0pathogen - Gene
- M6e2
- Outcome
- differentially expressede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_(25868012152).jpg?width=500)
Verbatim
e0“A near isogenic line (NIL) of Brassica oleracea var. botrytis with resistant and susceptible lines C712 and C731”
e2“M6 contained a gene that was differentially expressed during pathogen infection”
t0“after Xanthomonas campestris inoculation”
Claim · model-written summary
M6 was highly expressed 16-24 h after H2O2 treatment.
- Taxon
- Brassica oleracea var. botrytisBrassica oleracea var. botrytise0
- Treatment
- H2O2 treatmente1
- Gene
- M6e1
- Outcome
- highly expressede1
- Direction
- increaseinferrede1
- Qualifier
- 16-24 h aftere1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A near isogenic line (NIL) of Brassica oleracea var. botrytis with resistant and susceptible lines C712 and C731”
e1“M6 cDNA fragment was also highly expressed 16-24 h after H2O2 treatment”
Claim · model-written summary
M2 was constitutively expressed in cauliflower.
- Taxon
- Brassica oleracea var. botrytisBrassica oleracea var. botrytise0
- Gene
- M2e4
- Outcome
- constitutively expressede4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A near isogenic line (NIL) of Brassica oleracea var. botrytis with resistant and susceptible lines C712 and C731”
e4“M2 was constitutively expressed”
Claim · model-written summary
M6 is a single copy gene in the cauliflower genome.
- Taxon
- cauliflowerBrassica oleracea var. botrytise3
- Gene
- M6e3
- Outcome
- single copy genee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“M6 is a single copy gene in the cauliflower genome”
Claim · model-written summary
The M6 protein shares 84% homology with a gene on Arabidopsis chromosome 1.
- Taxon
- Brassica oleracea var. botrytisBrassica oleracea var. botrytise0
- Gene
- M6e3
- Outcome
- homologye6
- Value
- 84 %e6
- Qualifier
- to gene on Arabidopsis chromosome 1e6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A near isogenic line (NIL) of Brassica oleracea var. botrytis with resistant and susceptible lines C712 and C731”
e3“M6 is a single copy gene in the cauliflower genome”
e6“encodes a protein with 84 % homology to gene on Arabidopsis chromosome 1”
Claim · model-written summary
The deduced M6 protein has 91% positive homology with the Arabidopsis 2A6 protein, which regulates ethylene synthesis.
- Taxon
- Brassica oleracea var. botrytisBrassica oleracea var. botrytise0
- Gene
- M6 proteine7
- Outcome
- positive homologye7
- Value
- 91 %e7
- Qualifier
- regulates ethylene synthesise7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A near isogenic line (NIL) of Brassica oleracea var. botrytis with resistant and susceptible lines C712 and C731”
e7“The deduced M6 protein has 91 % positive homology with the Arabidopsis 2A6 protein, which regulates ethylene synthesis”
Claim · model-written summary
The M6 protein shares 76% homology with 1-aminocyclopropane-1-carboxylate oxidase (ACO), the last enzyme in ethylene synthesis.
- Taxon
- Brassica oleracea var. botrytisBrassica oleracea var. botrytise0
- Gene
- M6e3
- Outcome
- homologye1
- Value
- 76 %e1
- Qualifier
- 1-aminocyclopropane-1-carboxylate oxidase (ACO)e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A near isogenic line (NIL) of Brassica oleracea var. botrytis with resistant and susceptible lines C712 and C731”
e3“M6 is a single copy gene in the cauliflower genome”
e1“76 % homology with a 1-aminocyclopropane-1-carboxylate oxidase (ACO)”
Claim · model-written summary
The M6 protein shares 70% homology with an ethylene induced DNA binding factor.
- Taxon
- Brassica oleracea var. botrytisBrassica oleracea var. botrytise0
- Gene
- M6e3
- Outcome
- homologye1
- Value
- 70 %e1
- Qualifier
- an ethylene induced DNA binding factore1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A near isogenic line (NIL) of Brassica oleracea var. botrytis with resistant and susceptible lines C712 and C731”
e3“M6 is a single copy gene in the cauliflower genome”
e1“70 % homology with an ethylene induced DNA binding factor”
Claim · model-written summary
The M6 gene fragment is a H2O2 downstream defense related gene fragment that can be induced by Xanthomonas campestris pv. campestris and H2O2.
- Taxon
- Brassica oleracea var. botrytisBrassica oleracea var. botrytise0
- Treatment
- H2O2e5
- Interactor
- Xanthomonas campestris pv. campestris
Xanthomonas campestris pv. campestrise5pathogen - Gene
- M6 gene fragmente5
- Outcome
- defense related gene fragmente5
- Qualifier
- can be induced by Xanthomonas campestris pv. campestris and H2O2e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A near isogenic line (NIL) of Brassica oleracea var. botrytis with resistant and susceptible lines C712 and C731”
e5“These results suggest that M6 gene fragment is a new H2O2 downstream defense related gene fragment and can be induced by Xanthomonas campestris pv. campestris and H2O2”
The same questions in other papers
hydrogen peroxide · molecular: expression · Brassica oleracea (wild cabbage)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.