Abstract
Naturally occurring auxin (IAA) was measured spectrophotometrically as indicator for oxidative processes that occurs during ageing phenomenon, in terms of rooting response of mung bean cuttings. According to oxidative hypothesis fresh and aged cuttings as well as stock plants were supplied with nutrient solutions that are associated with anti-oxidant defense mechanisms, to investigate their effects in suppression of oxidative stress that accompanied aging phenomenon. The data revealed the followings:a) A decline in rooting response of aged cuttings (held in d/H2O for 3 days) were taken from seedlings grown in Hoagland solution compared to fresh cuttings, with a percentage of decline equal to 11.8% in presence of NAA, 10 -4 M and 30.8% in its absence. This coincided with the decline of IAA level in aged cuttings compared to fresh cuttings, and was attributed to oxidative processes that occurs during ageing. b) A decline in rooting response of aged cuttings in d/H2O (supplied with auxin) taken from seedlings grown in Hoagland solutions lacking for B or Fe, compared to control(complete Hoagland solution). c) Significant increase in rooting response of cuttings aged in d/H2O (not supplied with auxin) taken from seedlings grown in Hoagland solution lacking for Mn or B. d) Increase in rooting response of fresh cuttings treated with low concentration of SeO2 (0.001-10 ppm). e) Increase in rooting response of cuttings aged in different strengths of complete, modified nutrient solution (CMNS), except the case of CMNS (SeO2=1 ppm). This confirmed the role of selenium as anti-oxidant in defense mechanisms occurring in cuttings during ageing. f) Increase in rootings response of cuttings aged in d/H2O (not supplied with auxin), taken from seedlings grown in CMNS lacking for boron ( In this case, B substituted by Se). g) Decrease in rooting response of cuttings taken from seedlings grown in d/H2O for (10) days, and aged in CMNS (Half strength) lacking for Mn or group of elements (Mn, Zn, and Se). The discussion was focused on the importan ce of trace elements
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Claims 12
Claim · model-written summary
Aged cuttings supplied with NAA had 11.8% less rooting than fresh cuttings.
- Taxon
- Mung bean
.jpg?width=60)
Vigna radiatat0 - Organ / stage
- cuttingse0
- Treatment
- aged cuttings (held in d/H2O for 3 days)e0
- Outcome
- rooting responsee0
- Direction
- declinee0
- Value
- 11.8%e0
- Qualifier
- in presence of NAA, 10 -4 Me0
- Comparator
- fresh cuttingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“Mung bean (Phaseolus aureus)”
e0“A decline in rooting response of aged cuttings (held in d/H2O for 3 days) were taken from seedlings grown in Hoagland solution compared to fresh cuttings, with a percentage of decline equal to 11.8% in presence of NAA, 10 -4 M and 30.8% in its absence”
Claim · model-written summary
Aged cuttings without NAA had 30.8% less rooting than fresh cuttings.
- Taxon
- Mung beanVigna radiatat0
- Organ / stage
- cuttingse0
- Treatment
- aged cuttings (held in d/H2O for 3 days)e0
- Outcome
- rooting responsee0
- Direction
- declinee0
- Value
- 30.8%e0
- Qualifier
- in its absencee0
- Comparator
- fresh cuttingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mung bean (Phaseolus aureus)”
e0“A decline in rooting response of aged cuttings (held in d/H2O for 3 days) were taken from seedlings grown in Hoagland solution compared to fresh cuttings, with a percentage of decline equal to 11.8% in presence of NAA, 10 -4 M and 30.8% in its absence”
Claim · model-written summary
IAA level declined in aged cuttings compared with fresh cuttings.
- Taxon
- Mung beanVigna radiatat0
- Organ / stage
- aged cuttingse0
- Outcome
- IAA levele0
- Direction
- declinee0
- Comparator
- fresh cuttingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mung bean (Phaseolus aureus)”
e0“This coincided with the decline of IAA level in aged cuttings compared to fresh cuttings”
Claim · model-written summary
Aged cuttings from seedlings grown without B had less rooting than controls.
- Taxon
- Mung beanVigna radiatat0
- Organ / stage
- aged cuttingse0
- Treatment
- Hoagland solutions lacking for Be0
- Outcome
- rooting responsee0
- Direction
- declinee0
- Comparator
- control(complete Hoagland solution)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mung bean (Phaseolus aureus)”
e0“A decline in rooting response of aged cuttings in d/H2O (supplied with auxin) taken from seedlings grown in Hoagland solutions lacking for B or Fe, compared to control(complete Hoagland solution)”
Claim · model-written summary
Aged cuttings from seedlings grown without Fe had less rooting than controls.
- Taxon
- Mung beanVigna radiatat0
- Organ / stage
- aged cuttingse0
- Treatment
- Fee0
- Outcome
- rooting responsee0
- Direction
- declinee0
- Comparator
- control(complete Hoagland solution)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mung bean (Phaseolus aureus)”
e0“A decline in rooting response of aged cuttings in d/H2O (supplied with auxin) taken from seedlings grown in Hoagland solutions lacking for B or Fe, compared to control(complete Hoagland solution)”
Claim · model-written summary
Without auxin, aged cuttings from Mn-deficient seedlings had significantly greater rooting.
- Taxon
- Mung beanVigna radiatat0
- Organ / stage
- cuttingse0
- Treatment
- Hoagland solution lacking for Mne0
- Outcome
- rooting responsee0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mung bean (Phaseolus aureus)”
e0“Significant increase in rooting response of cuttings aged in d/H2O (not supplied with auxin) taken from seedlings grown in Hoagland solution lacking for Mn or B”
Claim · model-written summary
Without auxin, aged cuttings from B-deficient seedlings had significantly greater rooting.
- Taxon
- Mung beanVigna radiatat0
- Organ / stage
- cuttingse0
- Treatment
- Be0
- Outcome
- rooting responsee0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mung bean (Phaseolus aureus)”
e0“Significant increase in rooting response of cuttings aged in d/H2O (not supplied with auxin) taken from seedlings grown in Hoagland solution lacking for Mn or B”
Claim · model-written summary
Low SeO2 concentration increased rooting of fresh cuttings.
- Taxon
- Mung beanVigna radiatat0
- Organ / stage
- fresh cuttingse0
- Treatment
- low concentration of SeO2 (0.001-10 ppm)e0
- Outcome
- rooting responsee0
- Direction
- Increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mung bean (Phaseolus aureus)”
e0“Increase in rooting response of fresh cuttings treated with low concentration of SeO2 (0.001-10 ppm)”
Claim · model-written summary
Ageing in strengths of complete modified nutrient solution increased rooting, except at SeO2=1 ppm.
- Taxon
- Mung beanVigna radiatat0
- Treatment
- different strengths of complete, modified nutrient solution (CMNS)e0
- Outcome
- rooting responsee0
- Direction
- Increasee0
- Qualifier
- except the case of CMNS (SeO2=1 ppm)e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mung bean (Phaseolus aureus)”
e0“Increase in rooting response of cuttings aged in different strengths of complete, modified nutrient solution (CMNS), except the case of CMNS (SeO2=1 ppm)”
Claim · model-written summary
B substitution by Se increased rooting of aged cuttings without auxin.
- Taxon
- Mung beanVigna radiatat0
- Treatment
- CMNS lacking for borone0
- Outcome
- rootings responsee0
- Direction
- Increasee0
- Qualifier
- B substituted by See0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mung bean (Phaseolus aureus)”
e0“Increase in rootings response of cuttings aged in d/H2O (not supplied with auxin), taken from seedlings grown in CMNS lacking for boron ( In this case, B substituted by Se)”
Claim · model-written summary
Mn omission from half-strength CMNS reduced rooting.
- Taxon
- Mung beanVigna radiatat0
- Treatment
- CMNS (Half strength) lacking for Mne0
- Outcome
- rooting responsee0
- Direction
- Decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mung bean (Phaseolus aureus)”
e0“Decrease in rooting response of cuttings taken from seedlings grown in d/H2O for (10) days, and aged in CMNS (Half strength) lacking for Mn or group of elements (Mn, Zn, and Se)”
Claim · model-written summary
Omission of Mn, Zn, and Se from half-strength CMNS reduced rooting.
- Taxon
- Mung beanVigna radiatat0
- Treatment
- group of elements (Mn, Zn, and Se)e0
- Outcome
- rooting responsee0
- Direction
- Decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mung bean (Phaseolus aureus)”
e0“Decrease in rooting response of cuttings taken from seedlings grown in d/H2O for (10) days, and aged in CMNS (Half strength) lacking for Mn or group of elements (Mn, Zn, and Se)”
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