Abstract
Two strains of 64 endophytic bacteria, Bacillus cereus Si-Ps1 and Pseudomonas azotoformans La-Pot3-3, isolated from Citrus sinensis and C. sinensis var. Thomson's leaves, respectively, reduced N-acyl homoserine-based quorum sensing in bioindicator strain Pseudomonas syringae pv. syringae (Pss) B728a and the biofilm production and swarming motility of field isolate Pss 3289. A homolog of aiiA gene encoding an AHL-lactonase was found in B. cereus (Si-Ps1), suggesting that this isolate can degrade the quorum-sensing signal molecules of Pss 3289. The crude extract of endophytic bacterium, B. cereus (Si-Ps1), inhibited Pss 3289 biofilm formation after 48 and 96 h by 55% and 58%, respectively. Similar reductions in biofilm formation were conferred by crude extracts of P. azotoformans (La-Pot3-3). Correspondingly, the number of planktonic cells in cultures treated with these extracts was higher than in control cultures, indicating a direct effect on biofilm formation and not on cell growth. In greenhouse assays, the virulence of Pss 3289 to different citrus cultivars was decreased when coinoculated with these endophytic bacteria.
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Claims 8
Claim · model-written summary
Bacillus cereus Si-Ps1 reduced quorum sensing in the Pss bioindicator.
- Taxon
- Citrus sinensis
Citrus sinensise0 - Treatment
- Bacillus cereus Si-Ps1e0
- Interactor
- Pseudomonas syringae pv. syringae (Pss) B728a
Pseudomonas syringae pv. syringaee0pathogen - Second interactor
- Bacillus cereus Si-Ps1
Bacillus cereuse0biocontrol_agent - Outcome
- N-acyl homoserine-based quorum sensinge0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Bacillus cereus Si-Ps1 and Pseudomonas azotoformans La-Pot3-3, isolated from Citrus sinensis and C. sinensis var. Thomson's leaves, respectively, reduced N-acyl homoserine-based quorum sensing in bioindicator strain Pseudomonas syringae pv. syringae (Pss) B728a”
Claim · model-written summary
Pseudomonas azotoformans La-Pot3-3 reduced quorum sensing in the Pss bioindicator.
- Taxon
- C. sinensis var. Thomson'sCitrus sinensise0
- Treatment
- Pseudomonas azotoformans La-Pot3-3e0
- Interactor
- Pseudomonas syringae pv. syringae (Pss) B728aPseudomonas syringae pv. syringaee0pathogen
- Second interactor
- Pseudomonas azotoformans La-Pot3-3Pseudomonas azotoformanse0biocontrol_agent
- Outcome
- N-acyl homoserine-based quorum sensinge0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Bacillus cereus Si-Ps1 and Pseudomonas azotoformans La-Pot3-3, isolated from Citrus sinensis and C. sinensis var. Thomson's leaves, respectively, reduced N-acyl homoserine-based quorum sensing in bioindicator strain Pseudomonas syringae pv. syringae (Pss) B728a”
Claim · model-written summary
B. cereus Si-Ps1 contains an aiiA homolog, suggesting AHL signal degradation.
- Taxon
- Citrus sinensisCitrus sinensise1
- Interactor
- Pss 3289e0pathogen
- Second interactor
- B. cereus (Si-Ps1)Bacillus cereuse0biocontrol_agent
- Gene
- aiiAe0
- Outcome
- A homolog of aiiA gene encoding an AHL-lactonasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A homolog of aiiA gene encoding an AHL-lactonase was found in B. cereus (Si-Ps1), suggesting that this isolate can degrade the quorum-sensing signal molecules of Pss 3289”
e1“isolated from Citrus sinensis and C. sinensis var. Thomson's leaves”
Claim · model-written summary
B. cereus extract inhibited Pss biofilm formation by 55% after 48 hours.
- Taxon
- Citrus sinensisCitrus sinensise1
- Treatment
- crude extract of endophytic bacterium, B. cereus (Si-Ps1)e0
- Interactor
- Pss 3289e0pathogen
- Second interactor
- B. cereus (Si-Ps1)Bacillus cereuse0biocontrol_agent
- Outcome
- Pss 3289 biofilm formatione0
- Direction
- inhibitede0
- Value
- 55%e0
- Qualifier
- 48e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The crude extract of endophytic bacterium, B. cereus (Si-Ps1), inhibited Pss 3289 biofilm formation after 48 and 96 h by 55% and 58%, respectively”
e1“isolated from Citrus sinensis and C. sinensis var. Thomson's leaves”
Claim · model-written summary
B. cereus extract inhibited Pss biofilm formation by 58% after 96 hours.
- Taxon
- Citrus sinensisCitrus sinensise1
- Treatment
- crude extract of endophytic bacterium, B. cereus (Si-Ps1)e0
- Interactor
- Pss 3289e0pathogen
- Second interactor
- B. cereus (Si-Ps1)Bacillus cereuse0biocontrol_agent
- Outcome
- Pss 3289 biofilm formatione0
- Direction
- inhibitede0
- Value
- 58%e0
- Qualifier
- 96 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The crude extract of endophytic bacterium, B. cereus (Si-Ps1), inhibited Pss 3289 biofilm formation after 48 and 96 h by 55% and 58%, respectively”
e1“isolated from Citrus sinensis and C. sinensis var. Thomson's leaves”
Claim · model-written summary
P. azotoformans extract similarly reduced Pss biofilm formation.
- Taxon
- C. sinensis var. Thomson'sCitrus sinensise2
- Treatment
- crude extracts of P. azotoformans (La-Pot3-3)e0
- Interactor
- Pss 3289e1pathogen
- Second interactor
- P. azotoformans (La-Pot3-3)Pseudomonas azotoformanse0biocontrol_agent
- Outcome
- Pss 3289 biofilm formatione1
- Direction
- reductionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Similar reductions in biofilm formation were conferred by crude extracts of P. azotoformans (La-Pot3-3)”
e1“The crude extract of endophytic bacterium, B. cereus (Si-Ps1), inhibited Pss 3289 biofilm formation”
e2“C. sinensis var. Thomson's leaves”
Claim · model-written summary
Extract-treated cultures had more planktonic cells than controls.
- Taxon
- Citrus sinensisCitrus sinensise2
- Treatment
- these extractse0
- Interactor
- Pss 3289e1pathogen
- Outcome
- number of planktonic cellse0
- Direction
- highere0
- Comparator
- control culturese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the number of planktonic cells in cultures treated with these extracts was higher than in control cultures”
e1“the biofilm production and swarming motility of field isolate Pss 3289”
e2“isolated from Citrus sinensis and C. sinensis var. Thomson's leaves”
Claim · model-written summary
Coinoculation with the endophytes decreased Pss virulence on citrus cultivars in greenhouse assays.
- Treatment
- coinoculated with these endophytic bacteriae0
- Interactor
- Pss 3289e0pathogen
- Outcome
- virulence of Pss 3289e0
- Direction
- decreasede0
- Setting
- greenhouse assayse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In greenhouse assays, the virulence of Pss 3289 to different citrus cultivars was decreased when coinoculated with these endophytic bacteria”
The same questions in other papers
inoculation · disease · CitrusPositive and negative results
10 papers in the same direction as this paper · 12 in the opposite direction
- no effect
- negative
- negative
19 papers more in this group
See all 23 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.