Abstract
Abstract Cadmium (Cd) is highly toxic to living organisms. This study aimed to elucidate the regulation of gene expression in rice shoots under Cd stress. Rice plants were exposed to 0, 50, 75, 100 μmol/L CdCl 2 in hydroponic culture for 7 d. Transcriptional changes in rice shoots were examined by transcriptome sequencing techniques. A total of 2197 DEGs (987 up-regulated and 1210 down-regulated) were detected in rice shoots under the exposure of 75 μmol/L CdCl 2 . GO and KEGG enrichment analyses showed that genes encoding auxin-responsive protein IAA and peroxidase were up-regulated, while genes encoding proteins involved in signal transduction, including TIFY family, ERF and bZIP were down-regulated. Abundant ROS related terms were also identified and grouped into significantly differentially expressed GO terms, including oxidoreductase activity, catalytic activity, oxidation-reduction process, confirming the enhanced oxidative stress of Cd. Genes encoding photosystem I reaction center subunit and photosynthetic NDH subunit of luminal location were up-regulated in pathway of energy metabolism, suggesting an interference of photosynthesis by Cd stress. Our results improve the understanding of the complex molecular responsive mechanisms of rice shoots under Cd stress.
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Claims 9
Claim · model-written summary
In rice shoots exposed to 75 μmol/L CdCl2, 2197 DEGs were detected.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 2197 DEGs (987 up-regulated and 1210 down-regulated) were detected in rice shoots under the exposure of 75 μmol/L CdCl 2”
Claim · model-written summary
Of the DEGs in rice shoots under 75 μmol/L CdCl2, 987 were up-regulated.
- Taxon
- rice
Oryza sativae0 - Organ / stage
- shootse0
- Treatment
- 75 μmol/L CdCl 2e0
- Outcome
- DEGse0
- Direction
- up-regulatede0
- Value
- 987e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 2197 DEGs (987 up-regulated and 1210 down-regulated) were detected in rice shoots under the exposure of 75 μmol/L CdCl 2”
Claim · model-written summary
Of the DEGs in rice shoots under 75 μmol/L CdCl2, 1210 were down-regulated.
- Taxon
- riceOryza sativae0
- Organ / stage
- shootse0
- Treatment
- 75 μmol/L CdCl 2e0
- Outcome
- DEGse0
- Direction
- down-regulatede0
- Value
- 1210e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 2197 DEGs (987 up-regulated and 1210 down-regulated) were detected in rice shoots under the exposure of 75 μmol/L CdCl 2”
Claim · model-written summary
Genes encoding auxin-responsive protein IAA and peroxidase were up-regulated in rice shoots under cadmium stress.
- Taxon
- riceOryza sativat0
- Treatment
- cadmium stresst0
- Outcome
- genes encoding auxin-responsive protein IAA and peroxidasee0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genes encoding auxin-responsive protein IAA and peroxidase were up-regulated”
t0“rice (Oryza sativa L.) shoots responsive to cadmium stress”
Claim · model-written summary
Genes encoding signal-transduction proteins, including TIFY family, ERF and bZIP, were down-regulated in rice shoots under cadmium stress.
- Taxon
- riceOryza sativat0
- Treatment
- cadmium stresst0
- Outcome
- genes encoding proteins involved in signal transduction, including TIFY family, ERF and bZIPe0
- Direction
- down-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genes encoding proteins involved in signal transduction, including TIFY family, ERF and bZIP were down-regulated”
t0“rice (Oryza sativa L.) shoots responsive to cadmium stress”
Claim · model-written summary
Abundant ROS-related terms were identified and grouped into significantly differentially expressed GO terms.
- Taxon
- riceOryza sativat0
- Outcome
- Abundant ROS related termse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Abundant ROS related terms were also identified and grouped into significantly differentially expressed GO terms”
t0“rice (Oryza sativa L.) shoots responsive to cadmium stress”
Claim · model-written summary
The authors interpret the ROS-related GO terms as confirming enhanced oxidative stress caused by Cd.
- Taxon
- riceOryza sativat0
- Treatment
- Cde0
- Outcome
- oxidative stresse0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“confirming the enhanced oxidative stress of Cd”
t0“rice (Oryza sativa L.) shoots responsive to cadmium stress”
Claim · model-written summary
Genes encoding photosystem I reaction center subunit and photosynthetic NDH subunit of luminal location were up-regulated in the energy metabolism pathway under Cd stress.
- Taxon
- riceOryza sativat0
- Treatment
- cadmium stresst0
- Outcome
- Genes encoding photosystem I reaction center subunit and photosynthetic NDH subunit of luminal locatione0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Genes encoding photosystem I reaction center subunit and photosynthetic NDH subunit of luminal location were up-regulated in pathway of energy metabolism”
t0“rice (Oryza sativa L.) shoots responsive to cadmium stress”
Claim · model-written summary
The authors suggest Cd stress interferes with photosynthesis.
- Taxon
- riceOryza sativat0
- Treatment
- Cd stresse0
- Outcome
- photosynthesise0
- Direction
- interferencee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggesting an interference of photosynthesis by Cd stress”
t0“rice (Oryza sativa L.) shoots responsive to cadmium stress”
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