Abstract
Phosphorus (P) deficiency is a major limitation for legume crop production. Although overall adaptations of plant roots to P deficiency have been extensively studied, only fragmentary information is available in regard to root nodule responses to P deficiency. In this study, genome wide transcriptome analysis was conducted using RNA-seq analysis in soybean nodules grown under P-sufficient (500 μM KH2PO4) and P-deficient (25 μM KH2PO4) conditions to investigate molecular mechanisms underlying soybean (Glycine max) nodule adaptation to phosphate (Pi) starvation. Phosphorus deficiency significantly decreased soybean nodule growth and nitrogenase activity. Nodule Pi concentrations declined by 49% in response to P deficiency, but this was well below the 87% and 88% decreases observed in shoots and roots, respectively. Nodule transcript profiling revealed that a total of 2055 genes exhibited differential expression patterns between Pi sufficient and deficient conditions. A set of (differentially expressed genes) DEGs appeared to be involved in maintaining Pi homeostasis in soybean nodules, including eight Pi transporters (PTs), eight genes coding proteins containing the SYG1/PHO81/XPR1 domain (SPXs), and 16 purple acid phosphatases (PAPs). The results suggest that a complex transcriptional regulatory network participates in soybean nodule adaption to Pi starvation, most notable a Pi signaling pathway, are involved in maintaining Pi homeostasis in nodules.
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Claims 10
Claim · model-written summary
Phosphorus deficiency significantly decreased soybean nodule growth and nitrogenase activity.
- Taxon
- soybean
Glycine maxe0 - Organ / stage
- nodulee1
- Treatment
- Phosphorus deficiencye1
- Outcome
- nodule growth and nitrogenase activitye1
- Direction
- decreasede1
- Comparator
- P-sufficient (500 μM KH2PO4)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in soybean nodules grown under P-sufficient (500 μM KH2PO4) and P-deficient (25 μM KH2PO4) conditions”
e1“Phosphorus deficiency significantly decreased soybean nodule growth and nitrogenase activity”
Claim · model-written summary
Under P deficiency, nodule Pi concentration declined by 49%, well below the declines in shoots and roots.
- Taxon
- soybeanGlycine maxe0
- Organ / stage
- Nodulee2
- Treatment
- P deficiencye2
- Outcome
- Pi concentrationse2
- Direction
- declinede2
- Value
- 49%e2
- Qualifier
- well below the 87% and 88% decreases observed in shoots and rootse2
- Comparator
- P-sufficient (500 μM KH2PO4)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in soybean nodules grown under P-sufficient (500 μM KH2PO4) and P-deficient (25 μM KH2PO4) conditions”
e2“Nodule Pi concentrations declined by 49% in response to P deficiency, but this was well below the 87% and 88% decreases observed in shoots and roots, respectively”
Claim · model-written summary
Under P deficiency, Pi concentration in soybean shoots decreased by 87%.
- Taxon
- soybeanGlycine maxe0
- Organ / stage
- shootse2
- Treatment
- P deficiencye2
- Outcome
- Pi concentrationse2
- Direction
- decreasese2
- Value
- 87%e2
- Comparator
- P-sufficient (500 μM KH2PO4)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in soybean nodules grown under P-sufficient (500 μM KH2PO4) and P-deficient (25 μM KH2PO4) conditions”
e2“Nodule Pi concentrations declined by 49% in response to P deficiency, but this was well below the 87% and 88% decreases observed in shoots and roots, respectively”
Claim · model-written summary
Under P deficiency, Pi concentration in soybean roots decreased by 88%.
- Taxon
- soybeanGlycine maxe0
- Organ / stage
- rootse2
- Treatment
- P deficiencye2
- Outcome
- Pi concentrationse2
- Direction
- decreasese2
- Value
- 88%e2
- Comparator
- P-sufficient (500 μM KH2PO4)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in soybean nodules grown under P-sufficient (500 μM KH2PO4) and P-deficient (25 μM KH2PO4) conditions”
e2“Nodule Pi concentrations declined by 49% in response to P deficiency, but this was well below the 87% and 88% decreases observed in shoots and roots, respectively”
Claim · model-written summary
Pi deficiency changed the expression of 2055 genes in soybean nodules relative to Pi sufficiency.
- Taxon
- soybeanGlycine maxe0
- Treatment
- deficient conditionse3
- Outcome
- differential expression patternse3
- Direction
- differential expressione3
- Value
- 2055 genese3
- Comparator
- Pi sufficiente3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in soybean nodules grown under P-sufficient (500 μM KH2PO4) and P-deficient (25 μM KH2PO4) conditions”
e3“a total of 2055 genes exhibited differential expression patterns between Pi sufficient and deficient conditions”
Claim · model-written summary
Eight Pi transporter genes were among the differentially expressed genes of soybean nodules involved in Pi homeostasis.
- Taxon
- soybeanGlycine maxe0
- Gene
- Pi transporters (PTs)e4
- Outcome
- DEGse4
- Value
- eighte4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in soybean nodules grown under P-sufficient (500 μM KH2PO4) and P-deficient (25 μM KH2PO4) conditions”
e4“A set of (differentially expressed genes) DEGs appeared to be involved in maintaining Pi homeostasis in soybean nodules, including eight Pi transporters (PTs)”
Claim · model-written summary
Eight SPX-domain genes were among the differentially expressed genes of soybean nodules.
- Taxon
- soybeanGlycine maxe0
- Outcome
- genes coding proteins containing the SYG1/PHO81/XPR1 domaine5
- Value
- eighte5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in soybean nodules grown under P-sufficient (500 μM KH2PO4) and P-deficient (25 μM KH2PO4) conditions”
e5“eight genes coding proteins containing the SYG1/PHO81/XPR1 domain (SPXs)”
Claim · model-written summary
Sixteen purple acid phosphatase genes were among the differentially expressed genes of soybean nodules.
- Taxon
- soybeanGlycine maxe0
- Outcome
- purple acid phosphatases (PAPs)e6
- Value
- 16e6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in soybean nodules grown under P-sufficient (500 μM KH2PO4) and P-deficient (25 μM KH2PO4) conditions”
e6“and 16 purple acid phosphatases (PAPs)”
Claim · model-written summary
A complex transcriptional regulatory network participates in soybean nodule adaptation to Pi starvation.
- Taxon
- soybeanGlycine maxe7
- Outcome
- adaption to Pi starvatione7
- Qualifier
- complex transcriptional regulatory networke7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“The results suggest that a complex transcriptional regulatory network participates in soybean nodule adaption to Pi starvation”
Claim · model-written summary
A Pi signaling pathway is involved in maintaining Pi homeostasis in soybean nodules.
- Taxon
- soybeanGlycine maxe0
- Outcome
- maintaining Pi homeostasis in nodulese1
- Qualifier
- a Pi signaling pathwaye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in soybean nodules grown under P-sufficient (500 μM KH2PO4) and P-deficient (25 μM KH2PO4) conditions”
e1“a Pi signaling pathway, are involved in maintaining Pi homeostasis in nodules”
The same questions in other papers
phosphorus · physiology: enzyme activity · Glycine max (soybean)Positive and negative results
6 papers in the same direction as this paper · 3 in the opposite direction
This paper is about lacking the nutrient, so it counts the other way round here.
- negative
- negative
- positive
6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.