Abstract
Understanding the response of a crop to drought is the first step in the breeding of tolerant genotypes. In this study, two sorghum (Sorghum bicolor L.) genotypes with contrasting sensitivity to drought were subjected drought stress by withholding water for 7 days at seedling stage; physiological and protein analyses were made. Reduction percentage was recorded on leaf water content, chlorophyll a and b and total chlorophyll. Shoot and root lengths reductions were observed in the drought-sensitive Cultivar (Tabat) while the drought-tolerant line (EL9) showed an increase in shoot and root lengths under drought conditions. Drought tolerant sorghum line EL9 accumulated higher proline (26% increase) when compared to the sensitive cultivar Tabat (5% increase). Mass spectrometry analysis coupled with nanoflow UPLC was used to compare daily-watered with drought stressed (7 days) seedlings. A total of 36 protein spots were detected, of which 23 were recorded for one or both accessions under drought stress conditions only. These proteins were identified using MASCOT database search in accordance with sequence similarity with previously characterized proteins from the Uniprot database. The identified proteins were assigned to different functional categories as follows: Response to stress (35%); metabolic processes (26%); photosynthetic (13%); fatty acid biosynthesis (4%) and cell wall biogenesis/degradation (4%). Seven of the identified proteins under stress condition were unique to El9, in contrast to 4 proteins were unique to Tabat. This study showed a differential protein expression pattern of two sorghum accessions under drought stress, which will be valuable for studying the molecular mechanisms underlying drought tolerance in the future. Also, these proteins could be potential candidates for development of markers to be used in markers assisted selection.
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Claims 9
Claim · model-written summary
Drought reduced leaf water content and chlorophyll measures.
- Taxon
- Sorghum bicolor
Sorghum bicolort0 - Outcome
- leaf water content, chlorophyll a and b and total chlorophylle0
- Direction
- Reductione0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Reduction percentage was recorded on leaf water content, chlorophyll a and b and total chlorophyll.”
t0“Sorghum bicolor”
Claim · model-written summary
Drought reduced shoot and root lengths in Tabat.
- Taxon
- Sorghum bicolorSorghum bicolort0
- Material
- Tabate0
- Treatment
- drought conditionse0
- Outcome
- Shoot and root lengthse0
- Direction
- reductionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shoot and root lengths reductions were observed in the drought-sensitive Cultivar (Tabat) while the drought-tolerant line (EL9) showed an increase in shoot and root lengths under drought conditions.”
t0“Sorghum bicolor”
Claim · model-written summary
Drought increased shoot and root lengths in EL9.
- Taxon
- Sorghum bicolorSorghum bicolort0
- Material
- EL9e0
- Treatment
- drought conditionse0
- Outcome
- shoot and root lengthse0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shoot and root lengths reductions were observed in the drought-sensitive Cultivar (Tabat) while the drought-tolerant line (EL9) showed an increase in shoot and root lengths under drought conditions.”
t0“Sorghum bicolor”
Claim · model-written summary
EL9 proline increased by 26% under drought.
SorghumOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Drought tolerant sorghum line EL9 accumulated higher proline (26% increase) when compared to the sensitive cultivar Tabat (5% increase).”
Claim · model-written summary
Tabat proline increased by 5% under drought.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Drought tolerant sorghum line EL9 accumulated higher proline (26% increase) when compared to the sensitive cultivar Tabat (5% increase).”
Claim · model-written summary
Of 36 detected protein spots, 23 occurred only under drought in one or both accessions.
- Taxon
- Sorghum bicolorSorghum bicolort0
- Outcome
- protein spotse0
- Value
- 23e0
- Qualifier
- under drought stress conditions onlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 36 protein spots were detected, of which 23 were recorded for one or both accessions under drought stress conditions only.”
t0“Sorghum bicolor”
Claim · model-written summary
Identified drought-responsive proteins were assigned to stress response (35%), metabolic processes (26%), photosynthetic (13%), fatty acid biosynthesis (4%) and cell wall biogenesis/degradation (4%) categories.
- Taxon
- Sorghum bicolorSorghum bicolort0
- Outcome
- functional categoriese0
- Value
- 35%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The identified proteins were assigned to different functional categories as follows: Response to stress (35%); metabolic processes (26%); photosynthetic (13%); fatty acid biosynthesis (4%) and cell wall biogenesis/degradation (4%).”
t0“Sorghum bicolor”
Claim · model-written summary
Seven stress-responsive proteins were unique to EL9.
- Taxon
- Sorghum bicolorSorghum bicolort0
- Material
- El9e0
- Outcome
- identified proteins under stress conditione0
- Value
- Sevene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Seven of the identified proteins under stress condition were unique to El9, in contrast to 4 proteins were unique to Tabat.”
t0“Sorghum bicolor”
Claim · model-written summary
Four stress-responsive proteins were unique to Tabat.
- Taxon
- Sorghum bicolorSorghum bicolort0
- Material
- Tabate0
- Outcome
- proteins were unique to Tabate0
- Value
- 4e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Seven of the identified proteins under stress condition were unique to El9, in contrast to 4 proteins were unique to Tabat.”
t0“Sorghum bicolor”
The same questions in other papers
drought · growth · Sorghum bicolor (sorghum)Positive and negative results
4 papers in the same direction as this paper · 24 in the opposite direction
- positive
- negative
- negative
25 papers more in this group
See all 29 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.