Abstract
Tomato yellow leaf curl virus (TYLCV) is a single-stranded (ssDNA) begomoviruses that causes severe damage to tomato and several other crops worldwide. TYLCV is exclusively transmitted by the sweetpotato whitefly, Bemisia tabaci in a persistent circulative and propagative manner. Previous studies have shown that the transmission, retention and circulation of TYLCV in its vector involves interaction with insect and endosymbiont proteins, which aid in the transmission of the virus, or have a protective role in response to the presence of the virus in the insect body. However, only a low number of such proteins have been identified. Here, the role of B. tabaci Cyclophilin B (CypB) in the transmission of TYLCV protein was investigated. Cyclophilins (Cyps) are a large family of cellular prolyl isomerases that have many molecular roles including facilitating protein-protein interactions in the cell. One cyclophilin protein has been implicated in aphid-luteovirus interactions. We demonstrate that the expression of CypB from B. tabaci is altered upon TYLCV acquisition and retention. Further experiments used immunocapture-PCR and co-immunolocalization and demonstrated a specific interaction and colocalization between CypB and TYLCV in the the midgut, eggs and salivary glands. Membrane feeding of anti-CypB antibodies and TYLCV infected plants showed a decrease in TYLCV transmission, suggesting a critical role that CypB plays in TYLCV transmission. Further experiments, which used membrane feeding with the CypB inhibitor Cyclosporin A (CsA) showed decrease in CypB-TYLCV colocalization in the midgut and virus transmission. Altogether, our results indicate that CypB plays an important role in TYLCV transmission by B. tabaci.
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Claims 5
Claim · model-written summary
Expression of B. tabaci CypB is altered upon TYLCV acquisition and retention.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- TYLCV acquisition and retentione1
- Interactor
- B. tabaci
Bemisia tabacie1pest - Second interactor
- TYLCVTomato yellow leaf curl viruse1pathogen
- Gene
- CypBe1
- Outcome
- expression of CypBe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“causes severe damage to tomato and several other crops worldwide”
e1“We demonstrate that the expression of CypB from B. tabaci is altered upon TYLCV acquisition and retention”
Claim · model-written summary
CypB specifically interacts and colocalizes with TYLCV in the midgut, eggs and salivary glands of B. tabaci.
- Taxon
- tomatoSolanum lycopersicume0
- Organ / stage
- midgut, eggs and salivary glandse2
- Interactor
- TYLCVTomato yellow leaf curl viruse2pathogen
- Gene
- CypBe2
- Outcome
- interaction and colocalizatione2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“causes severe damage to tomato and several other crops worldwide”
e2“demonstrated a specific interaction and colocalization between CypB and TYLCV in the the midgut, eggs and salivary glands”
Claim · model-written summary
Membrane feeding of anti-CypB antibodies decreases TYLCV transmission to tomato.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- Membrane feeding of anti-CypB antibodiese3
- Interactor
- TYLCVTomato yellow leaf curl viruse3pathogen
- Gene
- CypBe3
- Outcome
- TYLCV transmissione3
- Direction
- decreasee3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“causes severe damage to tomato and several other crops worldwide”
e3“Membrane feeding of anti-CypB antibodies and TYLCV infected plants showed a decrease in TYLCV transmission”
Claim · model-written summary
Cyclosporin A membrane feeding decreases CypB-TYLCV colocalization in the midgut.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- membrane feeding with the CypB inhibitor Cyclosporin A (CsA)e4
- Interactor
- TYLCVTomato yellow leaf curl viruse4pathogen
- Gene
- CypBe4
- Outcome
- CypB-TYLCV colocalization in the midgute4
- Direction
- decreasee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“causes severe damage to tomato and several other crops worldwide”
e4“Further experiments, which used membrane feeding with the CypB inhibitor Cyclosporin A (CsA) showed decrease in CypB-TYLCV colocalization in the midgut”
Claim · model-written summary
Cyclosporin A membrane feeding decreases TYLCV transmission.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- membrane feeding with the CypB inhibitor Cyclosporin A (CsA)e5
- Gene
- CypBe5
- Outcome
- virus transmissione5
- Direction
- decreasee5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“causes severe damage to tomato and several other crops worldwide”
e5“Further experiments, which used membrane feeding with the CypB inhibitor Cyclosporin A (CsA) showed decrease in CypB-TYLCV colocalization in the midgut and virus transmission”
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See all 56 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.