Abstract
The effect of exogenous 5-aminolevulinic acid (ALA) on the activity of dihydroflavonol-4-reductase (DFR), the expression of the dfr gene and the hy5 gene of the transcription factor Hy5 and the light effect of different intensities in combination with the ALA action on the accumulation of anthocyanins in cotyledonous leaves of winter rape (Brassica napus L.) were studied. It was shown that the stimulation of the accumulation of anthocyanins under the exogenous ALA action at the molecular level was provided by increasing the expression level of the dfr and hy5 genes and the activity of the DFR enzyme. Increasing the light intensity from 40.5 to 66.2 μmol photons/m 2 ·s enhanced the ability of plants to accumulate anthocyanins on average by 35 %. The ALA action at concentrations of 50, 100, 150 and 200 mg/L led to an additional increase in the accumulation of anthocyanins at the two used levels of illumination, and in a dose-dependent manner. The stimulating effect of ALA under high light intensity was much higher than in the case of lower illumination. Thus, the stimulation of the anthocyanin accumulation under illumination of 40.5 μmol photons/m 2 ·s was 106 % when using 50 mg/L ALA, 165 % – when using 100 mg/L ALA, 222 % – in the case of 150 mg/L ALA and 350 % – under the action of 200 mg/L ALA compared with light control without of ALA treatment. At an illumination of 66.2 μmol photons/m 2 ·s, these indicators were 164, 262, 359 and 583 % respectively. Thus, it was demonstrated that the stimulation of the accumulation of anthocyanins under the action of ALA in winter rape plants was due to its positive effect on the transcription of the dfr and hy5 genes at the molecular level.
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Claims 14
Claim · model-written summary
Exogenous ALA increased the expression level of the dfr and hy5 genes in winter rape.
- Taxon
- winter rape plants
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napust0 - Treatment
- exogenous ALA actione1
- Gene
- dfre1
- Outcome
- expression level of the dfr and hy5 genese1
- Direction
- increasinge1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
t0“winter rape plants”
e1“It was shown that the stimulation of the accumulation of anthocyanins under the exogenous ALA action at the molecular level was provided by increasing the expression level of the dfr and hy5 genes and the activity of the DFR enzyme”
Claim · model-written summary
Exogenous ALA increased the activity of the DFR enzyme in winter rape.
- Taxon
- winter rape plantsBrassica napust0
- Treatment
- exogenous ALA actione1
- Gene
- DFR enzymee1
- Outcome
- activity of the DFR enzymee1
- Direction
- increasinge1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“winter rape plants”
e1“It was shown that the stimulation of the accumulation of anthocyanins under the exogenous ALA action at the molecular level was provided by increasing the expression level of the dfr and hy5 genes and the activity of the DFR enzyme”
Claim · model-written summary
The stimulation of anthocyanin accumulation by ALA was attributed to its positive effect on transcription of the dfr and hy5 genes.
- Taxon
- winter rape plantsBrassica napuse3
- Treatment
- ALAe3
- Gene
- dfre3
- Outcome
- stimulation of the accumulation of anthocyaninse3
- Qualifier
- positive effect on the transcriptione3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“it was demonstrated that the stimulation of the accumulation of anthocyanins under the action of ALA in winter rape plants was due to its positive effect on the transcription of the dfr and hy5 genes at the molecular level”
Claim · model-written summary
Increasing light intensity from 40.5 to 66.2 μmol photons/m2·s enhanced anthocyanin accumulation by 35 % on average.
- Taxon
- winter rape plantsBrassica napust0
- Treatment
- Increasing the light intensity from 40.5 to 66.2 μmol photons/m 2 ·se4
- Outcome
- ability of plants to accumulate anthocyaninse4
- Direction
- enhancede4
- Value
- 35 %e4
- Comparator
- 40.5e4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“winter rape plants”
e4“Increasing the light intensity from 40.5 to 66.2 μmol photons/m 2 ·s enhanced the ability of plants to accumulate anthocyanins on average by 35 %”
Claim · model-written summary
ALA at 50, 100, 150 and 200 mg/L increased anthocyanin accumulation in a dose-dependent manner.
- Taxon
- winter rape plantsBrassica napust0
- Treatment
- The ALA action at concentrations of 50, 100, 150 and 200 mg/Le5
- Outcome
- accumulation of anthocyaninse5
- Direction
- increasee5
- Qualifier
- in a dose-dependent mannere5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“winter rape plants”
e5“The ALA action at concentrations of 50, 100, 150 and 200 mg/L led to an additional increase in the accumulation of anthocyanins at the two used levels of illumination, and in a dose-dependent manner”
Claim · model-written summary
The stimulating effect of ALA on anthocyanin accumulation was higher under high light than under lower illumination.
- Taxon
- winter rape plantsBrassica napust0
- Treatment
- ALAe6
- Outcome
- stimulating effecte6
- Direction
- highere6
- Qualifier
- under high light intensitye6
- Comparator
- lower illuminatione6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“winter rape plants”
e6“The stimulating effect of ALA under high light intensity was much higher than in the case of lower illumination”
Claim · model-written summary
At 40.5 μmol photons/m2·s, ALA at 50 mg/L stimulated anthocyanin accumulation by 106 % relative to light control without ALA.
- Taxon
- winter rape plantsBrassica napust0
- Treatment
- 50 mg/L ALAe7
- Outcome
- stimulation of the anthocyanin accumulatione7
- Direction
- increaseinferrede7
- Value
- 106 %e7
- Comparator
- light control without of ALA treatmente0
- Setting
- illumination of 40.5 μmol photons/m 2 ·se7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“winter rape plants”
e7“Thus, the stimulation of the anthocyanin accumulation under illumination of 40.5 μmol photons/m 2 ·s was 106 % when using 50 mg/L ALA”
e0“compared with light control without of ALA treatment”
Claim · model-written summary
At 40.5 μmol photons/m2·s, ALA at 100 mg/L stimulated anthocyanin accumulation by 165 % relative to light control without ALA.
- Taxon
- winter rape plantsBrassica napust0
- Treatment
- 100 mg/L ALAe1
- Outcome
- stimulation of the anthocyanin accumulatione7
- Direction
- increaseinferrede7
- Value
- 165 %e1
- Comparator
- light control without of ALA treatmente0
- Setting
- illumination of 40.5 μmol photons/m 2 ·se7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“winter rape plants”
e7“Thus, the stimulation of the anthocyanin accumulation under illumination of 40.5 μmol photons/m 2 ·s was 106 % when using 50 mg/L ALA”
e0“compared with light control without of ALA treatment”
e1“165 % – when using 100 mg/L ALA”
Claim · model-written summary
At 40.5 μmol photons/m2·s, ALA at 150 mg/L stimulated anthocyanin accumulation by 222 % relative to light control without ALA.
- Taxon
- winter rape plantsBrassica napust0
- Treatment
- 150 mg/L ALAe2
- Outcome
- stimulation of the anthocyanin accumulatione7
- Direction
- increaseinferrede7
- Value
- 222 %e2
- Comparator
- light control without of ALA treatmente0
- Setting
- illumination of 40.5 μmol photons/m 2 ·se7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“winter rape plants”
e7“Thus, the stimulation of the anthocyanin accumulation under illumination of 40.5 μmol photons/m 2 ·s was 106 % when using 50 mg/L ALA”
e0“compared with light control without of ALA treatment”
e2“222 % – in the case of 150 mg/L ALA”
Claim · model-written summary
At 40.5 μmol photons/m2·s, ALA at 200 mg/L stimulated anthocyanin accumulation by 350 % relative to light control without ALA.
- Taxon
- winter rape plantsBrassica napust0
- Treatment
- 200 mg/L ALAe3
- Outcome
- stimulation of the anthocyanin accumulatione7
- Direction
- increaseinferrede7
- Value
- 350 %e3
- Comparator
- light control without of ALA treatmente0
- Setting
- illumination of 40.5 μmol photons/m 2 ·se7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“winter rape plants”
e7“Thus, the stimulation of the anthocyanin accumulation under illumination of 40.5 μmol photons/m 2 ·s was 106 % when using 50 mg/L ALA”
e0“compared with light control without of ALA treatment”
e3“350 % – under the action of 200 mg/L ALA”
Claim · model-written summary
At 66.2 μmol photons/m2·s, ALA (the first of four concentrations, 50 mg/L) increased anthocyanin accumulation by 164 %.
- Taxon
- winter rape plantsBrassica napust0
- Treatment
- ALA actione5
- Outcome
- these indicatorse4
- Direction
- increaseinferrede4
- Value
- 164e4
- Setting
- illumination of 66.2 μmol photons/m 2 ·se4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“winter rape plants”
e4“At an illumination of 66.2 μmol photons/m 2 ·s, these indicators were 164, 262, 359 and 583 % respectively”
e5“The ALA action at concentrations of 50, 100, 150 and 200 mg/L”
Claim · model-written summary
At 66.2 μmol photons/m2·s, ALA (second concentration, 100 mg/L) increased anthocyanin accumulation by 262 %.
- Taxon
- winter rape plantsBrassica napust0
- Treatment
- ALA actione5
- Outcome
- these indicatorse4
- Direction
- increaseinferrede4
- Value
- 262e4
- Setting
- illumination of 66.2 μmol photons/m 2 ·se4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“winter rape plants”
e4“At an illumination of 66.2 μmol photons/m 2 ·s, these indicators were 164, 262, 359 and 583 % respectively”
e5“The ALA action at concentrations of 50, 100, 150 and 200 mg/L”
Claim · model-written summary
At 66.2 μmol photons/m2·s, ALA (third concentration, 150 mg/L) increased anthocyanin accumulation by 359 %.
- Taxon
- winter rape plantsBrassica napust0
- Treatment
- ALA actione5
- Outcome
- these indicatorse4
- Direction
- increaseinferrede4
- Value
- 359e4
- Setting
- illumination of 66.2 μmol photons/m 2 ·se4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“winter rape plants”
e4“At an illumination of 66.2 μmol photons/m 2 ·s, these indicators were 164, 262, 359 and 583 % respectively”
e5“The ALA action at concentrations of 50, 100, 150 and 200 mg/L”
Claim · model-written summary
At 66.2 μmol photons/m2·s, ALA (fourth concentration, 200 mg/L) increased anthocyanin accumulation by 583 %.
- Taxon
- winter rape plantsBrassica napust0
- Treatment
- ALA actione5
- Outcome
- these indicatorse4
- Direction
- increaseinferrede4
- Value
- 583 %e4
- Setting
- illumination of 66.2 μmol photons/m 2 ·se4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“winter rape plants”
e4“At an illumination of 66.2 μmol photons/m 2 ·s, these indicators were 164, 262, 359 and 583 % respectively”
e5“The ALA action at concentrations of 50, 100, 150 and 200 mg/L”
The same questions in other papers
5-aminolevulinic acid · molecular: expression · Brassica napus (oilseed rape)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
photon · quality: phytochemicals · Brassica napus (oilseed rape)Results agree
1 paper in the same direction as this paper
- positive
5-aminolevulinic acid · quality: phytochemicals · Brassica napus (oilseed rape)Results agree
1 paper in the same direction as this paper
- positive
light · quality: phytochemicals · Brassica napus (oilseed rape)Positive and negative results
5 papers in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.