Abstract
Age, as a threshold of floral competence acquisition, prevents precocious flowering when there is insufficient biomass, and ensures flowering independent of environmental conditions; however, the underlying regulatory mechanisms are largely unknown. In this study, silencing the expression of a nuclear factor gene, CmNF-YB8, from the short day plant chrysanthemum (Chrysanthemum morifolium), results in precocious transition from juvenile to adult, as well as early flowering, regardless of day length conditions. The expression of SQUAMOSA PROMOTER BINDING-LIKE (SPL) family members, SPL3, SPL5, and SPL9, is upregulated in CmNF-YB8-RNAi plants, while expression of the microRNA, cmo-MIR156, is downregulated. In addition, CmNF-YB8 is shown to bind to the promoter of the cmo-MIR156 gene. Ectopic expression of cmo-miR156, using a virus-based microRNA expression system, restores the early flowering phenotype caused by CmNF-YB8 silencing. These results show that CmNF-YB8 influences flowering time through directly regulating the expression of cmo-MIR156 in the aging pathway.The mechanisms by which plant age regulates flowering remain incompletely understood. Here the authors show that age dependent regulation of SPL transcription factors by miR156 influence flowering via control of NF-YB8 expression in Chrysanthemum.
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Claims 6
Claim · model-written summary
Silencing CmNF-YB8 caused precocious juvenile to adult transition regardless of day length.
- Taxon
- chrysanthemum
.jpeg?width=60)
Chrysanthemume0 - Treatment
- silencing the expression of a nuclear factor gene, CmNF-YB8e0
- Gene
- CmNF-YB8e0
- Outcome
- transition from juvenile to adulte0
- Direction
- precociouse0
- Qualifier
- regardless of day length conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpeg?width=500)
Verbatim
e0“silencing the expression of a nuclear factor gene, CmNF-YB8, from the short day plant chrysanthemum (Chrysanthemum morifolium), results in precocious transition from juvenile to adult, as well as early flowering, regardless of day length conditions.”
Claim · model-written summary
Silencing CmNF-YB8 caused early flowering regardless of day length.
- Taxon
- chrysanthemumChrysanthemume0
- Treatment
- silencing the expression of a nuclear factor gene, CmNF-YB8e0
- Gene
- CmNF-YB8e0
- Outcome
- floweringe0
- Direction
- earlye0
- Qualifier
- regardless of day length conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing the expression of a nuclear factor gene, CmNF-YB8, from the short day plant chrysanthemum (Chrysanthemum morifolium), results in precocious transition from juvenile to adult, as well as early flowering, regardless of day length conditions.”
Claim · model-written summary
SPL3, SPL5 and SPL9 expression was upregulated in CmNF-YB8-RNAi plants.
- Taxon
- chrysanthemumChrysanthemumt0
- Material
- CmNF-YB8-RNAi plantse0
- Gene
- SPL3, SPL5, and SPL9e0
- Outcome
- expressione0
- Direction
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of SQUAMOSA PROMOTER BINDING-LIKE (SPL) family members, SPL3, SPL5, and SPL9, is upregulated in CmNF-YB8-RNAi plants, while expression of the microRNA, cmo-MIR156, is downregulated.”
t0“chrysanthemum”
Claim · model-written summary
cmo-MIR156 expression was downregulated in CmNF-YB8-RNAi plants.
- Taxon
- chrysanthemumChrysanthemumt0
- Material
- CmNF-YB8-RNAi plantse0
- Gene
- cmo-MIR156e0
- Outcome
- expressione0
- Direction
- downregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of SQUAMOSA PROMOTER BINDING-LIKE (SPL) family members, SPL3, SPL5, and SPL9, is upregulated in CmNF-YB8-RNAi plants, while expression of the microRNA, cmo-MIR156, is downregulated.”
t0“chrysanthemum”
Claim · model-written summary
CmNF-YB8 bound the cmo-MIR156 promoter.
- Taxon
- chrysanthemumChrysanthemumt0
- Gene
- CmNF-YB8e0
- Outcome
- bind to the promoter of the cmo-MIR156 genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CmNF-YB8 is shown to bind to the promoter of the cmo-MIR156 gene.”
t0“chrysanthemum”
Claim · model-written summary
Ectopic cmo-miR156 expression restored the early flowering phenotype caused by CmNF-YB8 silencing.
- Taxon
- chrysanthemumChrysanthemumt0
- Treatment
- Ectopic expression of cmo-miR156e0
- Gene
- cmo-miR156e0
- Outcome
- early flowering phenotypee0
- Direction
- restorese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of cmo-miR156, using a virus-based microRNA expression system, restores the early flowering phenotype caused by CmNF-YB8 silencing.”
t0“chrysanthemum”
The same questions in other papers
loss of function · phenology · ChrysanthemumResults agree
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- positive
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