Paper · 2015

Spectrophotometric Method using Different Solvents and SPAD Chlorophyll Meter for Determination some Photosynthesis Pigments of Bean and Cowpea Plants

Ikbal Al-Barzinji; Sargul A. Khudhur; Aroul Muhson Anwar Anwar

10
claims
12
verbatim quotes
2
citations
1
research areas
—
Not open access

Abstract

In this work, some photosynthetic pigments with acetone, methanol and diethyl either were extracted spectrophotometrically, in addition to SPAD chlorophyll meter frombean (Phaseolus vulgaris L.) var. Biotack and cowpea (VignasinensisSavi) var. California black eye leaves in vegetative, flowering and podding growth stagesin Koya city, Erbil- Iraq.Results shows that concentration of chlorophyll a in bean and cowpea leaves increased significantly when acetone used in the extraction process followed by methanol and the lowest obtained when the diethyl either is used, whereas methanol was the best solvent for extracting chlorophyll b.Results also show that there were nonsignificant differences in SPAD readings between different growth stages in both of bean and cowpea plants. The data shows that the SPAD reading is high in cowpea compare to bean plants.SPAD readings correlated high significantly with chlorophylls a and b concentrations, when acetone used as solvent in cowpea plants.

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The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 10

Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Acetone extraction gave significantly increased chlorophyll a concentration in bean leaves compared with the other solvents.

Taxon
beane0
Treatment
acetonee0
Outcome
chlorophyll ae0
Direction
increasede0
Comparator
methanole0

Verbatim

e0

“concentration of chlorophyll a in bean and cowpea leaves increased significantly when acetone used in the extraction process followed by methanol and the lowest obtained when the diethyl either is used”

✓ located verbatim in the source · abstract, characters 367–568 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Acetone extraction gave significantly increased chlorophyll a concentration in cowpea leaves.

Treatment
acetonee0
Outcome
chlorophyll ae0
Direction
increasede0
Comparator
methanole0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“concentration of chlorophyll a in bean and cowpea leaves increased significantly when acetone used in the extraction process followed by methanol and the lowest obtained when the diethyl either is used”

✓ located verbatim in the source · abstract, characters 367–568 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Diethyl either yielded the lowest chlorophyll a concentration in bean leaves.

Taxon
beane0
Treatment
diethyl eithere0
Outcome
chlorophyll ae0
Direction
loweste0
Comparator
acetonee0

Verbatim

e0

“concentration of chlorophyll a in bean and cowpea leaves increased significantly when acetone used in the extraction process followed by methanol and the lowest obtained when the diethyl either is used”

✓ located verbatim in the source · abstract, characters 367–568 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Diethyl either yielded the lowest chlorophyll a concentration in cowpea leaves.

Treatment
diethyl eithere0
Outcome
chlorophyll ae0
Direction
loweste0
Comparator
acetonee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“concentration of chlorophyll a in bean and cowpea leaves increased significantly when acetone used in the extraction process followed by methanol and the lowest obtained when the diethyl either is used”

✓ located verbatim in the source · abstract, characters 367–568 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Methanol was the best solvent for chlorophyll b extraction from bean leaves.

Taxon
Beant0
Treatment
methanole0
Outcome
best solvent for extracting chlorophyll be0

Verbatim

e0

“methanol was the best solvent for extracting chlorophyll b”

✓ located verbatim in the source · abstract, characters 578–636 · show in text ↑
t0

“Bean and Cowpea Plants”

✓ located verbatim in the source · title, characters 128–150 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Methanol was the best solvent for chlorophyll b extraction from cowpea leaves.

Treatment
methanole0
Outcome
best solvent for extracting chlorophyll be0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“methanol was the best solvent for extracting chlorophyll b”

✓ located verbatim in the source · abstract, characters 578–636 · show in text ↑
t0

“Bean and Cowpea Plants”

✓ located verbatim in the source · title, characters 128–150 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

SPAD readings did not differ significantly among growth stages in bean.

Taxon
beane0
Treatment
different growth stagese0
Outcome
SPAD readingse0
Direction
nonsignificant differencese0

Not reportedComparator

Verbatim

e0

“nonsignificant differences in SPAD readings between different growth stages in both of bean and cowpea plants”

✓ located verbatim in the source · abstract, characters 671–780 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

SPAD readings did not differ significantly among growth stages in cowpea.

Treatment
different growth stagese0
Outcome
SPAD readingse0
Direction
nonsignificant differencese0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“nonsignificant differences in SPAD readings between different growth stages in both of bean and cowpea plants”

✓ located verbatim in the source · abstract, characters 671–780 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

SPAD readings were higher in cowpea than bean.

Outcome
SPAD readinge0
Direction
highe0
Comparator
beane0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“SPAD reading is high in cowpea compare to bean plants”

✓ located verbatim in the source · abstract, characters 806–859 · show in text ↑
Associationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

SPAD readings correlated significantly with chlorophyll a and b concentrations in cowpea when acetone was used.

Treatment
acetonee0
Outcome
SPAD readingse0
Qualifier
chlorophylls a and b concentrationse0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“SPAD readings correlated high significantly with chlorophylls a and b concentrations, when acetone used as solvent in cowpea plants”

✓ located verbatim in the source · abstract, characters 860–991 · show in text ↑

stage · physiology: pigments · Vigna unguiculata (cowpea)Positive and negative results

3 papers: 2 positive, 1 negative or no effect

2 in the opposite direction

  1. positive
  2. positive
See all 3 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.