Abstract
Abstract Nitric oxide (NO), phospholipase D (PLD) and plasma membrane (PM) H+-ATPase play crucial roles in plant response to drought stress, but the mechanisms of their function and their relationships in this process are still unclear. Here, we investigated how NO regulate PM H+-ATPase activity in maize (Zea mays L.) root tips in drought stress via inducing PLD-derived phosphatidic acid (PA) formation. Our results showed that drought stress stimulated NO production, up-regulated ZmPLD gene expression and stimulated PLD activity, coupled with PA production. With the elevation of PA content in a specific period (6 to 24 h after treatment), the PM H+-ATPase was stimulated. The NO scavenger impaired drought-induced PLD activation, PA formation and PM H+-ATPase activation at 12 h after treatment. The PLD inhibitor impaired drought-induced PA formation and PM H+-ATPase activation at 12 h after treatment. The NO scavenger, PLD inhibitor and PM H+-ATPase inhibitor aggravated the injury caused by drought stress. Exogenous NO alleviated the injury through a further elevation and a longer maintenance of ZmPLD gene expression, PLD activity, PA content and PM H+-ATPase activity in drought stress. The stimulation effect of exogenous NO on PA formation and PM H+-ATPase activation at 12 h after treatment were partly inhibited by the PLD inhibitor. The results suggested that as an early signal, NO triggers maize plant response to drought stress through increasing PM H+-ATPase activity in root tips. PLD-derived PA acts downstream of NO in this process.
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Claims 17
Claim · model-written summary
Drought stimulated nitric oxide production in maize root tips.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“drought stress stimulated NO production”
t0“Maize”
Claim · model-written summary
Drought up-regulated ZmPLD expression.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“up-regulated ZmPLD gene expression”
t0“Maize”
Claim · model-written summary
Drought stimulated PLD activity.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stimulated PLD activity”
t0“Maize”
Claim · model-written summary
Drought stimulated phosphatidic acid production.
- Outcome
- PA productione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“coupled with PA production”
t0“Maize”
Claim · model-written summary
Plasma membrane H+-ATPase was stimulated as PA rose at 6 to 24 hours.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“With the elevation of PA content in a specific period (6 to 24 h after treatment), the PM H+-ATPase was stimulated.”
t0“Maize”
Claim · model-written summary
NO scavenger impaired drought-induced PLD activation.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The NO scavenger impaired drought-induced PLD activation, PA formation and PM H+-ATPase activation at 12 h after treatment.”
t0“Maize”
Claim · model-written summary
NO scavenger impaired drought-induced PA formation.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The NO scavenger impaired drought-induced PLD activation, PA formation and PM H+-ATPase activation at 12 h after treatment.”
t0“Maize”
Claim · model-written summary
NO scavenger impaired drought-induced H+-ATPase activation.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The NO scavenger impaired drought-induced PLD activation, PA formation and PM H+-ATPase activation at 12 h after treatment.”
t0“Maize”
Claim · model-written summary
PLD inhibitor impaired drought-induced PA formation.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The PLD inhibitor impaired drought-induced PA formation and PM H+-ATPase activation at 12 h after treatment.”
t0“Maize”
Claim · model-written summary
PLD inhibitor impaired drought-induced H+-ATPase activation.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The PLD inhibitor impaired drought-induced PA formation and PM H+-ATPase activation at 12 h after treatment.”
t0“Maize”
Claim · model-written summary
NO scavenger, PLD inhibitor and H+-ATPase inhibitor aggravated drought injury.
- Treatment
- NO scavenger, PLD inhibitor and PM H+-ATPase inhibitore0
- Outcome
- injury caused by drought stresse0
- Direction
- aggravatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The NO scavenger, PLD inhibitor and PM H+-ATPase inhibitor aggravated the injury caused by drought stress.”
t0“Maize”
Claim · model-written summary
Exogenous NO alleviated drought injury.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous NO alleviated the injury”
t0“Maize”
Claim · model-written summary
Exogenous NO further elevated and prolonged ZmPLD expression and PLD activity.
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“further elevation and a longer maintenance of ZmPLD gene expression, PLD activity”
t0“Maize”
Claim · model-written summary
Exogenous NO further elevated and prolonged PA content and H+-ATPase activity.
- Outcome
- PA content and PM H+-ATPase activitye0
Not reportedTreatmentComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PA content and PM H+-ATPase activity in drought stress”
t0“Maize”
Claim · model-written summary
PLD inhibitor partly inhibited exogenous NO stimulation of PA formation and H+-ATPase activation.
- Treatment
- PLD inhibitore0
- Outcome
- PA formation and PM H+-ATPase activatione0
- Direction
- partly inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stimulation effect of exogenous NO on PA formation and PM H+-ATPase activation at 12 h after treatment were partly inhibited by the PLD inhibitor.”
t0“Maize”
Claim · model-written summary
The authors infer NO triggers the maize drought response through increased root H+-ATPase activity.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NO triggers maize plant response to drought stress through increasing PM H+-ATPase activity in root tips.”
Claim · model-written summary
PLD-derived PA acts downstream of NO in this process.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PLD-derived PA acts downstream of NO in this process.”
t0“Maize”
The same questions in other papers
nitric oxide · molecular: metabolites · Zea mays (maize)Positive and negative results
2 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.