Abstract
RPM1 is a CC-NBS-LRR protein that was first shown to be required for resistance to Pseudomonas syringae pv. maculicola in Arabidopsis thaliana. Our previous study showed that TaRPM1 gene in wheat was up-regulated about six times following infection by Puccinia striiformis f. sp. tritici (Pst) under high temperature, compared with normal temperature. To study the function of TaRPM1 in wheat high-temperature seedling-plant (HTSP) resistance to Pst, the full length of TaRPM1 was cloned, with three copies each located on chromosomes 1A, 1B and 1D. Transient expression of the TaRPM1-GFP fusion protein in Nicotiana benthamiana indicated that TaRPM1 localizes in the cytoplasm and nucleus. Profiling TaRPM1 expression indicated that TaRPM1 transcription was rapidly up-regulated upon Pst inoculation under high temperature. In addition, TaRPM1 was induced by exogenous salicylic acid hormone application. Silencing TaRPM1 in wheat cultivar Xiaoyan 6 (XY 6) resulted in reduced HTSP resistance to Pst in terms of reduced number of necrotic cells and increased uredinial length, whereas no obvious phenotypic changes were observed in TaRPM1-silenced leaves under normal temperature. Related defense genes TaPR1 and TaPR2 were down-regulated in TaRPM1-silenced plants under high temperature. We conclude that TaRPM1 is involved in HTSP resistance to Pst in XY 6.
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Claims 8
Claim · model-written summary
Three TaRPM1 copies were located on chromosomes 1A, 1B and 1D in wheat.
- Taxon
- Wheat
Triticum aestivumt0 - Gene
- TaRPM1e0
- Outcome
- three copies each located on chromosomes 1A, 1B and 1De0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the full length of TaRPM1 was cloned, with three copies each located on chromosomes 1A, 1B and 1D”
t0“Wheat”
Claim · model-written summary
TaRPM1 localized to the cytoplasm and nucleus in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Gene
- TaRPM1e0
- Outcome
- localizes in the cytoplasm and nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient expression of the TaRPM1-GFP fusion protein in Nicotiana benthamiana indicated that TaRPM1 localizes in the cytoplasm and nucleus.”
Claim · model-written summary
Pst inoculation at high temperature rapidly up-regulated TaRPM1 transcription in wheat.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- Pst inoculatione0
- Interactor
- Pste0pathogen
- Gene
- TaRPM1e0
- Outcome
- TaRPM1 transcriptione0
- Direction
- up-regulatede0
- Qualifier
- under high temperaturee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaRPM1 transcription was rapidly up-regulated upon Pst inoculation under high temperature”
t0“Wheat”
Claim · model-written summary
Exogenous salicylic acid application induced TaRPM1 in wheat.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- exogenous salicylic acid hormone applicatione0
- Gene
- TaRPM1e0
- Outcome
- TaRPM1e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaRPM1 was induced by exogenous salicylic acid hormone application”
t0“Wheat”
Claim · model-written summary
Silencing TaRPM1 in Xiaoyan 6 reduced high-temperature seedling resistance to Pst, with fewer necrotic cells.
- Taxon
- wheatTriticum aestivume0
- Material
- Xiaoyan 6e0
- Treatment
- Silencing TaRPM1e0
- Interactor
- Pste0pathogen
- Gene
- TaRPM1e0
- Outcome
- number of necrotic cellse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing TaRPM1 in wheat cultivar Xiaoyan 6 (XY 6) resulted in reduced HTSP resistance to Pst in terms of reduced number of necrotic cells and increased uredinial length”
Claim · model-written summary
Silencing TaRPM1 in Xiaoyan 6 increased Pst uredinial length at high temperature.
- Taxon
- wheatTriticum aestivume0
- Material
- Xiaoyan 6e0
- Treatment
- Silencing TaRPM1e0
- Interactor
- Pste0pathogen
- Gene
- TaRPM1e0
- Outcome
- uredinial lengthe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing TaRPM1 in wheat cultivar Xiaoyan 6 (XY 6) resulted in reduced HTSP resistance to Pst in terms of reduced number of necrotic cells and increased uredinial length”
Claim · model-written summary
TaRPM1-silenced wheat leaves showed no obvious phenotypic change at normal temperature.
- Taxon
- WheatTriticum aestivumt0
- Organ / stage
- leavese0
- Treatment
- TaRPM1-silencede0
- Gene
- TaRPM1e0
- Outcome
- phenotypic changese0
- Direction
- no obviouse0
- Qualifier
- under normal temperaturee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no obvious phenotypic changes were observed in TaRPM1-silenced leaves under normal temperature”
t0“Wheat”
Claim · model-written summary
TaPR1 and TaPR2 defense genes were down-regulated in TaRPM1-silenced wheat under high temperature.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- TaRPM1-silencede0
- Gene
- TaPR1 and TaPR2e0
- Outcome
- defense genes TaPR1 and TaPR2e0
- Direction
- down-regulatede0
- Qualifier
- under high temperaturee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Related defense genes TaPR1 and TaPR2 were down-regulated in TaRPM1-silenced plants under high temperature.”
t0“Wheat”
The same questions in other papers
inoculation · molecular: expression · Triticum aestivum (wheat)Positive and negative results
37 papers in the same direction as this paper · 9 in the opposite direction
- positive
- positive
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