Abstract
Experimental conditions for improving the tissue culture regenerative potential and embryogenic capability of maize cultures after Agrobacterium-mediated transformation have been investigated. The parameters were investigated in five elite inbreds per se and in their crosses with A188. Embryogenic calli obtained from the inbreds spanned in a range of values from hardly responsive (11.5%) to highly responsive (79.1%) cultures. Immature embryos explanted from crosses of each inbred with A188 were all enhanced in their embryogenic and regenerative capability, with values ranging from 72.7 to 79.6%. The same genotypes, were subjected to Agrobacterium-mediated transformation, considering as target tissue either freshly explanted or five days-precultured immature embryos. Tissues were transformed with the Agrobacterium strain EHA105 with a standard binary vector based on pCAMBIA3301. The addition of 400mg/l L-cysteine in the co-cultivation step, as antioxidant to reduce callus necrosis, was also tested. Results indicated that addition of L-cys in the infection step with non pre-cultured embryos in general reduces regenerative capability, inhibiting the embryogenic development of the infected embryos. Conversely, the adoption of a pre-culture period of five days on callus induction medium, largely overcomes the inhibition. Calli treated and non-treated with L-cys exhibited comparable embryogenic potential; in addition, the regenerative capability was significantly enhanced. The protocol described is discussed as an improvement in Agrobacterium-mediated maize transformation.
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Claims 7
Claim · model-written summary
Embryogenic calli of five maize inbreds ranged from hardly responsive (11.5%) to highly responsive (79.1%)
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Agrobacterium-mediated maize transformation”
e0“investigated in five elite inbreds per se and in their crosses with A188”
e1“Embryogenic calli obtained from the inbreds spanned in a range of values from hardly responsive (11.5%) to highly responsive (79.1%) cultures”
Claim · model-written summary
Immature embryos from crosses of each inbred with A188 had 72.7 to 79.6% embryogenic and regenerative capability
- Material
- crosses of each inbred with A188e2
- Organ / stage
- Immature embryose2
- Outcome
- embryogenic and regenerative capabilitye2
- Value
- 72.7 to 79.6%e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Agrobacterium-mediated maize transformation”
e2“Immature embryos explanted from crosses of each inbred with A188 were all enhanced in their embryogenic and regenerative capability, with values ranging from 72.7 to 79.6%”
Claim · model-written summary
Adding L-cys in the infection step reduced regenerative capability of non pre-cultured maize embryos
- Organ / stage
- non pre-cultured embryose3
- Treatment
- addition of L-cys in the infection stepe3
- Outcome
- regenerative capabilitye3
- Direction
- reducese3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Agrobacterium-mediated maize transformation”
e3“addition of L-cys in the infection step with non pre-cultured embryos in general reduces regenerative capability, inhibiting the embryogenic development of the infected embryos”
Claim · model-written summary
Five-day pre-culture on callus induction medium largely overcame the L-cys inhibition of regeneration
- Treatment
- pre-culture period of five days on callus induction mediume4
- Outcome
- inhibitione4
- Direction
- overcomese4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Agrobacterium-mediated maize transformation”
e4“the adoption of a pre-culture period of five days on callus induction medium, largely overcomes the inhibition”
Claim · model-written summary
L-cys treated and non-treated calli had comparable embryogenic potential
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Agrobacterium-mediated maize transformation”
e5“Calli treated and non-treated with L-cys exhibited comparable embryogenic potential; in addition, the regenerative capability was significantly enhanced”
Claim · model-written summary
Regenerative capability of maize calli was significantly enhanced
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Agrobacterium-mediated maize transformation”
e5“Calli treated and non-treated with L-cys exhibited comparable embryogenic potential; in addition, the regenerative capability was significantly enhanced”
Claim · model-written summary
The described protocol is an improvement for Agrobacterium-mediated maize transformation
- Outcome
- an improvement in Agrobacterium-mediated maize transformatione6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Agrobacterium-mediated maize transformation”
e6“The protocol described is discussed as an improvement in Agrobacterium-mediated maize transformation”
The same questions in other papers
in vitro · disease · Zea mays (maize)Positive and negative results
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- negative
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See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.