Abstract
Benaty fig plant was successfully propagated by tissue culture technique and areproducible and reliable micropropagation protocol was developed through thisinvestigation. At initiation stage, healthy aseptic cultures from lateral and terminal budexplants of about 85% were achieved when the explants were treated with 0.15% HgCl 2for 7 minutes. A combined solution of ascorbic acid and citric acid (100 and 150 mgl -1 )was successfully used to overcome the problem of phenolic compounds exudation fromthe explants into the culture media. At shoot multiplication stage, the addition of 2.0 mgl -1 from both BA and kinetin gave the highest number of shoots per explant estimated at2.66 and 3.33 shoots/ explant respectively. As well as, activated charcoal promotedshoots multiplication in all multiplication parameters. The highest number of shoots perexplants (5 shoots/ explant) was obtained on MS medium containing 4.0 gl -1 AC. At rootformation stage, the interaction treatment of full MS salt strength with the use of 0.25mgl -1 NAA gave the highest rooting percentage reaching 87.5%. The highest number ofroots (18.5 roots/ explant) was recorded as well from the combined treatment of full MSsalt strength with the use of 0.25 mgl -1 NAA. While the longest roots (6.66 cm) wererecorded from the interaction treatment of half salt strength and 0.50 mgl -1 IBA. Theaddition of activated charcoal to the rooting culture medium decreased the rootingpercentage from 87.5% at control treatment to 50% and 62.5% when 2.0 and 4.0 gl -1 ACwere respectively added. The in vitro propagated fig plantlets were gradually moved in asuccessful way from lab to field conditions with 95% survival rate.
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Claims 12
Claim · model-written summary
Mercuric chloride treatment yielded about 85% healthy aseptic bud cultures.
- Taxon
- Figt0
- Material
- Benatyt0
- Organ / stage
- lateral and terminal budexplantse0
- Treatment
- 0.15% HgCl 2for 7 minutese0
- Outcome
- healthy aseptic culturese0
- Value
- about 85%e0
Not reportedComparatorDirection
Verbatim
t0“Fig (Ficus carica L. CV. Benaty)”
e0“healthy aseptic cultures from lateral and terminal budexplants of about 85% were achieved when the explants were treated with 0.15% HgCl 2for 7 minutes”
Claim · model-written summary
Ascorbic and citric acid overcame explant phenolic exudation.
- Taxon
- Figt0
- Treatment
- A combined solution of ascorbic acid and citric acid (100 and 150 mgl -1 )e0
- Outcome
- phenolic compounds exudatione0
- Direction
- overcomee0
Not reportedComparator
Verbatim
t0“Fig (Ficus carica L. CV. Benaty)”
e0“A combined solution of ascorbic acid and citric acid (100 and 150 mgl -1 )was successfully used to overcome the problem of phenolic compounds exudation fromthe explants into the culture media”
Claim · model-written summary
BA at 2.0 mgl -1 produced 2.66 shoots per explant.
- Taxon
- Figt0
- Treatment
- 2.0 mgl -1 from both BA and kinetine0
- Outcome
- number of shoots per explante0
- Direction
- higheste0
- Value
- 2.66e0
Not reportedComparator
Verbatim
t0“Fig (Ficus carica L. CV. Benaty)”
e0“the addition of 2.0 mgl -1 from both BA and kinetin gave the highest number of shoots per explant estimated at2.66 and 3.33 shoots/ explant respectively”
Claim · model-written summary
Kinetin at 2.0 mgl -1 produced 3.33 shoots per explant.
- Taxon
- Figt0
- Treatment
- 2.0 mgl -1 from both BA and kinetine0
- Outcome
- number of shoots per explante0
- Direction
- higheste0
- Value
- 3.33 shoots/ explante0
Not reportedComparator
Verbatim
t0“Fig (Ficus carica L. CV. Benaty)”
e0“the addition of 2.0 mgl -1 from both BA and kinetin gave the highest number of shoots per explant estimated at2.66 and 3.33 shoots/ explant respectively”
Claim · model-written summary
Activated charcoal promoted shoot multiplication parameters.
Not reportedComparator
Verbatim
t0“Fig (Ficus carica L. CV. Benaty)”
e0“activated charcoal promotedshoots multiplication in all multiplication parameters”
Claim · model-written summary
MS medium with 4.0 gl -1 AC gave five shoots per explant.
- Taxon
- Figt0
- Treatment
- MS medium containing 4.0 gl -1 ACe0
- Outcome
- number of shoots perexplantse0
- Direction
- higheste0
- Value
- 5 shoots/ explante0
Not reportedComparator
Verbatim
t0“Fig (Ficus carica L. CV. Benaty)”
e0“The highest number of shoots perexplants (5 shoots/ explant) was obtained on MS medium containing 4.0 gl -1 AC”
Claim · model-written summary
Full-strength MS with NAA yielded 87.5% rooting.
- Taxon
- Figt0
- Treatment
- full MS salt strength with the use of 0.25mgl -1 NAAe0
- Outcome
- rooting percentagee0
- Direction
- higheste0
- Value
- 87.5%e0
Not reportedComparator
Verbatim
t0“Fig (Ficus carica L. CV. Benaty)”
e0“full MS salt strength with the use of 0.25mgl -1 NAA gave the highest rooting percentage reaching 87.5%”
Claim · model-written summary
Full-strength MS with NAA yielded 18.5 roots per explant.
- Taxon
- Figt0
- Treatment
- full MSsalt strength with the use of 0.25 mgl -1 NAAe0
- Outcome
- number ofrootse0
- Direction
- higheste0
- Value
- 18.5 roots/ explante0
Not reportedComparator
Verbatim
t0“Fig (Ficus carica L. CV. Benaty)”
e0“The highest number ofroots (18.5 roots/ explant) was recorded as well from the combined treatment of full MSsalt strength with the use of 0.25 mgl -1 NAA”
Claim · model-written summary
Half-strength salts with IBA yielded 6.66 cm roots.
- Taxon
- Figt0
- Treatment
- half salt strength and 0.50 mgl -1 IBAe0
- Outcome
- rootse0
- Direction
- longeste0
- Value
- 6.66 cme0
Not reportedComparator
Verbatim
t0“Fig (Ficus carica L. CV. Benaty)”
e0“the longest roots (6.66 cm) wererecorded from the interaction treatment of half salt strength and 0.50 mgl -1 IBA”
Claim · model-written summary
Activated charcoal at 2.0 gl -1 lowered rooting to 50% from 87.5% control.
Verbatim
t0“Fig (Ficus carica L. CV. Benaty)”
e0“activated charcoal to the rooting culture medium decreased the rootingpercentage from 87.5% at control treatment to 50% and 62.5% when 2.0 and 4.0 gl -1 ACwere respectively added”
Claim · model-written summary
Activated charcoal at 4.0 gl -1 lowered rooting to 62.5% from 87.5% control.
Verbatim
t0“Fig (Ficus carica L. CV. Benaty)”
e0“activated charcoal to the rooting culture medium decreased the rootingpercentage from 87.5% at control treatment to 50% and 62.5% when 2.0 and 4.0 gl -1 ACwere respectively added”
Claim · model-written summary
In vitro fig plantlets survived transfer to field at 95%.
Verbatim
t0“Fig (Ficus carica L. CV. Benaty)”
e0“The in vitro propagated fig plantlets were gradually moved in asuccessful way from lab to field conditions with 95% survival rate”
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