Abstract
The present investigation was carried out for the induction of haploid plants in Citrus grandis through in situ parthenogenesis by pollination with gamma-irradiated pollen of C. limetta and C. sinensis, treated with 50, 100, 200, 300, and 400 Gy gamma ray doses followed by in vitro ovule culture. Ovule culture at 50 days after pollination (DAP) was found optimum for the maximum recovery of in vitro-raised plantlets as compared to 35 and 20 DAP. At 50 DAP, irrespective of the pollen parent, plantlet regeneration capacity decreased at 300 and 400 Gy irradiation treatment (0.62% and 0.60%, respectively) with maximum recovery in the nonirradiated control (3.07%). Chromosome counting in actively growing root tips of all the in vitro-raised ovule cultured plantlets revealed that two haploid plants with chromosome number of nine were induced in C. grandis from the ovule culture that was cultured at 50 DAP following pollination with irradiated pollen of C. sinensis at 400 Gy and C. limetta pollen at 300 Gy. However, irradiation doses below 300 Gy were incapable of inducing any haploids in either cross combination. Similarly, all the plantlets regenerated from in vitro embryo culture of mature seeds were found to be diploid in nature, irrespective of the pollen parent and irradiation dose. Molecular analysis of the in vitro-raised haploid and diploid plants using SSR markers confirmed the maternal origins of the haploid plants; however, the diploid plants were found zygotic in nature with one allele from the seed parent and the other one from the pollen parent.
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Claims 9
Claim · model-written summary
Ovule culture at 50 days after pollination maximized plantlet recovery relative to 35 and 20 days.
- Treatment
- 50 days after pollination (DAP)e0
- Outcome
- recovery of in vitro-raised plantletse0
- Direction
- maximume0
- Comparator
- 35 and 20 DAPe0
CitrusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ovule culture at 50 days after pollination (DAP) was found optimum for the maximum recovery of in vitro-raised plantlets as compared to 35 and 20 DAP”
t0“citrus”
Claim · model-written summary
At 50 DAP, 300 Gy irradiation reduced plantlet regeneration to 0.62% versus 3.07% in controls.
- Treatment
- 300e0
- Outcome
- plantlet regeneration capacitye0
- Direction
- decreasede0
- Value
- 0.62%e0
- Qualifier
- At 50 DAPe0
- Comparator
- nonirradiated controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At 50 DAP, irrespective of the pollen parent, plantlet regeneration capacity decreased at 300 and 400 Gy irradiation treatment (0.62% and 0.60%, respectively) with maximum recovery in the nonirradiated control (3.07%)”
t0“citrus”
Claim · model-written summary
At 50 DAP, 400 Gy irradiation reduced plantlet regeneration to 0.60% versus 3.07% in controls.
- Treatment
- 400 Gy irradiation treatmente0
- Outcome
- plantlet regeneration capacitye0
- Direction
- decreasede0
- Value
- 0.60%e0
- Qualifier
- At 50 DAPe0
- Comparator
- nonirradiated controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At 50 DAP, irrespective of the pollen parent, plantlet regeneration capacity decreased at 300 and 400 Gy irradiation treatment (0.62% and 0.60%, respectively) with maximum recovery in the nonirradiated control (3.07%)”
t0“citrus”
Claim · model-written summary
Irradiated C. sinensis pollen at 400 Gy yielded a haploid C. grandis plant.
- Taxon
- C. grandis
Citrus maximae0 - Treatment
- irradiated pollen of C. sinensis at 400 Gye0
- Outcome
- haploid plantse0
- Value
- ninee0
- Qualifier
- 50 DAPe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two haploid plants with chromosome number of nine were induced in C. grandis from the ovule culture that was cultured at 50 DAP following pollination with irradiated pollen of C. sinensis at 400 Gy and C. limetta pollen at 300 Gy”
Claim · model-written summary
Irradiated C. limetta pollen at 300 Gy yielded a haploid C. grandis plant.
- Taxon
- C. grandisCitrus maximae0
- Treatment
- C. limetta pollen at 300 Gye0
- Outcome
- haploid plantse0
- Value
- ninee0
- Qualifier
- 50 DAPe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two haploid plants with chromosome number of nine were induced in C. grandis from the ovule culture that was cultured at 50 DAP following pollination with irradiated pollen of C. sinensis at 400 Gy and C. limetta pollen at 300 Gy”
Claim · model-written summary
Irradiation below 300 Gy yielded no haploids in either cross.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“irradiation doses below 300 Gy were incapable of inducing any haploids in either cross combination”
t0“citrus”
Claim · model-written summary
All plantlets from mature-seed embryo culture were diploid.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all the plantlets regenerated from in vitro embryo culture of mature seeds were found to be diploid in nature, irrespective of the pollen parent and irradiation dose”
t0“citrus”
Claim · model-written summary
SSR analysis confirmed maternal origins of haploid plants.
- Outcome
- maternal origins of the haploid plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Molecular analysis of the in vitro-raised haploid and diploid plants using SSR markers confirmed the maternal origins of the haploid plants”
t0“citrus”
Claim · model-written summary
SSR analysis found diploid plants to be zygotic, with alleles from both parents.
- Outcome
- zygotic in naturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the diploid plants were found zygotic in nature with one allele from the seed parent and the other one from the pollen parent”
t0“citrus”
The same questions in other papers
radiation · growth · CitrusResults agree
2 papers in the same direction as this paper
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.