Abstract
Here we highlight the development of a simple and high-throughput mung bean model to study virulence in the opportunistic pathogen Pseudomonas aeruginosa. The model is easy to set up, and infection and virulence can be monitored for up to 10 days. In a first test of the model, we found that mung bean seedlings infected with PAO1 showed poor development of roots and high mortality rates compared to uninfected controls. We also found that a quorum-sensing (QS) mutant was significantly less virulent when compared with the PAO1 wild-type. Our work introduces a new tool for studying virulence in P. aeruginosa that will allow for high-throughput virulence studies of mutants and testing of the in vivo efficacy of new therapies at a time when new antimicrobial drugs are desperately needed.
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Claims 4
Claim · model-written summary
The mung bean model permits infection and virulence monitoring for up to 10 days.
- Taxon
- mung bean
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Vigna radiatat0 - Outcome
- infection and virulencee1
- Value
- up to 10 dayse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e1“infection and virulence can be monitored for up to 10 days”
t0“mung bean”
Claim · model-written summary
PAO1-infected mung bean seedlings showed poorer root development than uninfected controls.
- Taxon
- mung bean seedlingsVigna radiatae1
- Treatment
- infected with PAO1e1
- Interactor
- PAO1e1pathogen
- Outcome
- development of rootse1
- Direction
- poore1
- Comparator
- uninfected controlse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“mung bean seedlings infected with PAO1 showed poor development of roots and high mortality rates compared to uninfected controls”
Claim · model-written summary
PAO1-infected mung bean seedlings had high mortality relative to uninfected controls.
- Taxon
- mung bean seedlingsVigna radiatae1
- Treatment
- infected with PAO1e1
- Interactor
- PAO1e1pathogen
- Outcome
- mortality ratese1
- Direction
- highe1
- Comparator
- uninfected controlse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“mung bean seedlings infected with PAO1 showed poor development of roots and high mortality rates compared to uninfected controls”
Claim · model-written summary
A quorum-sensing mutant was significantly less virulent on mung bean than wild-type PAO1.
- Taxon
- mung beanVigna radiatat0
- Treatment
- quorum-sensing (QS) mutante1
- Interactor
- quorum-sensing (QS) mutante1pathogen
- Outcome
- virulente1
- Direction
- lesse1
- Comparator
- PAO1 wild-typee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a quorum-sensing (QS) mutant was significantly less virulent when compared with the PAO1 wild-type”
t0“mung bean”
The same questions in other papers
infection · growth · Vigna radiata (mung bean)Positive and negative results
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- positive
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See all 7 papers →infection · pest · Vigna radiata (mung bean)Results agree
2 papers in the same direction as this paper
- negative
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.