Abstract
Abstract Epiphytic yeasts were isolated from different cultivars of apples and lemons and identified by a combination of PCR‐RFLP of 5.8S rRNA region and sequencing of D1/D2 domain of the 26S rRNA gene. Among 69 isolates, Aureobasidium pullulans GE17 and Meyerozyma guilliermondii KL3 strains showed the greatest antagonistic activity against two significant apple and lemon postharvest pathogens, Penicillium expansum DSM62841 (blue mold) and Penicillium digitatum DSM2750 (green mold), after preliminary screening. Yeasts were applied as single and mixed cultures with two different cell concentrations of 10 6 and 10 8 cells/ml in the present study. It was determined that antagonistic activity of two yeast strains studied emerged with a combination of several mechanisms of action including competition for space and nutrients, production of volatile organic compounds (VOCs), secretion of extracellular lytic enzymes and inhibition of fungal spore germination. The highest inhibition of mycelial growth on P. expansum DSM62841 and P. digitatum DSM2750 (83.4% and 74.7%, respectively) was achieved by utilization of single culture of A. pullulans GE17. Otherwise, the application of mixed culture at the ratio of 10 8 cells/ml inhibited spore germination of both pathogens from 86% to 95%. Results of this study suggest that an increase in yeast cell concentrations positively affected their biocontrol activity against blue and green molds. According to the results, employing single culture of M. guilliermondii KL3 did not exhibit effective antagonistic activity against blue and green molds. However, utilization of A. pullulans GE17 alone and mixed culture showed succesfull controlling against both P. expansum DSM62841 and P. digitatum DSM2750.
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Claims 8
Claim · model-written summary
Single culture of A. pullulans GE17 gave the highest inhibition of mycelial growth of P. expansum DSM62841 (83.4%).
- Taxon
- apples and lemons
Citrus ×limon
Malus domesticae0 - Treatment
- single culture of A. pullulans GE17e1
- Interactor
- P. expansum DSM62841
Penicillium expansume1pathogen - Second interactor
- A. pullulans GE17
Aureobasidium pullulanse1biocontrol_agent - Outcome
- inhibition of mycelial growthe1
- Direction
- higheste1
- Value
- 83.4%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“isolated from different cultivars of apples and lemons”
e1“The highest inhibition of mycelial growth on P. expansum DSM62841 and P. digitatum DSM2750 (83.4% and 74.7%, respectively) was achieved by utilization of single culture of A. pullulans GE17”
Claim · model-written summary
Single culture of A. pullulans GE17 gave the highest inhibition of mycelial growth of P. digitatum DSM2750 (74.7%).
- Taxon
- apples and lemonsCitrus ×limonMalus domesticae0
- Treatment
- single culture of A. pullulans GE17e1
- Interactor
- P. digitatum DSM2750
Penicillium digitatume1pathogen - Second interactor
- A. pullulans GE17Aureobasidium pullulanse1biocontrol_agent
- Outcome
- inhibition of mycelial growthe1
- Direction
- higheste1
- Value
- 74.7%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“isolated from different cultivars of apples and lemons”
e1“The highest inhibition of mycelial growth on P. expansum DSM62841 and P. digitatum DSM2750 (83.4% and 74.7%, respectively) was achieved by utilization of single culture of A. pullulans GE17”
Claim · model-written summary
Mixed culture of the two yeasts at 10^8 cells/ml inhibited spore germination of P. expansum and P. digitatum from 86% to 95%.
- Taxon
- apples and lemonsCitrus ×limonMalus domesticae0
- Treatment
- mixed culture at the ratio of 10 8 cells/mle2
- Interactor
- Penicillium expansumPenicillium expansume7pathogen
- Second interactor
- Penicillium digitatumPenicillium digitatume7pathogen
- Outcome
- spore germinatione2
- Direction
- inhibitede2
- Value
- 86% to 95%e2
- Qualifier
- both pathogense2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“isolated from different cultivars of apples and lemons”
e2“the application of mixed culture at the ratio of 10 8 cells/ml inhibited spore germination of both pathogens from 86% to 95%”
e7“Penicillium expansum DSM62841 (blue mold) and Penicillium digitatum DSM2750 (green mold)”
Claim · model-written summary
Increasing yeast cell concentration positively affected biocontrol activity against blue and green molds.
- Taxon
- apples and lemonsCitrus ×limonMalus domesticae0
- Treatment
- increase in yeast cell concentrationse3
- Interactor
- blue and green moldse3pathogen
- Outcome
- biocontrol activitye3
- Direction
- positively affectede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“isolated from different cultivars of apples and lemons”
e3“an increase in yeast cell concentrations positively affected their biocontrol activity against blue and green molds”
Claim · model-written summary
Single culture of M. guilliermondii KL3 did not show effective antagonistic activity against blue and green molds.
- Taxon
- apples and lemonsCitrus ×limonMalus domesticae0
- Treatment
- single culture of M. guilliermondii KL3e4
- Interactor
- blue and green moldse4pathogen
- Second interactor
- M. guilliermondii KL3Meyerozyma guilliermondiie4biocontrol_agent
- Outcome
- effective antagonistic activitye4
- Direction
- did not exhibite4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“isolated from different cultivars of apples and lemons”
e4“employing single culture of M. guilliermondii KL3 did not exhibit effective antagonistic activity against blue and green molds”
Claim · model-written summary
A. pullulans GE17 alone and in mixed culture gave successful control of P. expansum DSM62841.
- Taxon
- apples and lemonsCitrus ×limonMalus domesticae0
- Treatment
- A. pullulans GE17 alone and mixed culturee5
- Interactor
- P. expansum DSM62841Penicillium expansume5pathogen
- Second interactor
- A. pullulans GE17Aureobasidium pullulanse5biocontrol_agent
- Outcome
- succesfull controllinge5
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“isolated from different cultivars of apples and lemons”
e5“utilization of A. pullulans GE17 alone and mixed culture showed succesfull controlling against both P. expansum DSM62841 and P. digitatum DSM2750”
Claim · model-written summary
A. pullulans GE17 alone and in mixed culture gave successful control of P. digitatum DSM2750.
- Taxon
- apples and lemonsCitrus ×limonMalus domesticae0
- Treatment
- A. pullulans GE17 alone and mixed culturee5
- Interactor
- P. digitatum DSM2750Penicillium digitatume5pathogen
- Second interactor
- A. pullulans GE17Aureobasidium pullulanse5biocontrol_agent
- Outcome
- succesfull controllinge5
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“isolated from different cultivars of apples and lemons”
e5“utilization of A. pullulans GE17 alone and mixed culture showed succesfull controlling against both P. expansum DSM62841 and P. digitatum DSM2750”
Claim · model-written summary
Among 69 yeast isolates, A. pullulans GE17 and M. guilliermondii KL3 showed the greatest antagonistic activity.
- Taxon
- apples and lemonsCitrus ×limonMalus domesticae0
- Material
- Aureobasidium pullulans GE17 and Meyerozyma guilliermondii KL3e6
- Outcome
- greatest antagonistic activitye6
- Qualifier
- Among 69 isolatese6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“isolated from different cultivars of apples and lemons”
e6“Among 69 isolates, Aureobasidium pullulans GE17 and Meyerozyma guilliermondii KL3 strains showed the greatest antagonistic activity”
The same questions in other papers
Aureobasidium pullulans · Penicillium expansum · Malus domestica (apple)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.