Abstract
Small post-translationally modified peptides are important signalling components of plant defence responses against phytopathogens, acting as both positive and negative modulators. PAMP-INDUCED SECRETED PEPTIDE (PIP) 1 and 2 have been shown to amplify plant immunity. Here we investigate the role of the related peptide PIP3 in the regulation of immune response in Arabidopsis. Treatment with synthetic PIP peptides led to similar transcriptome reprogramming, indicating an effect on innate immunity-related processes and phytohormones, including jasmonic acid (JA) biosynthesis and signalling. PIP3 overexpressing (OX) plants showed enhanced growth inhibition in response to flg22 exposure. In addition, flg22-induced production of reactive oxygen species and callose deposition was significantly reduced in PIP3-OX plants. Interestingly, PIP3-OX plants showed increased susceptibility toward both Botrytis cinerea and the biotrophic pathogen Pseudomonas syringae. Expression of both JA and salicylic acid (SA) biosynthesis and signalling genes was more induced during B. cinerea infection in PIP3-OX plants compared with wild-type plants. Promoter and ChIP-seq analyses indicated that the transcription factors WRKY18, WRKY33, and WRKY40 cooperatively act as repressors for PIP3. The results point to a fine-tuning role for PIP3 in modulation of immunity through the regulation of SA and JA biosynthesis and signalling pathways in Arabidopsis.
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Claims 8
Claim · model-written summary
Synthetic PIP peptide treatment led to similar transcriptome reprogramming in Arabidopsis.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Treatment
- synthetic PIP peptidese1
- Outcome
- transcriptome reprogramminge1
- Direction
- similare1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“modulates immunity in Arabidopsis thaliana”
e1“Treatment with synthetic PIP peptides led to similar transcriptome reprogramming”
Claim · model-written summary
PIP3 overexpressing plants showed enhanced growth inhibition in response to flg22 exposure.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- PIP3 overexpressing (OX) plantse3
- Treatment
- flg22 exposuree3
- Outcome
- growth inhibitione3
- Direction
- enhancede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“modulates immunity in Arabidopsis thaliana”
e3“PIP3 overexpressing (OX) plants showed enhanced growth inhibition in response to flg22 exposure”
Claim · model-written summary
flg22-induced production of reactive oxygen species and callose deposition was significantly reduced in PIP3-OX plants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- PIP3-OX plantse4
- Treatment
- flg22e4
- Outcome
- production of reactive oxygen species and callose depositione4
- Direction
- significantly reducede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“modulates immunity in Arabidopsis thaliana”
e4“flg22-induced production of reactive oxygen species and callose deposition was significantly reduced in PIP3-OX plants”
Claim · model-written summary
PIP3-OX plants were more susceptible to Botrytis cinerea.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- PIP3-OX plantse5
- Interactor
- Botrytis cinerea
Botrytis cinereae5pathogen - Outcome
- susceptibilitye5
- Direction
- increasede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“modulates immunity in Arabidopsis thaliana”
e5“PIP3-OX plants showed increased susceptibility toward both Botrytis cinerea and the biotrophic pathogen Pseudomonas syringae”
Claim · model-written summary
PIP3-OX plants were more susceptible to Pseudomonas syringae.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- PIP3-OX plantse5
- Interactor
- Pseudomonas syringae
Pseudomonas syringaee5pathogen - Outcome
- susceptibilitye5
- Direction
- increasede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“modulates immunity in Arabidopsis thaliana”
e5“PIP3-OX plants showed increased susceptibility toward both Botrytis cinerea and the biotrophic pathogen Pseudomonas syringae”
Claim · model-written summary
JA and SA biosynthesis and signalling genes were more induced during B. cinerea infection in PIP3-OX plants than in wild-type plants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- PIP3-OX plantse6
- Treatment
- B. cinerea infectione6
- Interactor
- B. cinereaBotrytis cinereae6pathogen
- Gene
- JA and salicylic acid (SA) biosynthesis and signalling genese6
- Outcome
- Expressione6
- Direction
- more inducede6
- Comparator
- wild-type plantse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“modulates immunity in Arabidopsis thaliana”
e6“Expression of both JA and salicylic acid (SA) biosynthesis and signalling genes was more induced during B. cinerea infection in PIP3-OX plants compared with wild-type plants”
Claim · model-written summary
WRKY18, WRKY33 and WRKY40 act as repressors of PIP3.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- WRKY18, WRKY33, and WRKY40e7
- Outcome
- act as repressors for PIP3e7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“modulates immunity in Arabidopsis thaliana”
e7“Promoter and ChIP-seq analyses indicated that the transcription factors WRKY18, WRKY33, and WRKY40 cooperatively act as repressors for PIP3”
Claim · model-written summary
PIP3 fine-tunes immunity through regulation of SA and JA biosynthesis and signalling in Arabidopsis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- modulation of immunitye2
- Qualifier
- regulation of SA and JA biosynthesis and signalling pathwayse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“modulates immunity in Arabidopsis thaliana”
e2“The results point to a fine-tuning role for PIP3 in modulation of immunity through the regulation of SA and JA biosynthesis and signalling pathways in Arabidopsis”
The same questions in other papers
resistance inducer · physiology: oxidative · Arabidopsis thaliana (thale cress)Positive and negative results
3 in the opposite direction
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.