Abstract
Phototropism is the process by which plants grow towards light in order to maximize the capture of light for photosynthesis, which is particularly important for germinating seedlings. In Arabidopsis, hypocotyl phototropism is predominantly triggered by blue light (BL), which has a profound effect on the establishment of asymmetric auxin distribution, essential for hypocotyl phototropism. Two auxin efflux transporters ATP-binding cassette B19 (ABCB19) and PIN-formed 3 (PIN3) are known to mediate the effect of BL on auxin distribution in the hypocotyl, but the details for how BL triggers PIN3 lateralization remain poorly understood. Here, we report a critical role for clathrin in BL-triggered, PIN3-mediated asymmetric auxin distribution in hypocotyl phototropism. We show that unilateral BL induces relocalization of clathrin in the hypocotyl. Loss of clathrin light chain 2 (CLC2) and CLC3 affects endocytosis and lateral distribution of PIN3 thereby impairing BL-triggered establishment of asymmetric auxin distribution and consequently, phototropic bending. Conversely, auxin efflux inhibitors N-1-naphthylphthalamic acid and 2,3,5-triiodobenzoic acid affect BL-induced relocalization of clathrin, endocytosis and lateralization of PIN3 as well as asymmetric distribution of auxin. These results together demonstrate an important interplay between auxin and clathrin function that dynamically regulates BL-triggered hypocotyl phototropism in Arabidopsis.
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Claims 4
Claim · model-written summary
Unilateral blue light relocalized clathrin in the Arabidopsis hypocotyl.
- Taxon
- Arabidopsis
Arabidopsist0 - Organ / stage
- hypocotyle0
- Gene
- clathrine0
- Outcome
- relocalization of clathrine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“unilateral BL induces relocalization of clathrin in the hypocotyl”
t0“Arabidopsis”
Claim · model-written summary
Loss of CLC2 and CLC3 affected endocytosis and lateral PIN3 distribution.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Loss of clathrin light chain 2 (CLC2) and CLC3e0
- Gene
- clathrin light chain 2 (CLC2) and CLC3e0
- Outcome
- endocytosis and lateral distribution of PIN3e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Loss of clathrin light chain 2 (CLC2) and CLC3 affects endocytosis and lateral distribution of PIN3”
t0“Arabidopsis”
Claim · model-written summary
Loss of CLC2 and CLC3 impaired asymmetric auxin distribution and phototropic bending triggered by blue light.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Loss of clathrin light chain 2 (CLC2) and CLC3e0
- Gene
- clathrin light chain 2 (CLC2) and CLC3e0
- Outcome
- BL-triggered establishment of asymmetric auxin distribution and consequently, phototropic bendinge0
- Direction
- impairinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Loss of clathrin light chain 2 (CLC2) and CLC3 affects endocytosis and lateral distribution of PIN3 thereby impairing BL-triggered establishment of asymmetric auxin distribution and consequently, phototropic bending”
t0“Arabidopsis”
Claim · model-written summary
Auxin efflux inhibitors affected blue light induced clathrin relocalization, endocytosis, PIN3 lateralization and auxin asymmetry.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- auxin efflux inhibitors N-1-naphthylphthalamic acid and 2,3,5-triiodobenzoic acide0
- Outcome
- BL-induced relocalization of clathrin, endocytosis and lateralization of PIN3 as well as asymmetric distribution of auxine0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“auxin efflux inhibitors N-1-naphthylphthalamic acid and 2,3,5-triiodobenzoic acid affect BL-induced relocalization of clathrin, endocytosis and lateralization of PIN3 as well as asymmetric distribution of auxin”
t0“Arabidopsis”
The same questions in other papers
light · physiology: hormones · ArabidopsisPositive and negative results
2 papers in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- positive
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