Abstract
Circular dichroism (CD), defined as the differential absorption of left- and right-handed circularly polarized light (CPL), is a useful spectroscopic technique for structural studies of biological systems composed of chiral molecules. The present study evaluated the effects of CPL on germination, hypocotyl elongation and biomass production of Arabidopsis and lettuce. Higher germination rates were observed when Arabidopsis and lettuce seedlings were irradiated with red right-handed CPL (R-CPL) than with red left-handed CPL (L-CPL). Hypocotyl elongation was effectively inhibited when Arabidopsis and lettuce seedlings were irradiated with red R-CPL than with red L-CPL. This difference was not observed when a phytochrome B (phyB) deficient mutant of Arabidopsis was irradiated, suggesting that inhibition of elongation by red R-CPL was mediated by phyB. White R-CPL induced greater biomass production by adult Arabidopsis plants, as determined by their fresh shoot weight, than white L-CPL. To determine the molecular basis of these CPL effects, CD spectra and the effect of CPL on the photoreaction of a sensory module of Arabidopsis phyB were measured. The red light-absorbing form of phyB showed a negative CD in the red light-absorbing region, consistent with the results of germination, inhibition of hypocotyl elongation and biomass production. L-CPL and R-CPL, however, did not differ in their ability to induce the interconversion of the red light-absorbing and far-red light-absorbing forms of phyB. These findings suggest that these CPL effects involve phyB, along with other photoreceptors and the photosynthetic process.
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Claims 10
Claim · model-written summary
Red right handed CPL produced higher Arabidopsis germination rates than red left handed CPL.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- red right-handed CPL (R-CPL)e0
- Outcome
- germination ratese0
- Direction
- Highere0
- Comparator
- red left-handed CPL (L-CPL)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Higher germination rates were observed when Arabidopsis and lettuce seedlings were irradiated with red right-handed CPL (R-CPL) than with red left-handed CPL (L-CPL).”
Claim · model-written summary
Red right handed CPL produced higher lettuce germination rates than red left handed CPL.
- Taxon
- lettuce
Lactuca sativae0 - Treatment
- red right-handed CPL (R-CPL)e0
- Outcome
- germination ratese0
- Direction
- Highere0
- Comparator
- red left-handed CPL (L-CPL)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Higher germination rates were observed when Arabidopsis and lettuce seedlings were irradiated with red right-handed CPL (R-CPL) than with red left-handed CPL (L-CPL).”
Claim · model-written summary
Red right handed CPL inhibited Arabidopsis hypocotyl elongation relative to red left handed CPL.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- red R-CPLe0
- Outcome
- Hypocotyl elongatione0
- Direction
- inhibitede0
- Comparator
- red L-CPLe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hypocotyl elongation was effectively inhibited when Arabidopsis and lettuce seedlings were irradiated with red R-CPL than with red L-CPL.”
Claim · model-written summary
Red right handed CPL inhibited lettuce hypocotyl elongation relative to red left handed CPL.
- Taxon
- lettuceLactuca sativae0
- Treatment
- red R-CPLe0
- Outcome
- Hypocotyl elongatione0
- Direction
- inhibitede0
- Comparator
- red L-CPLe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hypocotyl elongation was effectively inhibited when Arabidopsis and lettuce seedlings were irradiated with red R-CPL than with red L-CPL.”
Claim · model-written summary
The elongation difference was absent in phyB deficient Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Material
- phytochrome B (phyB) deficient mutante0
- Gene
- phytochrome B (phyB)e0
- Outcome
- difference was not observede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This difference was not observed when a phytochrome B (phyB) deficient mutant of Arabidopsis was irradiated”
Claim · model-written summary
The authors interpreted the mutant result as suggesting phyB mediates inhibition by red R-CPL.
- Taxon
- arabidopsisArabidopsist0
- Treatment
- red R-CPLe0
- Gene
- phyBe0
- Outcome
- inhibition of elongatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggesting that inhibition of elongation by red R-CPL was mediated by phyB.”
t0“arabidopsis”
Claim · model-written summary
White right handed CPL increased adult Arabidopsis fresh shoot weight over white left handed CPL.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- adulte0
- Treatment
- White R-CPLe0
- Outcome
- fresh shoot weighte0
- Direction
- greatere0
- Comparator
- white L-CPLe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“White R-CPL induced greater biomass production by adult Arabidopsis plants, as determined by their fresh shoot weight, than white L-CPL.”
Claim · model-written summary
The red absorbing form of Arabidopsis phyB showed negative circular dichroism in its red absorbing region.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- phyBe0
- Outcome
- negative CDe0
- Qualifier
- red light-absorbing regione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The red light-absorbing form of phyB showed a negative CD in the red light-absorbing region”
e1“Arabidopsis phyB”
Claim · model-written summary
Left and right CPL did not differ in inducing phyB form interconversion.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- L-CPLe0
- Gene
- phyBe0
- Outcome
- interconversion of the red light-absorbing and far-red light-absorbing forms of phyBe0
- Direction
- did not differe0
- Comparator
- R-CPLe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“L-CPL and R-CPL, however, did not differ in their ability to induce the interconversion of the red light-absorbing and far-red light-absorbing forms of phyB.”
e1“Arabidopsis phyB”
Claim · model-written summary
The authors suggested CPL effects involve phyB, other photoreceptors and photosynthesis.
- Taxon
- arabidopsis and lettuceArabidopsisLactuca sativat0
- Gene
- phyBe0
- Outcome
- CPL effectse0
- Qualifier
- other photoreceptors and the photosynthetic processe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these CPL effects involve phyB, along with other photoreceptors and the photosynthetic process.”
t0“arabidopsis and lettuce”
The same questions in other papers
light · growth · ArabidopsisResults differ
26 papers in the same direction as this paper · 16 in the opposite direction · 1 reporting no difference from an alternative
- negative
- negative
- negative
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See all 44 papers →light · growth · Lactuca sativa (garden lettuce)Positive and negative results
242 papers in the same direction as this paper · 54 in the opposite direction · 3 reporting no difference from an alternative
- negative
- no effect
- no effect
296 papers more in this group
See all 300 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.