Abstract
The relationship between polygalacturonase activity and abscission of citrus leaf explants was studied. Determination of polygalacturonase activity in citrus tissues requires concentration of the enzyme, use of a proper assay method, and inhibition of an oxidase present in the extracts which oxidizes the reaction products of the polygalacturonase. The polygalacturonase from citrus leaf explants is an exopolygalacturonase and appears to be a soluble enzyme. Polygalacturonase activity increased during abscission of citrus leaf explants and was localized in the separation layer. Ethylene accelerated the increase in polygalacturonase activity, but its effect was evident only after at least an 8-hour lag period. 2,4-Dichlorophenoxyacetic acid and cycloheximide inhibited abscission and polygalacturonase activity. It is concluded that polygalacturonase, in addition to cellulase, plays a role in abscission.
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Claims 8
Claim · model-written summary
Determination of polygalacturonase activity in citrus tissues requires enzyme concentration, a proper assay method and inhibition of an oxidase present in the extracts.
CitrusNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Determination of polygalacturonase activity in citrus tissues requires concentration of the enzyme, use of a proper assay method, and inhibition of an oxidase present in the extracts”
Claim · model-written summary
Polygalacturonase from citrus leaf explants is an exopolygalacturonase and appears to be a soluble enzyme.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The polygalacturonase from citrus leaf explants is an exopolygalacturonase and appears to be a soluble enzyme”
Claim · model-written summary
Polygalacturonase activity increased during abscission of citrus leaf explants.
- Organ / stage
- leaf explantse0
- Gene
- Polygalacturonasee0
- Outcome
- Polygalacturonase activitye0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Polygalacturonase activity increased during abscission of citrus leaf explants”
Claim · model-written summary
Polygalacturonase activity was localized in the separation layer of citrus leaf explants.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Polygalacturonase activity increased during abscission of citrus leaf explants and was localized in the separation layer”
Claim · model-written summary
Ethylene accelerated the increase in polygalacturonase activity, but only after at least an 8-hour lag period.
- Treatment
- Ethylenee1
- Outcome
- polygalacturonase activitye1
- Direction
- acceleratede1
- Qualifier
- only after at least an 8-hour lag periode1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The relationship between polygalacturonase activity and abscission of citrus leaf explants”
e1“Ethylene accelerated the increase in polygalacturonase activity, but its effect was evident only after at least an 8-hour lag period”
Claim · model-written summary
2,4-Dichlorophenoxyacetic acid inhibited abscission and polygalacturonase activity in citrus leaf explants.
- Treatment
- 2,4-Dichlorophenoxyacetic acide1
- Outcome
- abscission and polygalacturonase activitye1
- Direction
- inhibitede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The relationship between polygalacturonase activity and abscission of citrus leaf explants”
e1“2,4-Dichlorophenoxyacetic acid and cycloheximide inhibited abscission and polygalacturonase activity”
Claim · model-written summary
Cycloheximide inhibited abscission and polygalacturonase activity in citrus leaf explants.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The relationship between polygalacturonase activity and abscission of citrus leaf explants”
e1“2,4-Dichlorophenoxyacetic acid and cycloheximide inhibited abscission and polygalacturonase activity”
Claim · model-written summary
The authors conclude that polygalacturonase, in addition to cellulase, plays a role in abscission.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The relationship between polygalacturonase activity and abscission of citrus leaf explants”
e1“polygalacturonase, in addition to cellulase, plays a role in abscission”
The same questions in other papers
ethene · physiology: enzyme activity · CitrusPositive and negative results
1 paper in the same direction as this paper · 3 in the opposite direction
- no effect
- no effect
- no effect
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.