Abstract
Rhizoctonia solani is a necrotrophic fungal pathogen that causes disease in diverse plant species. In recent years, the genomic and transcriptomic studies have identified several candidate pathogenicity determinants of R. solani; however, most of them remain to be validated. In this study, we report a viral vector-based host-induced gene silencing (HIGS) as well as a dsRNA (double-stranded RNA)-based approach to effectively downregulate genes of R. solani AG1-IA (BRS1 strain) during pathogenesis in tomato. We tested a few of the in-planta upregulated R. solani genes and observed that silencing of one of them, i.e., RS_CRZ1 (a C2H2 type zinc finger transcription factor) significantly compromises the pathogenesis of R. solani in tomato. The RS_CRZ1-silenced plants not only exhibited significant reduction in disease symptoms, but the depth of pathogen colonization was also compromised. Furthermore, we identified the R. solani genes that were coregulated with RS_CRZ1 during the pathogenicity process. The HIGS-mediated silencing of a few of them [CL1756Contig1; subtilisin-like protease and CL1817Contig2; 2OG-Fe(II) oxygenase] compromised the pathogenesis of R. solani in tomato. The ectopic expression of RS_CRZ1 complemented the crz1 mutant of yeast and restored tolerance against various metal ion stress. Overall, our study reveals the importance of RS_CRZ1 in managing the hostile environment encountered during host colonization. Also, it emphasizes the relevance of the HIGS and dsRNA-based gene silencing approach toward functional characterization of pathogenicity determinants of R. solani. [Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY 4.0 International license .
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Claims 7
Claim · model-written summary
Silencing RS_CRZ1 reduced pathogenesis of R. solani in tomato.
- Taxon
- Tomato
Solanum lycopersicumt0 - Treatment
- silencinge0
- Interactor
- R. solani
Rhizoctonia solanie0pathogen - Gene
- RS_CRZ1e0
- Outcome
- pathogenesis of R. solanie0
- Direction
- compromisese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We tested a few of the in-planta upregulated R. solani genes and observed that silencing of one of them, i.e., RS_CRZ1 (a C2H2 type zinc finger transcription factor) significantly compromises the pathogenesis of R. solani in tomato.”
t0“Tomato”
Claim · model-written summary
Silencing RS_CRZ1 reduced disease symptoms in tomato.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- silencinge1
- Interactor
- R. solaniRhizoctonia solanie1pathogene0
- Gene
- RS_CRZ1e0
- Outcome
- disease symptomse0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The RS_CRZ1-silenced plants not only exhibited significant reduction in disease symptoms, but the depth of pathogen colonization was also compromised.”
t0“Tomato”
e1“We tested a few of the in-planta upregulated R. solani genes and observed that silencing of one of them, i.e., RS_CRZ1 (a C2H2 type zinc finger transcription factor) significantly compromises the pathogenesis of R. solani in tomato.”
Claim · model-written summary
Silencing RS_CRZ1 reduced depth of pathogen colonization in tomato.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- silencinge1
- Interactor
- R. solaniRhizoctonia solanie1pathogene0
- Gene
- RS_CRZ1e0
- Outcome
- depth of pathogen colonizatione0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The RS_CRZ1-silenced plants not only exhibited significant reduction in disease symptoms, but the depth of pathogen colonization was also compromised.”
t0“Tomato”
e1“We tested a few of the in-planta upregulated R. solani genes and observed that silencing of one of them, i.e., RS_CRZ1 (a C2H2 type zinc finger transcription factor) significantly compromises the pathogenesis of R. solani in tomato.”
Claim · model-written summary
Silencing CL1756Contig1 reduced pathogenesis of R. solani in tomato.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- silencinge0
- Interactor
- R. solaniRhizoctonia solanie0pathogen
- Gene
- CL1756Contig1e0
- Outcome
- pathogenesis of R. solanie0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The HIGS-mediated silencing of a few of them [CL1756Contig1; subtilisin-like protease and CL1817Contig2; 2OG-Fe(II) oxygenase] compromised the pathogenesis of R. solani in tomato.”
t0“Tomato”
Claim · model-written summary
Silencing CL1817Contig2 reduced pathogenesis of R. solani in tomato.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- silencinge0
- Interactor
- R. solaniRhizoctonia solanie0pathogen
- Gene
- CL1817Contig2e0
- Outcome
- pathogenesis of R. solanie0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The HIGS-mediated silencing of a few of them [CL1756Contig1; subtilisin-like protease and CL1817Contig2; 2OG-Fe(II) oxygenase] compromised the pathogenesis of R. solani in tomato.”
t0“Tomato”
Claim · model-written summary
Genes coregulated with RS_CRZ1 during pathogenicity were identified.
- Taxon
- TomatoSolanum lycopersicumt0
- Interactor
- R. solaniRhizoctonia solanie0pathogen
- Gene
- RS_CRZ1e0
- Outcome
- R. solani genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Furthermore, we identified the R. solani genes that were coregulated with RS_CRZ1 during the pathogenicity process.”
t0“Tomato”
Claim · model-written summary
Ectopic RS_CRZ1 expression complemented yeast crz1 and restored metal ion stress tolerance.
- Taxon
- TomatoSolanum lycopersicumt0
- Gene
- RS_CRZ1e0
- Outcome
- restored tolerance against various metal ion stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ectopic expression of RS_CRZ1 complemented the crz1 mutant of yeast and restored tolerance against various metal ion stress.”
t0“Tomato”
The same questions in other papers
loss of function · Rhizoctonia solani · Solanum lycopersicum (tomato)Positive and negative results
2 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
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