Abstract
BACKGROUND: The WOX (WUSCHEL-RELATED HOMEOBOX) gene family encodes a class of transcription factors that are unique to green plants, where they are involved in regulating the development of plant tissues and organs by determining cell fate. Although the importance of the WOX gene is well known, there are few studies describing their functions in cotton. RESULTS: In this study, 32 WOX genes were found in Gossypium hirsutum. Phylogenetic analysis showed that WOX proteins of cotton can be divided into three clades: the ancient, intermediate, and WUS clades. The number of WOX proteins in the WUS clade was greater than the sum of the proteins in the other two clades. Our analysis revealed that 20 GhWOX genes are distributed on 16 cotton chromosomes and that duplication events are likely to have contributed to the expansion of the GhWOX family. All GhWOX genes have introns, and each GhWOX protein contains multiple motifs. RNA-seq data and real-time PCR showed that GhWOX13 gene subfamily is specifically expressed at a high level in cotton fibers. We also identified putative GA, NAA, and BR response elements in the promoter regions of the GhWOX13 genes and GhWOX13 transcripts were significantly induced by GA, NAA, and BR. CONCLUSIONS: Our data provides a useful resource for future studies on the functional roles of cotton WOX genes and shows that the GhWOX13 genes may influence cotton fiber development. Our results also provide an approach for identifying and characterizing WOX protein genes in other species.
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Claims 13
Claim · model-written summary
Gossypium hirsutum has 32 identified WOX genes.
- Taxon
- Gossypium hirsutum
Gossypium hirsutume0 - Gene
- WOX genese0
- Outcome
- 32 WOX genese0
- Value
- 32e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“32 WOX genes were found in Gossypium hirsutum”
Claim · model-written summary
Cotton WOX proteins fall into ancient, intermediate, and WUS clades.
- Taxon
- cottonGossypium hirsutume0
- Gene
- WOX proteinse0
- Outcome
- three clades: the ancient, intermediate, and WUS cladese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“WOX proteins of cotton can be divided into three clades: the ancient, intermediate, and WUS clades”
Claim · model-written summary
The WUS clade contains more WOX proteins than the other two clades combined.
- Taxon
- cottonGossypium hirsutumt0
- Gene
- WOX proteinse0
- Outcome
- number of WOX proteinse0
- Direction
- greatere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The number of WOX proteins in the WUS clade was greater than the sum of the proteins in the other two clades”
t0“cotton”
Claim · model-written summary
Twenty GhWOX genes are distributed across 16 cotton chromosomes.
- Taxon
- cottonGossypium hirsutume0
- Gene
- GhWOX genese0
- Outcome
- distributed on 16 cotton chromosomese0
- Value
- 20e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“20 GhWOX genes are distributed on 16 cotton chromosomes”
Claim · model-written summary
Duplication events likely contributed to expansion of the GhWOX family.
- Taxon
- cottonGossypium hirsutumt0
- Gene
- GhWOX familye0
- Outcome
- expansion of the GhWOX familye0
- Qualifier
- duplication eventse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“duplication events are likely to have contributed to the expansion of the GhWOX family”
t0“cotton”
Claim · model-written summary
All GhWOX genes have introns.
- Taxon
- cottonGossypium hirsutumt0
- Gene
- GhWOX genese0
- Outcome
- have intronse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All GhWOX genes have introns”
t0“cotton”
Claim · model-written summary
Each GhWOX protein contains multiple motifs.
- Taxon
- cottonGossypium hirsutumt0
- Gene
- GhWOX proteine0
- Outcome
- contains multiple motifse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“each GhWOX protein contains multiple motifs”
t0“cotton”
Claim · model-written summary
The GhWOX13 subfamily is expressed at a high level specifically in cotton fibers.
- Taxon
- cottonGossypium hirsutume0
- Organ / stage
- fiberse0
- Gene
- GhWOX13 gene subfamilye0
- Outcome
- expressed at a high levele0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhWOX13 gene subfamily is specifically expressed at a high level in cotton fibers”
Claim · model-written summary
Promoters of GhWOX13 genes contain putative GA, NAA, and BR response elements.
- Taxon
- cottonGossypium hirsutumt0
- Gene
- GhWOX13 genese0
- Outcome
- putative GA, NAA, and BR response elementse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“putative GA, NAA, and BR response elements in the promoter regions of the GhWOX13 genes”
t0“cotton”
Claim · model-written summary
GA significantly induced GhWOX13 transcripts.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- GAe0
- Gene
- GhWOX13 transcriptse0
- Outcome
- GhWOX13 transcriptse0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhWOX13 transcripts were significantly induced by GA”
t0“cotton”
Claim · model-written summary
NAA significantly induced GhWOX13 transcripts.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- NAAe0
- Gene
- GhWOX13 transcriptse0
- Outcome
- GhWOX13 transcriptse0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhWOX13 transcripts were significantly induced by GA, NAA, and BR”
t0“cotton”
Claim · model-written summary
BR significantly induced GhWOX13 transcripts.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- BRe0
- Gene
- GhWOX13 transcriptse0
- Outcome
- GhWOX13 transcriptse0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhWOX13 transcripts were significantly induced by GA, NAA, and BR”
t0“cotton”
Claim · model-written summary
The GhWOX13 genes may influence cotton fiber development.
- Taxon
- cottonGossypium hirsutume0
- Organ / stage
- fibere0
- Gene
- GhWOX13 genese0
- Outcome
- cotton fiber developmente0
- Qualifier
- maye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhWOX13 genes may influence cotton fiber development”
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