Abstract
Adequate phosphorous (P) is essential to plant cells to ensure normal plant growth and development. Therefore, plants employ elegant mechanisms to regulate P abundance across their developmentally distinct tissues. One such mechanism is PHOSPHATE2 (PHO2)-directed ubiquitin-mediated degradation of a cohort of phosphate (PO4) transporters. PHO2 is itself under tight regulation by the PO4 responsive microRNA (miRNA), miR399. The DOUBLE-STRANDED RNA BINDING (DRB) proteins, DRB1, DRB2 and DRB4, have each been assigned a specific functional role in the Arabidopsis thaliana (Arabidopsis) miRNA pathway. Here, we assessed the requirement of DRB1, DRB2 and DRB4 to regulate the miR399/PHO2 expression module under PO4 starvations conditions. Via the phenotypic and molecular assessment of the knockout mutant plant lines, drb1, drb2 and drb4, we show here that; (1) DRB1 and DRB2 are required to maintain P homeostasis in Arabidopsis shoot and root tissues; (2) DRB1 is the primary DRB required for miR399 production; (3) DRB2 and DRB4 play secondary roles in regulating miR399 production, and; (4) miR399 appears to direct expression regulation of the PHO2 transcript via both an mRNA cleavage and translational repression mode of RNA silencing. Together, the hierarchical contribution of DRB1, DRB2 and DRB4 demonstrated here to be required for the appropriate regulation of the miR399/PHO2 expression module identifies the extreme importance of P homeostasis maintenance in Arabidopsis to ensure that numerous vital cellular processes are maintained across Arabidopsis tissues under a changing cellular environment.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 4
Claim · model-written summary
DRB1 and DRB2 are required to maintain P homeostasis in Arabidopsis shoot and root tissues.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Organ / stage
- shoot and root tissuese1
- Treatment
- knockout mutant plant linese5
- Gene
- DRB1 and DRB2e1
- Outcome
- P homeostasise1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e1“DRB1 and DRB2 are required to maintain P homeostasis in Arabidopsis shoot and root tissues”
e5“knockout mutant plant lines, drb1, drb2 and drb4”
Claim · model-written summary
DRB1 is the primary DRB required for miR399 production.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- knockout mutant plant linese5
- Gene
- DRB1e2
- Outcome
- miR399 productione2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e2“DRB1 is the primary DRB required for miR399 production”
e5“knockout mutant plant lines, drb1, drb2 and drb4”
Claim · model-written summary
DRB2 and DRB4 play secondary roles in regulating miR399 production.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- knockout mutant plant linese5
- Gene
- DRB2 and DRB4e3
- Outcome
- miR399 productione3
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e3“DRB2 and DRB4 play secondary roles in regulating miR399 production”
e5“knockout mutant plant lines, drb1, drb2 and drb4”
Claim · model-written summary
miR399 appears to direct expression regulation of the PHO2 transcript via both mRNA cleavage and translational repression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- PHO2e4
- Outcome
- expression regulation of the PHO2 transcripte4
- Qualifier
- mRNA cleavage and translational repressione4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e4“miR399 appears to direct expression regulation of the PHO2 transcript via both an mRNA cleavage and translational repression mode of RNA silencing”
The same questions in other papers
loss of function · yield · Arabidopsis thaliana (thale cress)Positive and negative results
4 papers in the same direction as this paper · 13 in the opposite direction
- no effect
- no effect
- negative
14 papers more in this group
See all 18 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.