Abstract
A mutant defective in the biosynthesis of thermospermine, acaulis5 (acl5), shows a dwarf phenotype with excess xylem vessels in Arabidopsis thaliana. Exogenous supply of thermospermine remarkably represses xylem differentiation in the root of seedlings, indicating the role of thermospermine in proper repression of xylem differentiation. However, the effect of thermospermine has rarely been investigated in other plant species. In this paper, we examined its effect on the growth and gene expression in rice seedlings. When grown with thermospermine, rice seedlings had no clearly enlarged metaxylem vessels in the root. Expression of OsACL5 was reduced in response to thermospermine, suggesting a negative feedback control of thermospermine biosynthesis like in Arabidopsis. Unlike Arabidopsis, however, rice showed up-regulation of phloem-expressed genes, OsHB5 and OsYSL16, by one-day treatment with thermospermine. Furthermore, expression of OsPAO2 and OsPAO6, encoding extracellular polyamine oxidase whose orthologs are not present in Arabidopsis, was induced by both thermospermine and spermine. These results suggest that thermospermine affects the expression of a subset of genes in rice different from those affected in Arabidopsis.
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Claims 5
Claim · model-written summary
Thermospermine-grown rice seedlings had no clearly enlarged root metaxylem vessels.
- Taxon
- rice seedlings
Oryza sativae0 - Organ / stage
- roote0
- Treatment
- thermosperminee0
- Outcome
- no clearly enlarged metaxylem vesselse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When grown with thermospermine, rice seedlings had no clearly enlarged metaxylem vessels in the root.”
Claim · model-written summary
Thermospermine reduced OsACL5 expression in rice seedlings.
- Taxon
- Rice SeedlingsOryza sativat0
- Treatment
- thermosperminee0
- Gene
- OsACL5e0
- Outcome
- Expression of OsACL5e0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of OsACL5 was reduced in response to thermospermine”
t0“Rice Seedlings”
Claim · model-written summary
A one-day thermospermine treatment up-regulated rice OsHB5 and OsYSL16 expression.
- Taxon
- riceOryza sativae0
- Treatment
- one-day treatment with thermosperminee0
- Gene
- OsHB5 and OsYSL16e0
- Outcome
- phloem-expressed genes, OsHB5 and OsYSL16e0
- Direction
- up-regulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice showed up-regulation of phloem-expressed genes, OsHB5 and OsYSL16, by one-day treatment with thermospermine”
Claim · model-written summary
Thermospermine induced rice OsPAO2 and OsPAO6 expression.
- Taxon
- Rice SeedlingsOryza sativat0
- Treatment
- thermosperminee0
- Gene
- OsPAO2 and OsPAO6e0
- Outcome
- expression of OsPAO2 and OsPAO6e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of OsPAO2 and OsPAO6, encoding extracellular polyamine oxidase whose orthologs are not present in Arabidopsis, was induced by both thermospermine and spermine”
t0“Rice Seedlings”
Claim · model-written summary
Spermine induced rice OsPAO2 and OsPAO6 expression.
- Taxon
- Rice SeedlingsOryza sativat0
- Treatment
- sperminee0
- Gene
- OsPAO2 and OsPAO6e0
- Outcome
- expression of OsPAO2 and OsPAO6e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of OsPAO2 and OsPAO6, encoding extracellular polyamine oxidase whose orthologs are not present in Arabidopsis, was induced by both thermospermine and spermine”
t0“Rice Seedlings”
The same questions in other papers
spermine · molecular: expression · Oryza sativa (rice)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.