Abstract
When working with pollen, it is important to keep its functional parameters for some time. To do this, you need to choose the best conditions to preserve pollen germination. The influence of different method of pollen storage of inbred plants of beetroot on its functional characteristics, at germination on artificial nutrient medium under conditions in vitro. When storing pollen in eppendorf tube for the second-third day, the pollen germination decreased by 3-10 times compared to the initial value, after a week of storage, pollen was in conglomerates, a lot of burst pollen. When stored on the sprigs for the seventh to eighth days, the decrease in pollen germination was no more than 30% of the control, and the growth rate of the pollen tube in most samples remained approximately at the control level. For a comparative analysis of the germination of pollen of inbred plants beetroot when evaluating a large set of samples, it is recommended to store flowering twigs in paper bags at a reduced temperature (10-12°C). This is important when working with a large set of samples where it is not possible to compare them at the same time.
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Claims 5
Claim · model-written summary
Pollen germination after two to three days in an eppendorf tube fell 3–10 fold from its initial value.
- Taxon
- TABLE BEET
Beta vulgaris subsp. vulgarist0 - Organ / stage
- pollene0
- Treatment
- storing pollen in eppendorf tubee0
- Outcome
- pollen germinatione0
- Direction
- decreasede0
- Value
- 3-10 timese0
- Comparator
- initial valuee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When storing pollen in eppendorf tube for the second-third day, the pollen germination decreased by 3-10 times compared to the initial value”
t0“TABLE BEET”
Claim · model-written summary
After a week in an eppendorf tube, pollen formed conglomerates and many grains burst.
- Taxon
- TABLE BEETBeta vulgaris subsp. vulgarist0
- Organ / stage
- pollene0
- Outcome
- pollen was in conglomerates, a lot of burst pollene0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“after a week of storage, pollen was in conglomerates, a lot of burst pollen”
t0“TABLE BEET”
Claim · model-written summary
On sprigs at seven to eight days, pollen germination decreased by no more than 30% of control.
- Taxon
- TABLE BEETBeta vulgaris subsp. vulgarist0
- Organ / stage
- pollene0
- Treatment
- stored on the sprigse0
- Outcome
- pollen germinatione0
- Direction
- decreasee0
- Value
- 30%e0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When stored on the sprigs for the seventh to eighth days, the decrease in pollen germination was no more than 30% of the control”
t0“TABLE BEET”
Claim · model-written summary
On sprigs, pollen tube growth rate stayed approximately at control level in most samples.
- Taxon
- TABLE BEETBeta vulgaris subsp. vulgarist0
- Treatment
- stored on the sprigse0
- Outcome
- growth rate of the pollen tubee0
- Direction
- remained approximatelye0
- Qualifier
- in most samplese0
- Comparator
- control levele0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When stored on the sprigs for the seventh to eighth days, the decrease in pollen germination was no more than 30% of the control, and the growth rate of the pollen tube in most samples remained approximately at the control level”
t0“TABLE BEET”
Claim · model-written summary
The authors recommend flowering twigs in paper bags at 10–12°C for comparing many beetroot pollen samples.
- Taxon
- TABLE BEETBeta vulgaris subsp. vulgarist0
- Organ / stage
- flowering twigse0
- Outcome
- store flowering twigs in paper bags at a reduced temperature (10-12°C)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it is recommended to store flowering twigs in paper bags at a reduced temperature (10-12°C)”
t0“TABLE BEET”
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See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.