Abstract
Abstract Selenium (Se) causes oxidative damage to plants. Proline is accumulated as a compatible solute in plants under stress conditions and mitigates stresses. Selenate at 250 µM increased cell death and inhibited the growth of tobacco BY-2 cells while exogenous proline at 10 mM did not mitigate the inhibition by selenate. Selenate increased accumulation of Se and ROS and activities of antioxidant enzymes but not lipid peroxidation in the BY-2 cells. Proline increased Se accumulation and antioxidant enzyme activities but not either ROS accumulation or lipid peroxidation in the selenate-stressed cells. Glutathione (GSH) rather than ascorbic acid (AsA) mitigated the growth inhibition although both reduced the accumulation of ROS induced by selenate. These results indicate that proline increases both antioxidant enzyme activities and Se accumulation, which overall fails to ameliorate the growth inhibition by selenate and that the growth inhibition is not accounted for only by ROS accumulation. Abbreviations: APX: ascorbate peroxidase; AsA: ascorbic acid; BY-2: Bright Yellow-2; CAT: catalase; DAI: days after inoculation; DW: dry weight; FW: fresh weight; GSH: glutathione; ROS: reactive oxygen species
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Claims 15
Claim · model-written summary
Selenate increased tobacco BY-2 cell death.
- Taxon
- tobacco
Nicotiana tabacume0 - Material
- BY-2e0
- Treatment
- Selenate at 250 µMe0
- Outcome
- cell deathe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Selenate at 250 µM increased cell death and inhibited the growth of tobacco BY-2 cells”
Claim · model-written summary
Selenate inhibited tobacco BY-2 growth.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- BY-2e0
- Treatment
- Selenate at 250 µMe0
- Outcome
- growthe0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Selenate at 250 µM increased cell death and inhibited the growth of tobacco BY-2 cells”
Claim · model-written summary
Proline at 10 mM did not mitigate selenate growth inhibition.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- exogenous proline at 10 mMe0
- Outcome
- inhibition by selenatee0
- Direction
- did not mitigatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous proline at 10 mM did not mitigate the inhibition by selenate.”
e1“tobacco BY-2 cells”
Claim · model-written summary
Selenate increased Se accumulation in tobacco BY-2 cells.
- Taxon
- tobaccoNicotiana tabacume1
- Material
- BY-2e0
- Treatment
- Selenatee0
- Outcome
- accumulation of See0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Selenate increased accumulation of Se and ROS and activities of antioxidant enzymes but not lipid peroxidation in the BY-2 cells.”
e1“tobacco BY-2 cells”
Claim · model-written summary
Selenate increased ROS accumulation in tobacco BY-2 cells.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Selenate increased accumulation of Se and ROS and activities of antioxidant enzymes but not lipid peroxidation in the BY-2 cells.”
e1“tobacco BY-2 cells”
Claim · model-written summary
Selenate increased antioxidant enzyme activities in tobacco BY-2 cells.
- Taxon
- tobaccoNicotiana tabacume1
- Material
- BY-2e0
- Treatment
- Selenatee0
- Outcome
- activities of antioxidant enzymese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Selenate increased accumulation of Se and ROS and activities of antioxidant enzymes but not lipid peroxidation in the BY-2 cells.”
e1“tobacco BY-2 cells”
Claim · model-written summary
Selenate did not increase lipid peroxidation.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- Selenatee0
- Outcome
- lipid peroxidatione0
- Direction
- note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Selenate increased accumulation of Se and ROS and activities of antioxidant enzymes but not lipid peroxidation in the BY-2 cells.”
e1“tobacco BY-2 cells”
Claim · model-written summary
Proline increased Se accumulation in selenate-stressed tobacco cells.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- Prolinee0
- Outcome
- Se accumulatione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Proline increased Se accumulation and antioxidant enzyme activities but not either ROS accumulation or lipid peroxidation in the selenate-stressed cells.”
e1“tobacco BY-2 cells”
Claim · model-written summary
Proline increased antioxidant enzyme activities in selenate-stressed tobacco cells.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- Prolinee0
- Outcome
- antioxidant enzyme activitiese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Proline increased Se accumulation and antioxidant enzyme activities but not either ROS accumulation or lipid peroxidation in the selenate-stressed cells.”
e1“tobacco BY-2 cells”
Claim · model-written summary
Proline did not increase ROS accumulation in selenate-stressed cells.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- Prolinee0
- Outcome
- ROS accumulatione0
- Direction
- note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Proline increased Se accumulation and antioxidant enzyme activities but not either ROS accumulation or lipid peroxidation in the selenate-stressed cells.”
e1“tobacco BY-2 cells”
Claim · model-written summary
Proline did not increase lipid peroxidation in selenate-stressed cells.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- Prolinee0
- Outcome
- lipid peroxidatione0
- Direction
- note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Proline increased Se accumulation and antioxidant enzyme activities but not either ROS accumulation or lipid peroxidation in the selenate-stressed cells.”
e1“tobacco BY-2 cells”
Claim · model-written summary
Glutathione mitigated selenate growth inhibition.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- Glutathione (GSH)e0
- Outcome
- growth inhibitione0
- Direction
- mitigatede0
- Comparator
- ascorbic acid (AsA)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Glutathione (GSH) rather than ascorbic acid (AsA) mitigated the growth inhibition”
e1“tobacco BY-2 cells”
Claim · model-written summary
Both glutathione and ascorbic acid reduced selenate-induced ROS accumulation.
- Taxon
- tobaccoNicotiana tabacume2
- Treatment
- Glutathione (GSH)e1
- Outcome
- accumulation of ROSe0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both reduced the accumulation of ROS induced by selenate”
e1“Glutathione (GSH) rather than ascorbic acid (AsA)”
e2“tobacco BY-2 cells”
Claim · model-written summary
Ascorbic acid reduced selenate-induced ROS accumulation.
- Taxon
- tobaccoNicotiana tabacume2
- Treatment
- ascorbic acid (AsA)e1
- Outcome
- accumulation of ROSe0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both reduced the accumulation of ROS induced by selenate”
e1“ascorbic acid (AsA)”
e2“tobacco BY-2 cells”
Claim · model-written summary
The authors concluded ROS accumulation alone does not explain selenate growth inhibition.
- Taxon
- tobaccoNicotiana tabacume1
- Outcome
- growth inhibitione0
- Qualifier
- ROS accumulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the growth inhibition is not accounted for only by ROS accumulation”
e1“tobacco BY-2 cells”
The same questions in other papers
L-ascorbic acid · physiology: oxidative · Nicotiana tabacum (tobacco)Positive and negative results
1 in the opposite direction
- negative
proline · disease · Nicotiana tabacum (tobacco)Positive and negative results
2 in the opposite direction
- positive
- positive
glutathione · disease · Nicotiana tabacum (tobacco)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
proline · physiology: oxidative · Nicotiana tabacum (tobacco)Positive and negative results
3 in the opposite direction
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.