Abstract
DNA methylation is an epigenetic modification involving many biological processes. It is known that epigenetic mechanisms such as cytosine methylation play a pivotal role in regulating plant development. In this current study, a conservative domain-spliced hairpin RNA (ihpRNA) plant expression vector aimed at gene of DNA METHYLTRANSFERASE1 ( CmMET1 ) has been constructed. Transgenic chrysanthemum materials (Zijingling) were obtained by Agrobacterium -mediated transformation with expression vectors. Transgenic plants were used as rootstock, grafted onto non-transgenic the scions [Guoqinghong (GQH) and Huanshuijinqiu (HSJQ)], which silencing CmMET1 gene, and exhibited the early flower phenotypes. A high-performance liquid chromatography analysis indicated that the decrease of CmMET1 expression decreased the methylation level of genomic DNA. Similarly, a quantitative real-time polymerase chain reaction analysis revealed that CmMET1 expression levels decreased in transgenic chrysanthemum plants and in the scions grafted onto transgenic plants. This decrease of CmMET1 expression upregulated the expression of the methyltransferase gene, METHYLTRANSFERASE2 ( CmDRM2 ), but downregulated the expression of the demethylating enzyme gene, DEMETER ( CmDME ), while the CHROMOMETHYLASEA ( CmCMT3 ) expression level remained low and could be almost undetectable. Among the CmFT-like s genes that affect flowering time, CmFTL1 expression was downregulated, as well as CmFTL2 and CmFTL3 expression levels which were upregulated. Our data indicated that silencing CmMET1 could decrease plant height, change the phenotype of chrysanthemum, and promote earlier flowering. The transgenic plants bloomed 8 days earlier. GQH and HSJQ scions grafted onto transgenic plants were 12 and 9 days earlier than the scions grafted onto non-transgenic plants, respectively. Overall, the results have some meanings for promoting the flowering of chrysanthemum scion varieties using genetic modified rootstock.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 15
Claim · model-written summary
Transgenic rootstocks gave grafted scions early flowering.
- Taxon
- chrysanthemum
.jpeg?width=60)
Chrysanthemumt0 - Treatment
- Transgenic plants were used as rootstocke0
- Gene
- CmMET1e0
- Outcome
- early flower phenotypese0
- Direction
- earlye0
- Comparator
- non-transgenice0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpeg?width=500)
Verbatim
e0“Transgenic plants were used as rootstock, grafted onto non-transgenic the scions [Guoqinghong (GQH) and Huanshuijinqiu (HSJQ)], which silencing CmMET1 gene, and exhibited the early flower phenotypes.”
t0“chrysanthemum (Chrysanthemum morifolium)”
Claim · model-written summary
Reduced CmMET1 expression decreased genomic DNA methylation.
- Taxon
- chrysanthemumChrysanthemumt0
- Gene
- CmMET1e0
- Outcome
- methylation level of genomic DNAe0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the decrease of CmMET1 expression decreased the methylation level of genomic DNA.”
t0“chrysanthemum (Chrysanthemum morifolium)”
Claim · model-written summary
CmMET1 expression fell in transgenic chrysanthemum plants.
- Taxon
- chrysanthemumChrysanthemumt0
- Treatment
- transgenic chrysanthemum plantse0
- Gene
- CmMET1e0
- Outcome
- CmMET1 expression levelse0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CmMET1 expression levels decreased in transgenic chrysanthemum plants and in the scions grafted onto transgenic plants.”
t0“chrysanthemum (Chrysanthemum morifolium)”
Claim · model-written summary
CmMET1 expression fell in scions grafted to transgenic plants.
- Taxon
- chrysanthemumChrysanthemumt0
- Treatment
- scions grafted onto transgenic plantse0
- Gene
- CmMET1e0
- Outcome
- CmMET1 expression levelse0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CmMET1 expression levels decreased in transgenic chrysanthemum plants and in the scions grafted onto transgenic plants.”
t0“chrysanthemum (Chrysanthemum morifolium)”
Claim · model-written summary
Reduced CmMET1 expression upregulated CmDRM2.
- Taxon
- chrysanthemumChrysanthemumt0
- Gene
- CmDRM2e0
- Outcome
- expression of the methyltransferase gene, METHYLTRANSFERASE2 ( CmDRM2 )e0
- Direction
- upregulatede0
- Qualifier
- decrease of CmMET1 expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This decrease of CmMET1 expression upregulated the expression of the methyltransferase gene, METHYLTRANSFERASE2 ( CmDRM2 )”
t0“chrysanthemum (Chrysanthemum morifolium)”
Claim · model-written summary
Reduced CmMET1 expression downregulated CmDME.
- Taxon
- chrysanthemumChrysanthemumt0
- Gene
- CmDMEe0
- Outcome
- expression of the demethylating enzyme gene, DEMETER ( CmDME )e0
- Direction
- downregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“downregulated the expression of the demethylating enzyme gene, DEMETER ( CmDME )”
t0“chrysanthemum (Chrysanthemum morifolium)”
Claim · model-written summary
CmCMT3 expression remained low and nearly undetectable.
- Taxon
- chrysanthemumChrysanthemumt0
- Gene
- CmCMT3e0
- Outcome
- expression levele0
- Direction
- remained lowe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the CHROMOMETHYLASEA ( CmCMT3 ) expression level remained low and could be almost undetectable.”
t0“chrysanthemum (Chrysanthemum morifolium)”
Claim · model-written summary
CmFTL1 expression was downregulated.
- Taxon
- chrysanthemumChrysanthemumt0
- Gene
- CmFTL1e0
- Outcome
- CmFTL1 expressione0
- Direction
- downregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CmFTL1 expression was downregulated”
t0“chrysanthemum (Chrysanthemum morifolium)”
Claim · model-written summary
CmFTL2 and CmFTL3 expression was upregulated.
- Taxon
- chrysanthemumChrysanthemumt0
- Gene
- CmFTL2 and CmFTL3e0
- Outcome
- CmFTL2 and CmFTL3 expression levelse0
- Direction
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CmFTL2 and CmFTL3 expression levels which were upregulated.”
t0“chrysanthemum (Chrysanthemum morifolium)”
Claim · model-written summary
Silencing CmMET1 decreased plant height.
- Taxon
- chrysanthemumChrysanthemumt0
- Treatment
- silencing CmMET1e0
- Gene
- CmMET1e0
- Outcome
- plant heighte0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing CmMET1 could decrease plant height”
t0“chrysanthemum (Chrysanthemum morifolium)”
Claim · model-written summary
Silencing CmMET1 altered chrysanthemum phenotype.
- Taxon
- chrysanthemumChrysanthemumt0
- Treatment
- silencing CmMET1e0
- Gene
- CmMET1e0
- Outcome
- phenotype of chrysanthemume0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing CmMET1 could decrease plant height, change the phenotype of chrysanthemum, and promote earlier flowering.”
t0“chrysanthemum (Chrysanthemum morifolium)”
Claim · model-written summary
Silencing CmMET1 promoted earlier flowering.
- Taxon
- chrysanthemumChrysanthemumt0
- Treatment
- silencing CmMET1e0
- Gene
- CmMET1e0
- Outcome
- floweringe0
- Direction
- earliere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing CmMET1 could decrease plant height, change the phenotype of chrysanthemum, and promote earlier flowering.”
t0“chrysanthemum (Chrysanthemum morifolium)”
Claim · model-written summary
Transgenic plants flowered eight days earlier.
- Taxon
- chrysanthemumChrysanthemumt0
- Treatment
- transgenic plantse0
- Outcome
- bloomede0
- Direction
- earliere0
- Value
- 8 dayse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transgenic plants bloomed 8 days earlier.”
t0“chrysanthemum (Chrysanthemum morifolium)”
Claim · model-written summary
GQH scions on transgenic plants flowered 12 days earlier than scions on non-transgenic plants.
- Taxon
- chrysanthemumChrysanthemumt0
- Material
- GQHe0
- Treatment
- scions grafted onto transgenic plantse0
- Outcome
- GQH and HSJQ scionse0
- Direction
- earliere0
- Value
- 12e0
- Comparator
- scions grafted onto non-transgenic plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GQH and HSJQ scions grafted onto transgenic plants were 12 and 9 days earlier than the scions grafted onto non-transgenic plants, respectively.”
t0“chrysanthemum (Chrysanthemum morifolium)”
Claim · model-written summary
HSJQ scions on transgenic plants flowered nine days earlier than scions on non-transgenic plants.
- Taxon
- chrysanthemumChrysanthemumt0
- Material
- HSJQe0
- Treatment
- scions grafted onto transgenic plantse0
- Outcome
- GQH and HSJQ scionse0
- Direction
- earliere0
- Value
- 9 dayse0
- Comparator
- scions grafted onto non-transgenic plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GQH and HSJQ scions grafted onto transgenic plants were 12 and 9 days earlier than the scions grafted onto non-transgenic plants, respectively.”
t0“chrysanthemum (Chrysanthemum morifolium)”
The same questions in other papers
loss of function · phenology · ChrysanthemumResults agree
4 papers in the same direction as this paper
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.