Abstract
The development of soybeans tolerant to the soybean aphid [Aphis glycines Matsumura (Hemiptera: Aphididae)] remains unexplored. The objectives of this research were to determine the susceptibility of two high-yielding soybean [Glycine max (L.) Merrill (Fabales: Fabaceae)] genotypes involved in a breeding platform to develop aphid-tolerant recombinant inbred lines (RILs); characterize the peroxidase activity and relative expression of peroxidase transcripts in the parents of RILs; and identify an assay to phenotype aphid-tolerant RILs. Enzyme kinetic assays documented the total peroxidase activity for tolerant (KS4202), susceptible (SD76R), and two high-yielding (U09-105007 and U11-611112) soybeans during two vegetative stages (V1 and V3) at three sampling days (D4, D6, and D8 after aphid introduction). Enzyme kinetic assays showed that V3 infested tolerant and U11-611112 plants had significantly higher peroxidase activity than their respective control plants at D4, and infested tolerant plants were also higher than control plants at D6. There were no apparent trends when comparing the expression of peroxidase-specific transcripts in the absence of aphids (basal levels) in both V1 and V3. Relative expression analyses of two peroxidase transcripts (PRX52 and PRX2) performed to compare differences among the soybean genotypes indicated that, despite basal levels being similar for the treatments analyzed, tolerant soybeans had a tendency for a higher expression of PRX52 in the presence of aphids. Based on the different patterns observed and the feasibility of analyses performed in this study, enzyme kinetics using V3 infested plants may be a marker for screening RILs in a breeding program targeting the development of aphid-tolerant soybeans.
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Claims 6
Claim · model-written summary
At V3 on day 4, aphid-infested tolerant soybean plants had significantly higher peroxidase activity than controls.
- Taxon
- Soybean
Glycine maxt0 - Material
- tolerante0
- Organ / stage
- V3e0
- Treatment
- infestede0
- Interactor
- Soybean Aphid
.jpg?width=60)
Aphis glycinest1pest - Outcome
- peroxidase activitye0
- Direction
- highere0
- Qualifier
- D4e0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“V3 infested tolerant and U11-611112 plants had significantly higher peroxidase activity than their respective control plants at D4”
t0“Phenotyping Soybean Populations”
t1“Soybean Aphid”
Claim · model-written summary
At V3 on day 4, aphid-infested U11-611112 soybean plants had significantly higher peroxidase activity than controls.
- Taxon
- SoybeanGlycine maxt0
- Material
- U11-611112e0
- Organ / stage
- V3e0
- Treatment
- infestede0
- Interactor
- Soybean AphidAphis glycinest1pest
- Outcome
- peroxidase activitye0
- Direction
- highere0
- Qualifier
- D4e0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“V3 infested tolerant and U11-611112 plants had significantly higher peroxidase activity than their respective control plants at D4”
t0“Phenotyping Soybean Populations”
t1“Soybean Aphid”
Claim · model-written summary
At day 6, aphid-infested tolerant plants also had higher peroxidase activity than controls.
- Taxon
- SoybeanGlycine maxt0
- Material
- tolerante0
- Treatment
- infestede0
- Interactor
- Soybean AphidAphis glycinest1pest
- Outcome
- peroxidase activitye1
- Direction
- highere0
- Qualifier
- D6e0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“infested tolerant plants were also higher than control plants at D6”
e1“peroxidase activity than their respective control plants at D4”
t0“Phenotyping Soybean Populations”
t1“Soybean Aphid”
Claim · model-written summary
Basal expression of peroxidase-specific transcripts showed no apparent trend across V1 and V3.
- Taxon
- SoybeanGlycine maxt0
- Organ / stage
- V1 and V3e0
- Interactor
- Soybean AphidAphis glycinest1pest
- Outcome
- expression of peroxidase-specific transcriptse0
- Direction
- no apparent trendse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There were no apparent trends when comparing the expression of peroxidase-specific transcripts in the absence of aphids (basal levels) in both V1 and V3”
t0“Phenotyping Soybean Populations”
t1“Soybean Aphid”
Claim · model-written summary
Aphid-exposed tolerant soybeans tended toward higher PRX52 expression.
- Taxon
- soybeansGlycine maxe0
- Material
- tolerante0
- Interactor
- aphidsAphidomorphae0pest
- Gene
- PRX52e0
- Outcome
- expression of PRX52e0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tolerant soybeans had a tendency for a higher expression of PRX52 in the presence of aphids”
Claim · model-written summary
The authors propose enzyme kinetics on V3 infested plants as a marker for screening aphid-tolerant RILs.
- Taxon
- soybeansGlycine maxe0
- Organ / stage
- V3e0
- Interactor
- aphide0pest
- Outcome
- enzyme kinetics using V3 infested plants may be a marker for screening RILse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enzyme kinetics using V3 infested plants may be a marker for screening RILs in a breeding program targeting the development of aphid-tolerant soybeans”
The same questions in other papers
infection · Aphis glycines · Glycine max (soybean)Positive and negative results
3 papers in the same direction as this paper · 5 in the opposite direction
- negative
- no effect
- negative
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.