Abstract
Bacterial isolates obtained from the rhizosphere of Arabidopsis and a plantless compost potting mix were screened for anti-oomycete activity against Phytophthora capsici, Phytophthora citricola, Phytophthora palmivora and Phytophthora cinnamomi. Three out of 48 isolates exhibited more than 65% inhibition against all tested Phytophthora species and were selected for further studies. These strains, named UQ154, UQ156 and UQ202, are closely related to Bacillus amyloliquefaciens, Bacillus velezensis and Acinetobacter sp., respectively, based on 16S rDNA sequence analysis. The isolates were evaluated for their ability to fix nitrogen, solubilize phosphate, as well as for siderophore, indole acetic acid, cell wall degrading enzymes and biofilm production. Their plant growth promoting activities were evaluated by measuring their effect on the germination percentage, root and shoot length, and seedling vigour of lettuce plants. All of these traits were significantly enhanced in plants grown from seeds inoculated with the isolates compared with control plants. Moreover, bacteria-inoculated P. capsici-infected chilli plants exhibited improved productivity based on CO2 assimilation rates. Both real-time quantitative PCR and disease severity index revealed significant decreases in pathogen load in infected chilli root tissues when plants were previously inoculated with the isolates. Biocontrol activity may result from the secretion of diketopiperazines as identified by Gas Chromatography-Mass Spectrometry analysis of bacterial cultures’ extracts. Collectively, this work demonstrates the potential of bacterial isolates to control Phytophthora infection and promote plant growth. They can, therefore be considered as candidate microbial biofertilizers and biopesticides.
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Claims 7
Claim · model-written summary
Three bacterial isolates inhibited all tested Phytophthora species by more than 65%.
- Taxon
- Arabidopsis
Arabidopsise1 - Material
- Three out of 48 isolatese0
- Interactor
- Phytophthora species
Phytophthorae0pathogen - Outcome
- inhibition against all tested Phytophthora speciese0
- Value
- more than 65%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Schema note from the model: In vitro inhibition measured without a plant host; taxon names the plant source of isolates.
Verbatim
e0“Three out of 48 isolates exhibited more than 65% inhibition against all tested Phytophthora species”
e1“rhizosphere of Arabidopsis”
Claim · model-written summary
The three selected isolates were closely related to Bacillus amyloliquefaciens, Bacillus velezensis, and Acinetobacter sp., respectively.
- Taxon
- ArabidopsisArabidopsise1
- Outcome
- closely related to Bacillus amyloliquefaciens, Bacillus velezensis and Acinetobacter sp.e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Schema note from the model: Isolate identity is not a plant outcome.
Verbatim
e0“These strains, named UQ154, UQ156 and UQ202, are closely related to Bacillus amyloliquefaciens, Bacillus velezensis and Acinetobacter sp., respectively, based on 16S rDNA sequence analysis”
e1“rhizosphere of Arabidopsis”
Claim · model-written summary
Bacterial inoculation significantly enhanced lettuce germination percentage, root and shoot length, and seedling vigour compared with controls.
- Taxon
- lettuce plants
Lactuca sativae0 - Treatment
- seeds inoculated with the isolatese0
- Interactor
- the isolatese0symbiont
- Outcome
- germination percentage, root and shoot length, and seedling vigoure0
- Direction
- enhancede0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“germination percentage, root and shoot length, and seedling vigour of lettuce plants. All of these traits were significantly enhanced in plants grown from seeds inoculated with the isolates compared with control plants”
Claim · model-written summary
Bacteria-inoculated, P. capsici-infected chilli plants had improved productivity measured by CO2 assimilation.
- Taxon
- chilli plantse0
- Treatment
- bacteria-inoculatede0
- Interactor
- P. capsici
.jpg?width=60)
Phytophthora capsicie0pathogen - Outcome
- CO2 assimilation ratese0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“bacteria-inoculated P. capsici-infected chilli plants exhibited improved productivity based on CO2 assimilation rates”
Claim · model-written summary
Prior inoculation significantly decreased pathogen load in infected chilli roots.
- Taxon
- chillie0
- Organ / stage
- root tissuese0
- Treatment
- previously inoculated with the isolatese0
- Interactor
- P. capsiciPhytophthora capsicie1pathogen
- Second interactor
- the isolatese0biocontrol_agent
- Outcome
- pathogen load in infected chilli root tissuese0
- Direction
- decreasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“significant decreases in pathogen load in infected chilli root tissues when plants were previously inoculated with the isolates”
e1“P. capsici-infected chilli plants”
Claim · model-written summary
Biocontrol activity may result from diketopiperazine secretion.
- Taxon
- chilli plantse1
- Interactor
- P. capsiciPhytophthora capsicie1pathogen
- Compound
- diketopiperazinese0
- Outcome
- Biocontrol activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Biocontrol activity may result from the secretion of diketopiperazines as identified by Gas Chromatography-Mass Spectrometry analysis of bacterial cultures’ extracts”
e1“P. capsici-infected chilli plants”
Claim · model-written summary
The bacterial isolates are candidates for microbial biofertilizers and biopesticides.
- Taxon
- lettuce plantsLactuca sativae1
- Outcome
- candidate microbial biofertilizers and biopesticidese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“They can, therefore be considered as candidate microbial biofertilizers and biopesticides”
e1“lettuce plants”
e2“chilli plants”
The same questions in other papers
inoculation · Phytophthora capsici · Capsicum (peppers)Positive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
- no effect
- negative
- negative
2 papers more in this group
See all 6 papers →inoculation · growth · Lactuca sativa (garden lettuce)Positive and negative results
96 papers in the same direction as this paper · 12 in the opposite direction · 2 reporting no difference from an alternative
- no effect
- negative
- negative
107 papers more in this group
See all 111 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.