Abstract
Abstract Phytophthora capsici is a notorious fungus which infects many crop plants at their early and late growth stages. In the present study, twelve P. capsici isolates were morphologically characterized, and based on pathogenicity assays; two highly virulent isolates causing post-emergence damping-off on locally cultivated chilli pepper were screened. Two P. capsici isolates, HydPak1 (MF322868) and HydPk2 (MF322869) were identified based on internal transcribed spacer (ITS) sequence homology. Plant growth promoting rhizobacteria (PGPR) play a significant role in disease suppression and plant growth promotion in various crops. Out of fifteen bacterial strains recovered from chilli rhizosphere, eight were found potential antagonists to P. capsici in vitro. Bacterial strains with strong antifungal potential were subjected to biochemical and molecular analysis. All tested bacterial strains, were positive for hydrogen cyanide (HCN), catalase production and indole-3-acetic acid (IAA) production (ranging from 6.10 to 56.23 µg ml −1 ), while siderophore production varied between 12.5 and 33.5%. The 16S rRNA sequence analysis of tested bacterial strains showed 98–100% identity with Pseudomonas putida , P. libanensis , P. aeruginosa , Bacillus subtilis , B. megaterium , and B. cereus sequences available in the National Center for Biotechnology Information (NCBI) GenBank nucleotide database. All sequences of identified bacteria were submitted to GenBank for accessions numbers (MH796347-50, MH796355-56, MH801129 and MH801071). Greenhouse studies concluded that all tested bacterial strains significantly suppressed the P. capsici infections (52.3–63%) and enhanced the plant growth characters in chilli pepper. Efficacy of many of these tested rhizobacteria is being first time reported against P. capsici from Pakistan. Plant growth promoting rhizobacteria (PGPR) exhibiting multiple traits may be used in the development of new, eco-friendly, and effective bioformulations as an alternative to synthetic fungicides.
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Claims 10
Claim · model-written summary
Two P. capsici isolates caused post-emergence damping-off in chilli pepper.
Verbatim
e0“two highly virulent isolates causing post-emergence damping-off on locally cultivated chilli pepper”
e1“twelve P. capsici isolates were morphologically characterized”
Claim · model-written summary
HydPak1 and HydPk2 P. capsici isolates were identified by ITS sequence homology.
Verbatim
e0“Two P. capsici isolates, HydPak1 (MF322868) and HydPk2 (MF322869) were identified based on internal transcribed spacer (ITS) sequence homology”
t0“chilli pepper (Capsicum annum L.)”
Claim · model-written summary
Eight of fifteen chilli rhizosphere bacterial strains antagonized P. capsici in vitro.
Verbatim
e0“Out of fifteen bacterial strains recovered from chilli rhizosphere, eight were found potential antagonists to P. capsici in vitro”
Claim · model-written summary
All tested bacterial strains produced HCN, catalase and IAA.
Verbatim
e0“All tested bacterial strains, were positive for hydrogen cyanide (HCN), catalase production and indole-3-acetic acid (IAA) production (ranging from 6.10 to 56.23 µg ml −1 )”
t0“chilli pepper (Capsicum annum L.)”
Claim · model-written summary
Bacterial siderophore production ranged from 12.5 to 33.5%.
Verbatim
e0“siderophore production varied between 12.5 and 33.5%”
e1“Bacterial strains with strong antifungal potential”
t0“chilli pepper (Capsicum annum L.)”
Claim · model-written summary
Tested strains had 98–100% 16S identity with named Pseudomonas and Bacillus species.
Verbatim
e0“98–100% identity with Pseudomonas putida , P. libanensis , P. aeruginosa , Bacillus subtilis , B. megaterium , and B. cereus sequences”
t0“chilli pepper (Capsicum annum L.)”
Claim · model-written summary
Identified bacterial sequences were deposited in GenBank.
Verbatim
e0“All sequences of identified bacteria were submitted to GenBank for accessions numbers (MH796347-50, MH796355-56, MH801129 and MH801071)”
t0“chilli pepper (Capsicum annum L.)”
Claim · model-written summary
Tested strains significantly suppressed P. capsici infections by 52.3–63% in greenhouse chilli.
- Taxon
- chilli peppere0
- Treatment
- all tested bacterial strainse0
- Interactor
- P. capsicie0pathogen
- Second interactor
- bacterial strainse0biocontrol_agent
- Outcome
- P. capsici infectionse0
- Direction
- suppressede0
- Value
- 52.3–63%e0
- Setting
- Greenhousee0
Not reportedComparator
Verbatim
e0“Greenhouse studies concluded that all tested bacterial strains significantly suppressed the P. capsici infections (52.3–63%) and enhanced the plant growth characters in chilli pepper”
Claim · model-written summary
Tested bacterial strains enhanced chilli growth characters in greenhouse studies.
- Taxon
- chilli peppere0
- Treatment
- all tested bacterial strainse0
- Interactor
- bacterial strainse0biocontrol_agent
- Outcome
- plant growth characterse0
- Direction
- enhancede0
- Setting
- Greenhousee0
Not reportedComparator
Verbatim
e0“Greenhouse studies concluded that all tested bacterial strains significantly suppressed the P. capsici infections (52.3–63%) and enhanced the plant growth characters in chilli pepper”
Claim · model-written summary
The rhizobacteria may be used in eco-friendly bioformulations as alternatives to synthetic fungicides.
Verbatim
e0“Plant growth promoting rhizobacteria (PGPR) exhibiting multiple traits may be used in the development of new, eco-friendly, and effective bioformulations as an alternative to synthetic fungicides”
t0“chilli pepper (Capsicum annum L.)”
The same questions in other papers
inoculation · growth · Capsicum (peppers)Positive and negative results
47 papers in the same direction as this paper · 8 in the opposite direction
- positive
- no effect
- no effect
52 papers more in this group
See all 56 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.