Paper · 1989 · Journal of the Japanese Society for Horticultural Science

Effects of ^|^gamma;-Irradiation on Growth and Embryogenesis of Citrus Callus

Nobumasa NITO; Jing-Tian LING; Masao IWAMASA; et al.

17
claims
19
verbatim quotes
3
citations
2
research areas
✓
Open access

Abstract

The embryogenic callus of ‘Valencia’ orange, ‘Yoshida’ navel orange (Citrus sinensis (L.) Osbeck), Calamondin (C. madurensis Lour.), Yuzu (C. junos Sieb. ex Tan.) and ‘Ishizuka Wase’ satsuma (C. unshiu Marc.) were exposed to 1, 5, 10, 20 and 50 kiloroentgen (kR) of gamma irradiation at the dosage of 1kR/h in Institute of Radiation Breeding, Ministry of Agriculture, Forestry and Fishery, Japan.The irradiation caused a retardation of callus growth. Callus growth was reduced to 500 or less of that in non-irradiated callus, at a dosage of 5kR in Yuzu, 20kR in ‘Valencia’ orange and ‘Ishizuka Wase’ satsuma, and 50kR in ‘Yoshida’ navel orange and Calamondin.The irradiation stimulated embryoid formation from the callus. Optimum irradiation dosages to produce the maximum number of embryoids were 50kR in ‘Valencia’ orange, 20kR in Yuzu, 10kR in Calamondin and ‘Ishizuka Wase’ satsuma. ‘Yoshida’ navel orange produced the maximum number of embryoids at the lowest irradiation of 5kR.The ratios of embryoid number produced at the optimum irradiation to that at non- irradiation were, 16.4, 8.4, 7.8, 6.4 and 2.7 in Yuzu, ‘Ishizuka Wase’ satsuma, ‘Valencia’ orange, ‘Yoshida’ navel orange and Calamondin, respectively.

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Claims 17

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Irradiation retarded citrus callus growth.

Taxon
CitrusCitrust0
Treatment
irradiatione0
Outcome
callus growthe0
Direction
retardatione0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus
Citrus · Taxon · picture of the genus (one of its species)Ellen Levy Finch ( Elf ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The irradiation caused a retardation of callus growth.”

✓ located verbatim in the source · abstract, characters 396–450 · show in text ↑
t0

“Citrus”

✓ located verbatim in the source · title, characters 64–70 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Yuzu callus growth was reduced at 5kR.

Treatment
5kRe0
Outcome
Callus growthe0
Direction
reducede0
Value
500 or lesse0
Comparator
non-irradiated calluse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus junos
Citrus junos · TaxonNikita from Russian Federation · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“Callus growth was reduced to 500 or less of that in non-irradiated callus, at a dosage of 5kR in Yuzu”

✓ located verbatim in the source · abstract, characters 451–552 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Valencia orange callus growth was reduced at 20kR.

Taxon
‘Valencia’ orangee0
Treatment
20kRe0
Outcome
Callus growthe0
Direction
reducede0
Comparator
non-irradiated calluse0

Verbatim

e0

“Callus growth was reduced to 500 or less of that in non-irradiated callus, at a dosage of 5kR in Yuzu, 20kR in ‘Valencia’ orange”

✓ located verbatim in the source · abstract, characters 451–579 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Ishizuka Wase satsuma callus growth was reduced at 20kR.

Taxon
‘Ishizuka Wase’ satsumae0
Treatment
20kRe0
Outcome
Callus growthe0
Direction
reducede0
Comparator
non-irradiated calluse0

Verbatim

e0

“Callus growth was reduced to 500 or less of that in non-irradiated callus, at a dosage of 5kR in Yuzu, 20kR in ‘Valencia’ orange and ‘Ishizuka Wase’ satsuma”

✓ located verbatim in the source · abstract, characters 451–607 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Yoshida navel orange callus growth was reduced at 50kR.

Taxon
‘Yoshida’ navel orangee0
Treatment
50kRe0
Outcome
Callus growthe0
Direction
reducede0
Comparator
non-irradiated calluse0

Verbatim

e0

“Callus growth was reduced to 500 or less of that in non-irradiated callus, at a dosage of 5kR in Yuzu, 20kR in ‘Valencia’ orange and ‘Ishizuka Wase’ satsuma, and 50kR in ‘Yoshida’ navel orange”

✓ located verbatim in the source · abstract, characters 451–643 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Calamondin callus growth was reduced at 50kR.

Taxon
CalamondinCitrus ×microcarpae0
Treatment
50kRe0
Outcome
Callus growthe0
Direction
reducede0
Comparator
non-irradiated calluse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus x microcarpa
Citrus ×microcarpa · TaxonKrzysztof Ziarnek, Kenraiz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Callus growth was reduced to 500 or less of that in non-irradiated callus, at a dosage of 5kR in Yuzu, 20kR in ‘Valencia’ orange and ‘Ishizuka Wase’ satsuma, and 50kR in ‘Yoshida’ navel orange and Calamondin”

✓ located verbatim in the source · abstract, characters 451–658 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Irradiation stimulated embryoid formation.

Taxon
CitrusCitrust0
Treatment
irradiatione0
Outcome
embryoid formatione0
Direction
stimulatede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus
Citrus · Taxon · picture of the genus (one of its species)Ellen Levy Finch ( Elf ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The irradiation stimulated embryoid formation from the callus.”

✓ located verbatim in the source · abstract, characters 659–721 · show in text ↑
t0

“Citrus”

✓ located verbatim in the source · title, characters 64–70 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Valencia orange had maximal embryoid production at 50kR.

Taxon
‘Valencia’ orangee0
Treatment
50kRe0
Outcome
number of embryoidse0
Value
50kRe0

Verbatim

e0

“Optimum irradiation dosages to produce the maximum number of embryoids were 50kR in ‘Valencia’ orange”

✓ located verbatim in the source · abstract, characters 722–823 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Yuzu had maximal embryoid production at 20kR.

Treatment
20kRe0
Outcome
number of embryoidse0
Value
20kRe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus junos
Citrus junos · TaxonNikita from Russian Federation · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“Optimum irradiation dosages to produce the maximum number of embryoids were 50kR in ‘Valencia’ orange, 20kR in Yuzu”

✓ located verbatim in the source · abstract, characters 722–837 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Calamondin had maximal embryoid production at 10kR.

Taxon
CalamondinCitrus ×microcarpae0
Treatment
10kRe0
Outcome
number of embryoidse0
Value
10kRe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus x microcarpa
Citrus ×microcarpa · TaxonKrzysztof Ziarnek, Kenraiz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Optimum irradiation dosages to produce the maximum number of embryoids were 50kR in ‘Valencia’ orange, 20kR in Yuzu, 10kR in Calamondin”

✓ located verbatim in the source · abstract, characters 722–857 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Ishizuka Wase satsuma had maximal embryoid production at 10kR.

Taxon
‘Ishizuka Wase’ satsumae0
Treatment
10kRe0
Outcome
number of embryoidse0
Value
10kRe0

Verbatim

e0

“Optimum irradiation dosages to produce the maximum number of embryoids were 50kR in ‘Valencia’ orange, 20kR in Yuzu, 10kR in Calamondin and ‘Ishizuka Wase’ satsuma”

✓ located verbatim in the source · abstract, characters 722–885 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Yoshida navel orange had maximal embryoid production at 5kR.

Taxon
‘Yoshida’ navel orangee0
Treatment
irradiation of 5kRe0
Outcome
number of embryoidse0
Direction
maximume0
Value
5kRe0

Verbatim

e0

“‘Yoshida’ navel orange produced the maximum number of embryoids at the lowest irradiation of 5kR.”

✓ located verbatim in the source · abstract, characters 887–984 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Yuzu had a 16.4 optimum-to-control embryoid count ratio.

Outcome
embryoid numbere0
Value
16.4e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus junos
Citrus junos · TaxonNikita from Russian Federation · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“The ratios of embryoid number produced at the optimum irradiation to that at non- irradiation were, 16.4, 8.4, 7.8, 6.4 and 2.7 in Yuzu”

✓ located verbatim in the source · abstract, characters 984–1119 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Ishizuka Wase satsuma had an 8.4 optimum-to-control embryoid count ratio.

Taxon
‘Ishizuka Wase’ satsumae0
Outcome
embryoid numbere0
Value
8.4e0

Verbatim

e0

“The ratios of embryoid number produced at the optimum irradiation to that at non- irradiation were, 16.4, 8.4, 7.8, 6.4 and 2.7 in Yuzu, ‘Ishizuka Wase’ satsuma”

✓ located verbatim in the source · abstract, characters 984–1144 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Valencia orange had a 7.8 optimum-to-control embryoid count ratio.

Taxon
‘Valencia’ orangee0
Outcome
embryoid numbere0
Value
7.8e0

Verbatim

e0

“The ratios of embryoid number produced at the optimum irradiation to that at non- irradiation were, 16.4, 8.4, 7.8, 6.4 and 2.7 in Yuzu, ‘Ishizuka Wase’ satsuma, ‘Valencia’ orange”

✓ located verbatim in the source · abstract, characters 984–1163 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Yoshida navel orange had a 6.4 optimum-to-control embryoid count ratio.

Taxon
‘Yoshida’ navel orangee0
Outcome
embryoid numbere0
Value
6.4e0

Verbatim

e0

“The ratios of embryoid number produced at the optimum irradiation to that at non- irradiation were, 16.4, 8.4, 7.8, 6.4 and 2.7 in Yuzu, ‘Ishizuka Wase’ satsuma, ‘Valencia’ orange, ‘Yoshida’ navel orange”

✓ located verbatim in the source · abstract, characters 984–1187 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Calamondin had a 2.7 optimum-to-control embryoid count ratio.

Taxon
CalamondinCitrus ×microcarpae0
Outcome
embryoid numbere0
Value
2.7e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus x microcarpa
Citrus ×microcarpa · TaxonKrzysztof Ziarnek, Kenraiz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The ratios of embryoid number produced at the optimum irradiation to that at non- irradiation were, 16.4, 8.4, 7.8, 6.4 and 2.7 in Yuzu, ‘Ishizuka Wase’ satsuma, ‘Valencia’ orange, ‘Yoshida’ navel orange and Calamondin”

✓ located verbatim in the source · abstract, characters 984–1202 · show in text ↑

radiation · growth · CitrusResults agree

3 papers: 0 positive, 3 negative or no effect

2 papers in the same direction as this paper

  1. negative
  2. negative
See all 3 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.