Abstract
Changes in catalase activity during the development of the Rhizobium-legume symbiosis as well as its response in salinized plants of Phaseolus vulgaris and Medicago sativa, was studied. Besides, it was examined the behavior of the enzyme, isolated from leaves and root nodules, during in vitro incubation with NaCl doses. Nodule catalase activities of both legumes were assayed with several enzyme inhibitors and also purified. Leaf catalase activity of Phaseolus vulgaris and Medicago sativa decreased and increased respectively throughout the ontogeny, but root nodule catalase kept a high and stable value. This last result suggests that both legumes require the maintenance of high nodule catalase in nitrogen-fixing nodules. Under salt stress conditions leaf and nodule catalase activity decreased in both, grain and pasture legumes. Because catalase from leaf of Medicago sativa and nodules of Phaseolus vulgaris were relatively sensitive to NaCl during in vitro experiments, the detoxifying role of this enzyme for H2O2 should be limited in such conditions. Both catalases, from determinate and indeterminate nodules, were affected neither by oxygen nor superoxide radicals but showed a strong (Phaseolus vulgaris) or partial (Medicago sativa) inhibition with dithiothreitol, dithionite and b-mercaptoethanol. Besides, cyanide was the most potent inhibitor of nodule catalases. Finally, catalases partially purified by immobilized metal ion affinity chromatography migrated at 42 (Phaseolus vulgaris) and 46 kDa (Medicago sativa) on SDS-PAGE, whereas native forms on sephacryl S-300 columns exhibited a molecular mass of 59 and 48 kDa (Phaseolus vulgaris) and 88 and 53 kDa (Medicago sativa). 2007 Elsevier Masson SAS. All rights reserved.
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Claims 16
Claim · model-written summary
Leaf catalase activity decreased over development in Phaseolus vulgaris.
- Taxon
- Phaseolus vulgaris
Phaseolus vulgarise0 - Outcome
- Leaf catalase activitye0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Leaf catalase activity of Phaseolus vulgaris and Medicago sativa decreased and increased respectively throughout the ontogeny”
Claim · model-written summary
Leaf catalase activity increased over development in Medicago sativa.
- Taxon
- Medicago sativa
Medicago sativae0 - Outcome
- Leaf catalase activitye0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Leaf catalase activity of Phaseolus vulgaris and Medicago sativa decreased and increased respectively throughout the ontogeny”
Claim · model-written summary
Root nodule catalase remained high and stable in both legumes.
- Taxon
- Phaseolus vulgaris and Medicago sativaMedicago sativaPhaseolus vulgarise1
- Organ / stage
- root nodulee0
- Outcome
- root nodule catalasee0
- Direction
- stablee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“root nodule catalase kept a high and stable value”
e1“plants of Phaseolus vulgaris and Medicago sativa”
Claim · model-written summary
The authors infer that both legumes require high nodule catalase in nitrogen fixing nodules.
- Taxon
- Phaseolus vulgaris and Medicago sativaMedicago sativaPhaseolus vulgarise1
- Organ / stage
- nitrogen-fixing nodulese0
- Outcome
- maintenance of high nodule catalasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both legumes require the maintenance of high nodule catalase in nitrogen-fixing nodules”
e1“plants of Phaseolus vulgaris and Medicago sativa”
Claim · model-written summary
Salt stress decreased leaf and nodule catalase activity in both legumes.
- Taxon
- Phaseolus vulgaris and Medicago sativaMedicago sativaPhaseolus vulgarise1
- Treatment
- salt stresse0
- Outcome
- leaf and nodule catalase activitye0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under salt stress conditions leaf and nodule catalase activity decreased in both, grain and pasture legumes.”
e1“plants of Phaseolus vulgaris and Medicago sativa”
Claim · model-written summary
Medicago sativa leaf catalase was relatively sensitive to NaCl in vitro.
- Taxon
- Medicago sativaMedicago sativae0
- Treatment
- NaCle0
- Outcome
- catalase from leafe0
- Direction
- relatively sensitivee0
- Setting
- in vitro experimentse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“catalase from leaf of Medicago sativa and nodules of Phaseolus vulgaris were relatively sensitive to NaCl during in vitro experiments”
Claim · model-written summary
Phaseolus vulgaris nodule catalase was relatively sensitive to NaCl in vitro.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Treatment
- NaCle0
- Outcome
- catalase from leaf of Medicago sativa and nodules of Phaseolus vulgarise0
- Direction
- relatively sensitivee0
- Setting
- in vitro experimentse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“catalase from leaf of Medicago sativa and nodules of Phaseolus vulgaris were relatively sensitive to NaCl during in vitro experiments”
Claim · model-written summary
The authors suggest that catalase detoxification of H2O2 is limited under NaCl exposure.
- Taxon
- Medicago sativa and nodules of Phaseolus vulgarisMedicago sativaPhaseolus vulgarise1
- Outcome
- detoxifying role of this enzyme for H2O2e0
- Qualifier
- in such conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the detoxifying role of this enzyme for H2O2 should be limited in such conditions”
e1“catalase from leaf of Medicago sativa and nodules of Phaseolus vulgaris were relatively sensitive to NaCl during in vitro experiments”
Claim · model-written summary
Nodule catalases were unaffected by oxygen or superoxide radicals.
- Taxon
- Phaseolus vulgaris and Medicago sativaMedicago sativaPhaseolus vulgarise1
- Treatment
- oxygen nor superoxide radicalse0
- Outcome
- Both catalasese0
- Direction
- affected neithere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both catalases, from determinate and indeterminate nodules, were affected neither by oxygen nor superoxide radicals”
e1“plants of Phaseolus vulgaris and Medicago sativa”
Claim · model-written summary
Reducing agents strongly inhibited Phaseolus vulgaris nodule catalase.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Treatment
- dithiothreitol, dithionite and b-mercaptoethanole0
- Outcome
- inhibitione0
- Direction
- stronge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“strong (Phaseolus vulgaris) or partial (Medicago sativa) inhibition with dithiothreitol, dithionite and b-mercaptoethanol”
Claim · model-written summary
Reducing agents partially inhibited Medicago sativa nodule catalase.
- Taxon
- Medicago sativaMedicago sativae0
- Treatment
- dithiothreitol, dithionite and b-mercaptoethanole0
- Outcome
- inhibitione0
- Direction
- partiale0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“strong (Phaseolus vulgaris) or partial (Medicago sativa) inhibition with dithiothreitol, dithionite and b-mercaptoethanol”
Claim · model-written summary
Cyanide was the most potent inhibitor of nodule catalases.
- Taxon
- Phaseolus vulgaris and Medicago sativaMedicago sativaPhaseolus vulgarise1
- Treatment
- cyanidee0
- Outcome
- inhibitor of nodule catalasese0
- Direction
- most potente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cyanide was the most potent inhibitor of nodule catalases”
e1“plants of Phaseolus vulgaris and Medicago sativa”
Claim · model-written summary
Purified Phaseolus vulgaris catalase migrated at 42 kDa on SDS-PAGE.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Outcome
- migratede0
- Value
- 42e0
- Setting
- SDS-PAGEe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“migrated at 42 (Phaseolus vulgaris) and 46 kDa (Medicago sativa) on SDS-PAGE”
Claim · model-written summary
Purified Medicago sativa catalase migrated at 46 kDa on SDS-PAGE.
- Taxon
- Medicago sativaMedicago sativae0
- Outcome
- migratede0
- Value
- 46 kDae0
- Setting
- SDS-PAGEe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“migrated at 42 (Phaseolus vulgaris) and 46 kDa (Medicago sativa) on SDS-PAGE”
Claim · model-written summary
Native Phaseolus vulgaris catalase forms had molecular masses of 59 and 48 kDa.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Outcome
- molecular masse0
- Value
- 59 and 48 kDae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“native forms on sephacryl S-300 columns exhibited a molecular mass of 59 and 48 kDa (Phaseolus vulgaris)”
Claim · model-written summary
Native Medicago sativa catalase forms had molecular masses of 88 and 53 kDa.
- Taxon
- Medicago sativaMedicago sativae0
- Outcome
- molecular masse0
- Value
- 88 and 53 kDae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“native forms on sephacryl S-300 columns exhibited a molecular mass of 59 and 48 kDa (Phaseolus vulgaris) and 88 and 53 kDa (Medicago sativa)”
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See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.