Abstract
Abstract Glycolate oxidase (GOX)-dependent production of H 2 O 2 in response to pathogens and its function in disease resistance are still poorly understood. In this study, we performed genome-wide identification of GOX gene family in Nicotiana benthamiana and analyzed their function in various types of disease resistance. Sixteen GOX genes were identified in N . benthamiana genome. They consisted of GOX and HAOX groups. All but two NbGOX proteins contained an alpha_hydroxyacid_oxid_FMN domain with extra 43–52 amino acids compared to that of FMN-dependent alpha-hydroxyacid oxidizing enzymes (NCBI-CDD cd02809). Silencing of three NbGOX family genes NbHAOX8 , NbGOX1 and NbGOX4 differently affected resistance to various pathogens including Tobacco rattle virus , Xanthomonas oryzae pv. oryzae ( Xoo ) and Sclerotinia sclerotiorum . Effect of these genes on resistance to Xoo is well correlated with that on Xoo –responsive H 2 O 2 accumulation. Additionally, silencing of these genes enhanced PAMP-triggered immunity as shown by increased flg22-elicited H 2 O 2 accumulation in NbGOX -silenced plants. These NbGOX family genes were distinguishable in altering expression of defense genes. Analysis of mutual effect on gene expression indicated that NbGOX4 might function through repressing NbHAOX8 and NbGOX1 . Collectively, our results reveal the important roles and functional complexity of GOX genes in disease resistance in N . benthamiana .
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Claims 10
Claim · model-written summary
Sixteen GOX genes were identified in N. benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianat0 - Gene
- GOX genese0
- Outcome
- GOX genese0
- Value
- Sixteene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sixteen GOX genes were identified in N . benthamiana genome.”
t0“Nicotiana benthamiana”
Claim · model-written summary
The identified genes consisted of GOX and HAOX groups.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- GOX and HAOXe0
- Outcome
- GOX and HAOX groupse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“They consisted of GOX and HAOX groups.”
t0“Nicotiana benthamiana”
Claim · model-written summary
All but two NbGOX proteins had an alpha_hydroxyacid_oxid_FMN domain with 43–52 extra amino acids.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- NbGOX proteinse0
- Outcome
- alpha_hydroxyacid_oxid_FMN domaine0
- Value
- 43–52 amino acidse0
- Comparator
- FMN-dependent alpha-hydroxyacid oxidizing enzymese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All but two NbGOX proteins contained an alpha_hydroxyacid_oxid_FMN domain with extra 43–52 amino acids compared to that of FMN-dependent alpha-hydroxyacid oxidizing enzymes”
t0“Nicotiana benthamiana”
Claim · model-written summary
Silencing NbHAOX8, NbGOX1 and NbGOX4 had different effects on resistance to Tobacco rattle virus.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Silencing of three NbGOX family genese0
- Interactor
- Tobacco rattle virusTobacco rattle viruse0pathogen
- Gene
- NbHAOX8 , NbGOX1 and NbGOX4e0
- Outcome
- resistance to various pathogense0
- Direction
- differently affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of three NbGOX family genes NbHAOX8 , NbGOX1 and NbGOX4 differently affected resistance to various pathogens including Tobacco rattle virus , Xanthomonas oryzae pv. oryzae ( Xoo ) and Sclerotinia sclerotiorum .”
t0“Nicotiana benthamiana”
Claim · model-written summary
Silencing the three genes had different effects on resistance to Xanthomonas oryzae pv. oryzae.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Silencing of three NbGOX family genese0
- Interactor
- Xanthomonas oryzae pv. oryzae
Xanthomonas oryzae pv. oryzaee0pathogen - Gene
- NbHAOX8 , NbGOX1 and NbGOX4e0
- Outcome
- resistance to various pathogense0
- Direction
- differently affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of three NbGOX family genes NbHAOX8 , NbGOX1 and NbGOX4 differently affected resistance to various pathogens including Tobacco rattle virus , Xanthomonas oryzae pv. oryzae ( Xoo ) and Sclerotinia sclerotiorum .”
t0“Nicotiana benthamiana”
Claim · model-written summary
Silencing the three genes had different effects on resistance to Sclerotinia sclerotiorum.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Silencing of three NbGOX family genese0
- Interactor
- Sclerotinia sclerotiorum
Sclerotinia sclerotiorume0pathogen - Gene
- NbHAOX8 , NbGOX1 and NbGOX4e0
- Outcome
- resistance to various pathogense0
- Direction
- differently affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of three NbGOX family genes NbHAOX8 , NbGOX1 and NbGOX4 differently affected resistance to various pathogens including Tobacco rattle virus , Xanthomonas oryzae pv. oryzae ( Xoo ) and Sclerotinia sclerotiorum .”
t0“Nicotiana benthamiana”
Claim · model-written summary
Effects on Xoo resistance correlated with effects on Xoo-responsive hydrogen peroxide accumulation.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Interactor
- Xooe0pathogen
- Gene
- these genese0
- Outcome
- resistance to Xooe0
- Qualifier
- Xoo –responsive H 2 O 2 accumulatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Effect of these genes on resistance to Xoo is well correlated with that on Xoo –responsive H 2 O 2 accumulation.”
t0“Nicotiana benthamiana”
Claim · model-written summary
Silencing NbGOX genes increased flg22-elicited hydrogen peroxide accumulation.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- silencing of these genese0
- Gene
- NbGOXe0
- Outcome
- flg22-elicited H 2 O 2 accumulatione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of these genes enhanced PAMP-triggered immunity as shown by increased flg22-elicited H 2 O 2 accumulation in NbGOX -silenced plants.”
t0“Nicotiana benthamiana”
Claim · model-written summary
NbGOX family genes differed in their effects on defense-gene expression.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- NbGOX family genese0
- Outcome
- expression of defense genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These NbGOX family genes were distinguishable in altering expression of defense genes.”
t0“Nicotiana benthamiana”
Claim · model-written summary
NbGOX4 may act by repressing NbHAOX8 and NbGOX1.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- NbGOX4e0
- Outcome
- repressing NbHAOX8 and NbGOX1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NbGOX4 might function through repressing NbHAOX8 and NbGOX1 .”
t0“Nicotiana benthamiana”
The same questions in other papers
loss of function · physiology: oxidative · Nicotiana benthamianaPositive and negative results
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