Abstract
Pokkah Boeng is a serious disease of sugarcane, which can lead to devastating yield losses in crop-producing regions, including southern China. However, there is still uncertainty about the causal agent of the disease. Our aim was to isolate and characterize the pathogen through morphological, physiological, and molecular analyses. We isolated sugarcane-colonizing fungi in Fujian, China. Isolated fungi were first assessed for their cell wall degrading enzyme capabilities, and five isolates were identified for further analysis. Internal transcribed spacer sequencing revealed that these five strains are Fusarium, Alternaria, Phoma, Phomopsis, and Epicoccum. The Fusarium isolate was further identified as F. verticillioides after Calmodulin and EF-1 gene sequencing and microscopic morphology study. Pathogenicity assay confirmed that F. verticillioides was directly responsible for disease on sugarcane. Co-inoculation of F. verticillioides with other isolated fungi did not lead to a significant difference in disease severity, refuting the idea that other cellulolytic fungi can increase disease severity as an endophyte. This is the first report characterizing pathogenic F. verticillioides on sugarcane in southern China.
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Claims 5
Claim · model-written summary
ITS sequencing identified five sugarcane-colonizing isolates as Fusarium, Alternaria, Phoma, Phomopsis and Epicoccum.
Verbatim
e1“We isolated sugarcane-colonizing fungi in Fujian, China”
e2“Internal transcribed spacer sequencing revealed that these five strains are Fusarium, Alternaria, Phoma, Phomopsis, and Epicoccum”
Claim · model-written summary
The Fusarium isolate was identified as F. verticillioides by calmodulin and EF-1 gene sequencing and microscopic morphology.
- Taxon
- sugarcanee1
- Interactor
- F. verticillioides
Fusarium verticillioidese3pathogen - Outcome
- identified as F. verticillioidese3
- Qualifier
- after Calmodulin and EF-1 gene sequencing and microscopic morphology studye3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“We isolated sugarcane-colonizing fungi in Fujian, China”
e3“The Fusarium isolate was further identified as F. verticillioides after Calmodulin and EF-1 gene sequencing and microscopic morphology study”
Claim · model-written summary
Pathogenicity assay showed F. verticillioides was directly responsible for disease on sugarcane.
- Taxon
- sugarcanee4
- Treatment
- Pathogenicity assaye4
- Interactor
- F. verticillioidesFusarium verticillioidese4pathogen
- Outcome
- diseasee4
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Pathogenicity assay confirmed that F. verticillioides was directly responsible for disease on sugarcane”
Claim · model-written summary
Co-inoculation of F. verticillioides with other isolated fungi did not significantly change disease severity, refuting that other cellulolytic fungi raise severity as endophytes.
- Taxon
- Sugarcanet0
- Treatment
- Co-inoculation of F. verticillioides with other isolated fungie5
- Interactor
- F. verticillioidesFusarium verticillioidese5pathogen
- Outcome
- disease severitye5
- Direction
- no_changeinferrede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Co-inoculation of F. verticillioides with other isolated fungi did not lead to a significant difference in disease severity”
t0“Pokkah Boeng Disease of Sugarcane”
Claim · model-written summary
This is the first report of pathogenic F. verticillioides on sugarcane in southern China.
- Taxon
- sugarcanee6
- Interactor
- F. verticillioidesFusarium verticillioidese6pathogen
- Outcome
- first report characterizing pathogenic F. verticillioidese6
- Setting
- southern Chinae6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“This is the first report characterizing pathogenic F. verticillioides on sugarcane in southern China”
The same questions in other papers
Fusarium verticillioides · disease · Saccharum officinarum (sugarcane)Results agree
1 paper in the same direction as this paper
- no effect
inoculation · disease · Saccharum officinarum (sugarcane)Positive and negative results
7 papers in the same direction as this paper · 10 in the opposite direction
- positive
- positive
- positive
14 papers more in this group
See all 18 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.