Abstract
Tomato spotted wilt virus (TSWV) causes major losses of many crops worldwide. Several strategies have been attempted to control disease caused by TSWV. However, many challenges for the effective control of this disease remain. A promising approach is the use of abiotic or biotic inducers to enhance plant resistance to pathogens. We screened a diterpenoid compound from Wedelia trilobata, 3α-Angeloyloxy-9β-hydroxy-ent-kaur-16-en-19-oic acid (AHK), which had higher curative and protective effects against TSWV than the ningnanmycin control. The rapid initiation of the expression of all the TSWV genes was delayed by more than 1d in the curative assay, and the expression of the NSs, NSm and RdRp genes was inhibited. In addition, the replication of all TSWV genes in systemic leaves was inhibited in the protective assay, with an inhibition rate of more than 90%. The concentrations of jasmonic acid (JA) and jasmonic acid isoleucine (JA-ILE) in the AHK-treated and systemic leaves of the treated plants were significantly higher than those observed in the control. The results suggested that AHK can induce systemic resistance in treated plants. The transcription of the NtCOI1 gene, a key gene in the JA pathway, was significantly higher in both the inoculated and systemic leaves of the AHK-treated plants compared to the control. The AHK-induced resistance to TSWV in Nicotiana benthamiana could be eliminated by VIGS-mediated silencing of the NtCOI1 gene. These results indicated that AHK can activate the JA pathway and induce systemic resistance to TSWV infection.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
AHK had greater curative effects against TSWV than ningnanmycin.
- Taxon
- tobacco plants
Nicotiana tabacumt0 - Treatment
- AHKe0
- Interactor
- TSWV
Orthotospovirus tomatomaculaee0pathogen - Outcome
- curativee0
- Direction
- highere0
- Comparator
- ningnanmycin controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“3α-Angeloyloxy-9β-hydroxy-ent-kaur-16-en-19-oic acid (AHK), which had higher curative and protective effects against TSWV than the ningnanmycin control”
t0“tobacco plants”
Claim · model-written summary
AHK had greater protective effects against TSWV than ningnanmycin.
- Taxon
- tobacco plantsNicotiana tabacumt0
- Treatment
- AHKe0
- Interactor
- TSWVOrthotospovirus tomatomaculaee0pathogen
- Outcome
- protective effectse0
- Direction
- highere0
- Comparator
- ningnanmycin controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“3α-Angeloyloxy-9β-hydroxy-ent-kaur-16-en-19-oic acid (AHK), which had higher curative and protective effects against TSWV than the ningnanmycin control”
t0“tobacco plants”
Claim · model-written summary
AHK delayed initiation of expression of all TSWV genes by over one day in the curative assay.
- Taxon
- tobacco plantsNicotiana tabacumt0
- Treatment
- AHKe1
- Interactor
- TSWVOrthotospovirus tomatomaculaee0pathogen
- Outcome
- expression of all the TSWV genese0
- Direction
- delayede0
- Value
- more than 1de0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The rapid initiation of the expression of all the TSWV genes was delayed by more than 1d in the curative assay”
e1“acid (AHK)”
t0“tobacco plants”
Claim · model-written summary
AHK inhibited expression of NSs, NSm and RdRp viral genes.
- Taxon
- tobacco plantsNicotiana tabacumt0
- Treatment
- AHKe1
- Interactor
- TSWVOrthotospovirus tomatomaculaee2pathogen
- Outcome
- expression of the NSs, NSm and RdRp genese0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of the NSs, NSm and RdRp genes was inhibited”
e1“acid (AHK)”
e2“all the TSWV genes was delayed”
t0“tobacco plants”
Claim · model-written summary
AHK inhibited replication of all TSWV genes in systemic leaves by over 90%.
- Taxon
- tobacco plantsNicotiana tabacumt0
- Organ / stage
- systemic leavese0
- Treatment
- AHKe1
- Interactor
- TSWVOrthotospovirus tomatomaculaee0pathogen
- Outcome
- replication of all TSWV genese0
- Direction
- inhibitede0
- Value
- more than 90%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the replication of all TSWV genes in systemic leaves was inhibited in the protective assay, with an inhibition rate of more than 90%”
e1“acid (AHK)”
t0“tobacco plants”
Claim · model-written summary
AHK increased jasmonic acid concentrations in treated and systemic leaves.
- Taxon
- tobacco plantsNicotiana tabacumt0
- Organ / stage
- systemic leavese0
- Treatment
- AHK-treatede0
- Outcome
- concentrations of jasmonic acid (JA)e0
- Direction
- highere0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The concentrations of jasmonic acid (JA) and jasmonic acid isoleucine (JA-ILE) in the AHK-treated and systemic leaves of the treated plants were significantly higher than those observed in the control”
t0“tobacco plants”
Claim · model-written summary
AHK increased jasmonic acid isoleucine concentrations in treated and systemic leaves.
- Taxon
- tobacco plantsNicotiana tabacumt0
- Organ / stage
- systemic leavese0
- Treatment
- AHK-treatede0
- Outcome
- jasmonic acid isoleucine (JA-ILE)e0
- Direction
- highere0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The concentrations of jasmonic acid (JA) and jasmonic acid isoleucine (JA-ILE) in the AHK-treated and systemic leaves of the treated plants were significantly higher than those observed in the control”
t0“tobacco plants”
Claim · model-written summary
AHK was inferred to induce systemic resistance.
- Taxon
- tobacco plantsNicotiana tabacumt0
- Treatment
- AHKe0
- Outcome
- systemic resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results suggested that AHK can induce systemic resistance in treated plants”
t0“tobacco plants”
Claim · model-written summary
AHK significantly increased NtCOI1 transcription in inoculated and systemic leaves.
- Taxon
- tobacco plantsNicotiana tabacumt0
- Organ / stage
- inoculated and systemic leavese0
- Treatment
- AHK-treatede0
- Gene
- NtCOI1e0
- Outcome
- transcription of the NtCOI1 genee0
- Direction
- highere0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcription of the NtCOI1 gene, a key gene in the JA pathway, was significantly higher in both the inoculated and systemic leaves of the AHK-treated plants compared to the control”
t0“tobacco plants”
Claim · model-written summary
Silencing NtCOI1 eliminated AHK-induced TSWV resistance in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- VIGS-mediated silencing of the NtCOI1 genee0
- Interactor
- TSWVOrthotospovirus tomatomaculaee0pathogen
- Gene
- NtCOI1e0
- Outcome
- AHK-induced resistance to TSWVe0
- Direction
- eliminatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The AHK-induced resistance to TSWV in Nicotiana benthamiana could be eliminated by VIGS-mediated silencing of the NtCOI1 gene”
The same questions in other papers
loss of function · Orthotospovirus tomatomaculae · Nicotiana benthamianaPositive and negative results
4 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- negative
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.