Abstract
In rapeseed, the oil content of the seed not only supplies energy for seed germination and seedling development but also provides essential dietary nutrients for humans and livestock. Recent studies have revealed that many transcription factors (TFs) regulate the accumulation of fatty acids (FAs) during seed development. WRKY6, a WRKY6 family TF, was reported to serve a function in the plant senescence processes, pathogen defense mechanisms and abiotic stress responses. However, the precise role of WRKY6 in influencing FA accumulation in seeds is still unknown. In this study, we demonstrate that WRKY6 has a high expression level in developing seeds and plays an essential role in regulating the accumulation of FAs in developing seeds of Arabidopsis. Mutation of WRKY6 resulted in significant increase in seed size, accompanied by an increase in FA content and changes in FA composition. Ultrastructure analyses showed that the absence of WRKY6 resulted in more and higher percentage of oil body in the cell of mature seeds. Quantitative real-time PCR analysis revealed changes in the expression of several genes related to photosynthesis and FA biosynthesis in wrky6 mutants at 10 or 16 days after pollination. These results reveal a novel function of WRKY6 influencing seed oil content and FAs compositions. This gene could be used as a promising gene resource to improve FA accumulation and seed yield in Brassica napus through genetic manipulation.
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Claims 9
Claim · model-written summary
WRKY6 has a high expression level in developing seeds.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Organ / stage
- developing seedse1
- Gene
- WRKY6e1
- Outcome
- high expression levele1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e1“WRKY6 has a high expression level in developing seeds and plays an essential role in regulating the accumulation of FAs in developing seeds of Arabidopsis”
Claim · model-written summary
WRKY6 plays an essential role in regulating the accumulation of fatty acids in developing seeds.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Organ / stage
- developing seedse1
- Gene
- WRKY6e1
- Outcome
- accumulation of FAse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e1“WRKY6 has a high expression level in developing seeds and plays an essential role in regulating the accumulation of FAs in developing seeds of Arabidopsis”
Claim · model-written summary
Mutation of WRKY6 significantly increased seed size.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- Mutation of WRKY6e2
- Gene
- WRKY6e2
- Outcome
- seed sizee2
- Direction
- increasee2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e2“Mutation of WRKY6 resulted in significant increase in seed size, accompanied by an increase in FA content and changes in FA composition”
Claim · model-written summary
Mutation of WRKY6 increased fatty acid content in seeds.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- Mutation of WRKY6e2
- Gene
- WRKY6e2
- Outcome
- FA contente2
- Direction
- increasee2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e2“Mutation of WRKY6 resulted in significant increase in seed size, accompanied by an increase in FA content and changes in FA composition”
Claim · model-written summary
Mutation of WRKY6 changed fatty acid composition.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- Mutation of WRKY6e2
- Gene
- WRKY6e2
- Outcome
- FA compositione2
- Direction
- changese2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e2“Mutation of WRKY6 resulted in significant increase in seed size, accompanied by an increase in FA content and changes in FA composition”
Claim · model-written summary
Absence of WRKY6 gave a higher percentage of oil bodies in the cells of mature seeds.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Organ / stage
- mature seedse3
- Treatment
- the absence of WRKY6e3
- Gene
- WRKY6e3
- Outcome
- percentage of oil bodye3
- Direction
- highere3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e3“Ultrastructure analyses showed that the absence of WRKY6 resulted in more and higher percentage of oil body in the cell of mature seeds”
Claim · model-written summary
In wrky6 mutants, expression of several genes related to photosynthesis and FA biosynthesis changed at 10 or 16 days after pollination.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Organ / stage
- at 10 or 16 days after pollinatione4
- Treatment
- wrky6 mutantse4
- Outcome
- expression of several genese4
- Direction
- changese4
- Qualifier
- related to photosynthesis and FA biosynthesise4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e4“Quantitative real-time PCR analysis revealed changes in the expression of several genes related to photosynthesis and FA biosynthesis in wrky6 mutants at 10 or 16 days after pollination”
Claim · model-written summary
WRKY6 influences seed oil content and fatty acid composition.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- WRKY6e5
- Outcome
- seed oil content and FAs compositionse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e5“These results reveal a novel function of WRKY6 influencing seed oil content and FAs compositions”
Claim · model-written summary
WRKY6 could be used to improve fatty acid accumulation and seed yield in Brassica napus.
- Taxon
- Brassica napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napuse6 - Outcome
- FA accumulation and seed yielde6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
t0“Arabidopsis thaliana”
e6“This gene could be used as a promising gene resource to improve FA accumulation and seed yield in Brassica napus through genetic manipulation”
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