Abstract
Abstract The cwp (cuticular water permeability) gene controls the development of cuticular microfissuring and subsequent fruit dehydration in tomato. The gene underwent silencing in the evolution of the fleshy cultivated tomato but is expressed in the primitive wild tomato relatives. The introgression of the expressed allele from the wild S. habrochaites ( cwp h ) into the cultivated tomato ( Solanum lycopersicum ) leads to the phenotype of fruit water loss during and following ripening. In this report, we show that low temperature impacts on the severity of the cuticular microfissure phenotype via a combination of effects on both expression and alternative splicing of cwp h . The cwp gene, comprising four exons and three introns, undergoes post-transcriptional alternative splicing processes, leading to seven alternative transcripts that differ in reading-frame lengths. Transgenic plants expressing each of the alternative transcripts identified the longest reading frame (VAR1) as the functional splice variant. Low temperature led to a strong upregulation of cwp h expression, compounded by an increase in the relative proportion of the functional VAR1 transcript, leading to increased severity of microfissuring of the cuticle. In summary, we demonstrate the molecular mechanism behind the horticultural phenomenon of the low-temperature effect on cuticular microfissures in the dehydrating tomato.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
Low temperature strongly upregulated cwp h expression in tomato.
- Taxon
- tomato
Solanum lycopersicume1 - Treatment
- Low temperaturee5
- Gene
- cwp he5
- Outcome
- cwp h expressione5
- Direction
- upregulatione5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The introgression of the expressed allele from the wild S. habrochaites ( cwp h ) into the cultivated tomato ( Solanum lycopersicum ) leads to the phenotype of fruit water loss during and following ripening”
e5“Low temperature led to a strong upregulation of cwp h expression, compounded by an increase in the relative proportion of the functional VAR1 transcript, leading to increased severity of microfissuring of the cuticle”
Claim · model-written summary
Low temperature increased the relative proportion of the functional VAR1 transcript in tomato.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- Low temperaturee5
- Gene
- VAR1e5
- Outcome
- relative proportion of the functional VAR1 transcripte5
- Direction
- increasee5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The introgression of the expressed allele from the wild S. habrochaites ( cwp h ) into the cultivated tomato ( Solanum lycopersicum ) leads to the phenotype of fruit water loss during and following ripening”
e5“Low temperature led to a strong upregulation of cwp h expression, compounded by an increase in the relative proportion of the functional VAR1 transcript, leading to increased severity of microfissuring of the cuticle”
Claim · model-written summary
Low temperature increased the severity of cuticular microfissuring in tomato fruit.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- Low temperaturee5
- Outcome
- severity of microfissuring of the cuticlee5
- Direction
- increasede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The introgression of the expressed allele from the wild S. habrochaites ( cwp h ) into the cultivated tomato ( Solanum lycopersicum ) leads to the phenotype of fruit water loss during and following ripening”
e5“Low temperature led to a strong upregulation of cwp h expression, compounded by an increase in the relative proportion of the functional VAR1 transcript, leading to increased severity of microfissuring of the cuticle”
Claim · model-written summary
The longest reading-frame transcript, VAR1, is the functional splice variant of cwp in tomato.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- VAR1e4
- Outcome
- functional splice variante4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The introgression of the expressed allele from the wild S. habrochaites ( cwp h ) into the cultivated tomato ( Solanum lycopersicum ) leads to the phenotype of fruit water loss during and following ripening”
e4“Transgenic plants expressing each of the alternative transcripts identified the longest reading frame (VAR1) as the functional splice variant”
Claim · model-written summary
The cwp gene undergoes alternative splicing and produces seven alternative transcripts differing in reading-frame length.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- cwp genee3
- Outcome
- alternative transcriptse3
- Value
- sevene3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The introgression of the expressed allele from the wild S. habrochaites ( cwp h ) into the cultivated tomato ( Solanum lycopersicum ) leads to the phenotype of fruit water loss during and following ripening”
e3“The cwp gene, comprising four exons and three introns, undergoes post-transcriptional alternative splicing processes, leading to seven alternative transcripts that differ in reading-frame lengths”
The same questions in other papers
cold · disease · Solanum lycopersicum (tomato)Positive and negative results
1 paper in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.