Abstract
The tomato Tobacco mosaic virus resistance-22 (Tm-22) gene encodes a coiled-coil-nucleotide binding site-Leu-rich repeat protein lacking a conventional plasma membrane (PM) localization motif. Tm-22 confers plant extreme resistance against tobamoviruses including Tobacco mosaic virus (TMV) by recognizing the avirulence (Avr) viral movement protein (MP). However, the subcellular compartment where Tm-22 functions is unclear. Here, we demonstrate that Tm-22 interacts with TMV MP to form a protein complex at the PM. We show that both inactive and active Tm-22 proteins are localized to the PM. When restricted to PM by fusing Tm-22 to the S-acylated PM association motif, the Tm-22 fusion protein can still induce a hypersensitive response cell death, consistent with its activation at the PM. Through analyses of viral MP mutants, we find that the plasmodesmata (PD) localization of the Avr protein MP is not required for Tm-22 function. These results suggest that Tm-22-mediated resistance takes place on PM without requirement of its Avr protein to be located to PD.
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Claims 5
Claim · model-written summary
Tm-22 forms a complex with TMV movement protein at the plasma membrane.
- Taxon
- tomato
Solanum lycopersicume1 - Interactor
- TMV
Tobacco mosaic viruse0pathogen - Gene
- Tm-22e0
- Outcome
- protein complex at the PMe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tm-22 interacts with TMV MP to form a protein complex at the PM”
e1“tomato Tobacco mosaic virus”
Claim · model-written summary
Inactive and active Tm-22 proteins localized to the plasma membrane.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- Tm-22e0
- Outcome
- localized to the PMe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both inactive and active Tm-22 proteins are localized to the PM”
e1“tomato Tobacco mosaic virus”
Claim · model-written summary
PM-restricted Tm-22 still induced hypersensitive cell death.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- Tm-22 fusion proteine0
- Gene
- Tm-22e0
- Outcome
- hypersensitive response cell deathe0
- Direction
- inducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the Tm-22 fusion protein can still induce a hypersensitive response cell death”
e1“tomato Tobacco mosaic virus”
Claim · model-written summary
Movement-protein plasmodesmata localization was unnecessary for Tm-22 function.
- Taxon
- tomatoSolanum lycopersicume1
- Interactor
- Tobacco mosaic virusTobacco mosaic viruse1pathogen
- Gene
- Tm-22e0
- Outcome
- Tm-22 functione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plasmodesmata (PD) localization of the Avr protein MP is not required for Tm-22 function”
e1“tomato Tobacco mosaic virus”
Claim · model-written summary
Authors infer Tm-22 resistance operates at the plasma membrane.
- Taxon
- tomatoSolanum lycopersicume1
- Interactor
- Tobacco mosaic virusTobacco mosaic viruse1pathogen
- Gene
- Tm-22e0
- Outcome
- Tm-22-mediated resistancee0
- Qualifier
- on PMe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tm-22-mediated resistance takes place on PM without requirement of its Avr protein to be located to PD”
e1“tomato Tobacco mosaic virus”
The same questions in other papers
Nicotiana tabacum · disease · Solanum lycopersicum (tomato)Positive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
- no effect
- no effect
- negative
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.