Abstract
Flavonols and other phenylpropanoids protect plants from biotic and abiotic stress and are dietarily desirable because of their health-promoting properties. The ability to develop new potatoes (Solanum tuberosum) with optimal types and amounts of phenylpropanoids is limited by lack of knowledge about the regulatory mechanisms. Exogenous sucrose increased flavonols, whereas overexpression of the MYB StAN1 induced sucrolytic gene expression. Heterologous StAN1 protein bound promoter fragments from sucrolytic genes (SUSY1 and INV1). Two additional MYBs and one microRNA were identified that regulated potato flavonols. Overexpression analysis showed MYB12A and C increased amounts of flavonols and other phenylpropanoids. Endogenous flavonol amounts in light-exposed organs were much higher those in the dark. Expression levels of StMYB12A and C were high in flowers but low in tubers. Transient overexpression of miR858 altered potato flavonol metabolism. Endogenous StmiR858 expression was much lower in flowers than leaves and correlated with flavonol amounts in these organs. Collectively, these findings support the hypothesis that sucrose, MYBs, and miRNA control potato phenylpropanoid metabolism in a finely tuned manner that includes a feedback loop between sucrose and StAN1. These findings will aid in the development of potatoes with phenylpropanoid profiles optimized for crop performance and human health.
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Claims 10
Claim · model-written summary
Exogenous sucrose increased potato flavonols.
- Taxon
- potato
Solanum tuberosumt0 - Treatment
- Exogenous sucrosee1
- Outcome
- flavonolse1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“mediate potato flavonol biosynthesis”
e1“Exogenous sucrose increased flavonols”
Claim · model-written summary
Overexpression of the MYB StAN1 induced sucrolytic gene expression in potato.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- overexpression of the MYB StAN1e2
- Gene
- StAN1e2
- Outcome
- sucrolytic gene expressione2
- Direction
- inducede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“mediate potato flavonol biosynthesis”
e2“overexpression of the MYB StAN1 induced sucrolytic gene expression”
Claim · model-written summary
Heterologous StAN1 protein bound promoter fragments from sucrolytic genes (SUSY1 and INV1).
- Taxon
- potatoSolanum tuberosumt0
- Gene
- StAN1e3
- Outcome
- bound promoter fragmentse3
- Qualifier
- from sucrolytic genes (SUSY1 and INV1)e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“mediate potato flavonol biosynthesis”
e3“Heterologous StAN1 protein bound promoter fragments from sucrolytic genes (SUSY1 and INV1)”
Claim · model-written summary
Two additional MYBs and one microRNA were identified that regulated potato flavonols.
- Taxon
- potatoSolanum tuberosumt0
- Outcome
- potato flavonolse4
- Qualifier
- Two additional MYBs and one microRNAe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“mediate potato flavonol biosynthesis”
e4“Two additional MYBs and one microRNA were identified that regulated potato flavonols”
Claim · model-written summary
Overexpression of MYB12A and C increased flavonols and other phenylpropanoids in potato.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- Overexpression analysise5
- Gene
- MYB12A and Ce5
- Outcome
- amounts of flavonols and other phenylpropanoidse5
- Direction
- increasede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“mediate potato flavonol biosynthesis”
e5“Overexpression analysis showed MYB12A and C increased amounts of flavonols and other phenylpropanoids”
Claim · model-written summary
StMYB12A and C expression was high in potato flowers but low in tubers.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- flowerse6
- Gene
- StMYB12A and Ce6
- Outcome
- Expression levels of StMYB12A and Ce6
- Direction
- highe6
- Qualifier
- low in tuberse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“mediate potato flavonol biosynthesis”
e6“Expression levels of StMYB12A and C were high in flowers but low in tubers”
Claim · model-written summary
Endogenous flavonol amounts in light-exposed potato organs were much higher than in the dark.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- light-exposed organse7
- Outcome
- Endogenous flavonol amountse7
- Direction
- much highere7
- Comparator
- the darke7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“mediate potato flavonol biosynthesis”
e7“Endogenous flavonol amounts in light-exposed organs were much higher those in the dark”
Claim · model-written summary
Endogenous StmiR858 expression was much lower in potato flowers than in leaves.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- flowerse7
- Gene
- StmiR858e7
- Outcome
- Endogenous StmiR858 expressione7
- Direction
- much lowere7
- Comparator
- leavese7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“mediate potato flavonol biosynthesis”
e7“Endogenous StmiR858 expression was much lower in flowers than leaves and correlated with flavonol amounts in these organs”
Claim · model-written summary
StmiR858 expression correlated with flavonol amounts in potato organs.
- Taxon
- potatoSolanum tuberosumt0
- Gene
- StmiR858e7
- Outcome
- flavonol amountse7
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“mediate potato flavonol biosynthesis”
e7“Endogenous StmiR858 expression was much lower in flowers than leaves and correlated with flavonol amounts in these organs”
Claim · model-written summary
Transient overexpression of miR858 altered potato flavonol metabolism.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- Transient overexpression of miR858e1
- Gene
- miR858e1
- Outcome
- potato flavonol metabolisme1
- Direction
- alterede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“mediate potato flavonol biosynthesis”
e1“Transient overexpression of miR858 altered potato flavonol metabolism”
The same questions in other papers
sucrose · molecular: metabolites · Solanum tuberosum (potato)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.