Abstract
ABSTRACT: The objective of this research was to evaluate the proline synthesis and physiological response of cassava genotypes which were micro propagated and induced to salinity stress in vitro. Micro cuttings of approximately 1.0cm long with a single bud of genotypes TBRS Tapioqueira, BRS Verdinha and Lagoão which were previously established in vitro were inoculated in a MS medium containing different concentrations of NaCl (0; 25; 50; 75; 100mM) and were analyzed after 90th day for: number of roots, number of leaves and shoot dry mass. The proline content of BRS Tapioqueira and Lagoão was assessed at 30th, 60th and 90th day. There was no analysis of proline of the variety Verdinha because of the contamination of the explants. The experimental design was completely randomized in double factorial scheme (3 genotypes x 5 salt treatments), with seven repetitions for growth variables. For comparing proline content, completely randomized design was used in a plot subdivided in time, with genotype and NaCl factors in plot and time in subplot, with two repetitions. For r time and genotypes Tukey test (P<0,05) was used and for NaCl levels regression test (P<0,05). Salinity affected the growth of all varieties; although, BRS Tapioqueira and BRS Verdinha were less affected by induced salt stress. There was an increase in the accumulation of proline from the salt increment, this synthesis of proline being a biochemical indicator of salt stress in cassava plants cultivated in vitro.
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Claims 3
Claim · model-written summary
Salinity affected growth in all cassava varieties.
- Taxon
- cassava
Manihot esculentat0 - Treatment
- Salinitye0
- Outcome
- growth of all varietiese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Salinity affected the growth of all varieties”
t0“cassava”
Claim · model-written summary
BRS Tapioqueira and BRS Verdinha were less affected by salt stress.
- Taxon
- cassavaManihot esculentat0
- Material
- BRS Tapioqueira and BRS Verdinhae0
- Outcome
- less affected by induced salt stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BRS Tapioqueira and BRS Verdinha were less affected by induced salt stress”
t0“cassava”
Claim · model-written summary
Proline accumulation increased with increasing salt.
- Taxon
- cassavaManihot esculentat0
- Treatment
- salt incremente0
- Outcome
- accumulation of prolinee0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There was an increase in the accumulation of proline from the salt increment”
t0“cassava”
The same questions in other papers
salinity · growth · Manihot esculenta (cassava)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.