Abstract
Potassium (K) deficiency is a key limiting factor in cotton (Gossypium hirsutum) production. By grafting two contrasting cotton cultivars, CCRI41 (more susceptible to K+ deficiency) and SCRC22 (more tolerant of K+ deficiency), we established that cotton shoot plays a vital role in the regulation of root K+ uptake. To identify the genetic basis of this finding, we performed RNA sequencing (RNA-seq) of roots of CCRI41 self-grafts (CCRI41/CCRI41, scion/rootstock) and SCRC22/CCRI41 reciprocal-grafts exposed to K+ deficiency. We found that GhHAK5a, an orthologous of Arabidopsis thaliana high-affinity K+ transporter, AtHAK5, was significantly induced in the CCRI41 rootstock by the SCRC22 scion. This gene was mainly expressed in roots and was more highly induced by K+ deficiency in roots of SCRC22 than those of CCRI41. Agrobacterium-mediated virus-induced gene silencing and yeast complementary assay showed that GhHAK5a is a high-affinity K+ uptake transporter. Importantly, silencing of GhHAK5a in the CCRI41 rootstock almost completely inhibited the K+ uptake induced by SCRC22 scion in CCRI41 rootstock. We identified a key high-affinity K+ transporter, GhHAK5a in cotton, which is the essential target for shoot regulation of root K+ uptake under K+ deficiency.
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Claims 7
Claim · model-written summary
Cotton shoots play a vital role in regulating root potassium uptake.
- Taxon
- cotton
Gossypium hirsutume0 - Organ / stage
- shoote0
- Outcome
- root K+ uptakee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we established that cotton shoot plays a vital role in the regulation of root K+ uptake.”
Claim · model-written summary
The SCRC22 scion significantly induced GhHAK5a in the CCRI41 rootstock.
- Taxon
- CottonGossypium hirsutumt0
- Material
- CCRI41e0
- Organ / stage
- rootstocke0
- Treatment
- SCRC22 scione0
- Gene
- GhHAK5ae0
- Outcome
- GhHAK5ae0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We found that GhHAK5a, an orthologous of Arabidopsis thaliana high-affinity K+ transporter, AtHAK5, was significantly induced in the CCRI41 rootstock by the SCRC22 scion.”
t0“Cotton”
Claim · model-written summary
GhHAK5a was mainly expressed in roots.
- Taxon
- CottonGossypium hirsutumt0
- Organ / stage
- rootse0
- Gene
- GhHAK5at1
- Outcome
- mainly expressed in rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This gene was mainly expressed in roots”
t0“Cotton”
t1“GhHAK5a”
Claim · model-written summary
Potassium deficiency induced GhHAK5a more highly in SCRC22 roots than CCRI41 roots.
- Taxon
- CottonGossypium hirsutumt0
- Material
- SCRC22e0
- Organ / stage
- rootse0
- Treatment
- K+ deficiencye0
- Gene
- GhHAK5at1
- Outcome
- GhHAK5at1
- Direction
- more highly inducede0
- Comparator
- CCRI41e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was more highly induced by K+ deficiency in roots of SCRC22 than those of CCRI41.”
t0“Cotton”
t1“GhHAK5a”
Claim · model-written summary
GhHAK5a is a high-affinity potassium uptake transporter.
- Taxon
- CottonGossypium hirsutumt0
- Gene
- GhHAK5ae0
- Outcome
- high-affinity K+ uptake transportere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Agrobacterium-mediated virus-induced gene silencing and yeast complementary assay showed that GhHAK5a is a high-affinity K+ uptake transporter.”
t0“Cotton”
Claim · model-written summary
Silencing GhHAK5a almost completely inhibited SCRC22-scion-induced potassium uptake in CCRI41 rootstocks.
- Taxon
- CottonGossypium hirsutumt0
- Material
- CCRI41e0
- Organ / stage
- rootstocke0
- Treatment
- silencing of GhHAK5ae0
- Gene
- GhHAK5ae0
- Outcome
- K+ uptakee0
- Direction
- inhibitede0
- Qualifier
- almost completelye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of GhHAK5a in the CCRI41 rootstock almost completely inhibited the K+ uptake induced by SCRC22 scion in CCRI41 rootstock.”
t0“Cotton”
Claim · model-written summary
GhHAK5a is an essential target of shoot regulation of root potassium uptake under deficiency.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- K+ deficiencye0
- Gene
- GhHAK5ae0
- Outcome
- shoot regulation of root K+ uptakee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We identified a key high-affinity K+ transporter, GhHAK5a in cotton, which is the essential target for shoot regulation of root K+ uptake under K+ deficiency.”
The same questions in other papers
loss of function · physiology: ion balance · Gossypium hirsutum (cotton)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- negative
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.