Abstract
Plant tissue culture involves the use of explants obtained from plants to induce organogenesis with the help of plant growth regulators (PGRs). Micropropagation techniques provide a faster and economical solution to the limitations associated with traditional methods of plant cultivation. The present study focuses on the multiple shoot induction and proliferation of Ficus carica var. Black Jack. Factors that influence the growth of in vitro multiple shoots on the apical buds, which include growth media and PGRs, were investigated in this study. Different concentrations of cytokinins like 6-benzylaminopurine (BAP), Thidiazuron (TDZ), and Kinetin (Kin) were used on woody plant medium (WPM) for the optimization of media for multiple shoot induction and proliferation. Apical buds of Ficus carica var. Black Jack growing in WPM supplemented with BAP produced the healthiest plantlets, with the highest number of multiple shoots. The most efficient medium composition which produced the highest number of multiple shoots (37.8) per growing explant was WPM supplemented with 20 µM BAP. Proliferated multiple shoots were efficiently rooted using WPM + 20 µM BAP + 8 µM indole-3-acetic acid (IAA). This optimized medium composition significantly enhanced the production of multiple, disease-free plantlets using single apical bud explants of Ficus carica var. Black Jack. In the present study the observations indicate that WPM supplemented with 20 µM BAP is the best-suited medium for organogenesis and multiple shoot culture of Ficus carica var. Black Jack, and this technique can be potentially applied for commercialization of the plant. How to cite: Parab AR, Chew BL, Yeow LC, et al. Organogenesis on apical buds in common fig (Ficus carica) var. Black Jack. Electron J Biotechnol 2021;54. https://doi.org/10.1016/j.ejbt.2020.01.010
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Claims 5
Claim · model-written summary
WPM with BAP produced the healthiest fig plantlets and most multiple shoots.
- Taxon
- Ficus carica
_(Ficus_carica)_-_Cagliari%2C_Italy_2024-03-26.jpg?width=60)
Ficus caricae0 - Material
- Black Jacke0
- Organ / stage
- Apical budse0
- Treatment
- WPM supplemented with BAPe0
- Outcome
- healthiest plantlets, with the highest number of multiple shootse0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_(Ficus_carica)_-_Cagliari%2C_Italy_2024-03-26.jpg?width=500)
Verbatim
e0“Apical buds of Ficus carica var. Black Jack growing in WPM supplemented with BAP produced the healthiest plantlets, with the highest number of multiple shoots.”
Claim · model-written summary
WPM with 20 µM BAP gave 37.8 shoots per growing explant, the highest number.
- Taxon
- Ficus caricaFicus caricat0
- Treatment
- WPM supplemented with 20 µM BAPe0
- Outcome
- number of multiple shootse0
- Direction
- higheste0
- Value
- 37.8e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The most efficient medium composition which produced the highest number of multiple shoots (37.8) per growing explant was WPM supplemented with 20 µM BAP.”
t0“Ficus carica”
Claim · model-written summary
WPM with 20 µM BAP and 8 µM IAA rooted proliferated shoots efficiently.
- Taxon
- Ficus caricaFicus caricat0
- Treatment
- WPM + 20 µM BAP + 8 µM indole-3-acetic acid (IAA)e0
- Outcome
- Proliferated multiple shoots were efficiently rootede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Proliferated multiple shoots were efficiently rooted using WPM + 20 µM BAP + 8 µM indole-3-acetic acid (IAA).”
t0“Ficus carica”
Claim · model-written summary
The optimized medium significantly enhanced production of multiple disease-free fig plantlets from single apical buds.
- Taxon
- Ficus caricaFicus caricae0
- Material
- Black Jacke0
- Treatment
- optimized medium compositione0
- Outcome
- production of multiple, disease-free plantletse0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This optimized medium composition significantly enhanced the production of multiple, disease-free plantlets using single apical bud explants of Ficus carica var. Black Jack.”
Claim · model-written summary
The authors consider WPM with 20 µM BAP best suited for fig organogenesis and multiple shoot culture.
- Taxon
- Ficus caricaFicus caricae0
- Material
- Black Jacke0
- Treatment
- WPM supplemented with 20 µM BAPe0
- Outcome
- best-suited medium for organogenesis and multiple shoot culturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“WPM supplemented with 20 µM BAP is the best-suited medium for organogenesis and multiple shoot culture of Ficus carica var. Black Jack”
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