Abstract
A study was conducted at the Tissue Culture Laboratory of ICABIOGRAD, Bogor, to obtain an optimum medium formulation for calli regenerations of for rice varities (Ciherang, Cisadane, IR64, and T-309). The research activities were done in five steps, i.e., callus induction, callus regeneration, shoot multiplication, root formation, and plant acclimatization. The type of explants used in the study was embriozygotic explants. Five media formulations were used for the callus induction, while four media formulations were used for the callus regeneration. The results showed that the best medium formulation for induction of callus formation was MS + 2,4-D 2 mg/l + casein hidrolisat 3 mg/l, while the best medium formulation for callus regeneration was MS + BA 3 mg/l + thidiazuron 0,1 mg/l.
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Claims 2
Claim · model-written summary
The best medium for callus induction in the four rice varieties was MS + 2,4-D 2 mg/l + casein hidrolisat 3 mg/l.
- Taxon
- rice
Oryza sativae1 - Material
- Ciherang, Cisadane, IR64, and T-309e1
- Treatment
- MS + 2,4-D 2 mg/l + casein hidrolisat 3 mg/le2
- Outcome
- callus formatione2
- Direction
- beste2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“calli regenerations of for rice varities (Ciherang, Cisadane, IR64, and T-309)”
e2“the best medium formulation for induction of callus formation was MS + 2,4-D 2 mg/l + casein hidrolisat 3 mg/l”
Claim · model-written summary
The best medium for callus regeneration in the four rice varieties was MS + BA 3 mg/l + thidiazuron 0,1 mg/l.
- Taxon
- riceOryza sativae1
- Material
- Ciherang, Cisadane, IR64, and T-309e1
- Treatment
- MS + BA 3 mg/l + thidiazuron 0,1 mg/le3
- Outcome
- callus regeneratione3
- Direction
- beste3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“calli regenerations of for rice varities (Ciherang, Cisadane, IR64, and T-309)”
e3“the best medium formulation for callus regeneration was MS + BA 3 mg/l + thidiazuron 0,1 mg/l”
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N-benzyladenine · growth · Oryza sativa (rice)Results differ
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- positive
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See all 72 papers →2,4-D · growth · Oryza sativa (rice)Positive and negative results
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See all 107 papers →Thidiazuron · growth · Oryza sativa (rice)Results agree
2 papers in the same direction as this paper
- positive
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.