Abstract
The effect of hydrogen peroxide (H2O2), gibberellic acid (GA3) and a short exposure of imbibed seed to 3-5 °C (prechill) on grape seed germination were investigated factorially. All three factors had some effect, either singly or in combination with one or more of the other agents, in promoting the germination of dormant seed. 2,000 ppm GA3 was the best single agent for breaking dormancy but tetrazolium staining revealed that this treatment caused the death of a small proportion of seed. Comparison of germination tests at 20, 25, 30 and 35 °C showed 25 °C tobe the best constant temperature for rapid full germination. Seed were also tested for germination in two diurnal alternating temperature regimes - 20/30 °C and 20/35 °C, the higher temperatures being applied for 8 hin each 24 h cycle. The alternating temperature of 20/30 °C proved better for testing seed for germination than either 20/35 °C or 25 °C, the best constant temperature. The following germination test procedure was devised for grape seed: a 24 h soak in 0.5 M H2O2 , a further 24 h soak in 1,000 ppm GA3, followed by a 21 d prechill at 3-5 °C with subsequent testing for germination in a diurnal alternating temperature regime of 20/30 °C with the higher temperature being applied for 8 h in each daily cycle. This procedure was tested with dormant seed of seven lots of diverse origin and found to be very effective in promoting the germination of dormant seed whilst not damaging to other seeds. In the opinion of the authors the above procedure represents a suitable practical dormancy breaking procedure for seed of Vitis spp. Entwicklung eines brauchbaren Verfahrens zur Förderung der Keimung dormanter Samen der Rebe ( Vitis spp.) Die Grenzen und Möglichkeiten der vorhandenen Verfahren zur Samenkeimung bei Reben werden diskutiert. Folgendes Verfahren zur Prüfung der Keimfähigkeit von Traubenkernen wurde ausgearbeitet: 24 h lang Einweichen in 0,5 M H2O2, weitere 24 h lang in 1000 ppm GA3, danach 21 d lang Stratifikation bei 3-5 °C, anschließend Prüfung der Keimfähigkeit unter diurnalen Wechseltemperaturen von 20/30 °C (16/8 h). Dieses Verfahren, das an den Kernen von sieben Traubensorten erprobt wurde, erwies sich als wirkungsvoll bei der Keimungsförderung dormanter Samen und schonend gegenüber den anderen Kernen.
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Claims 10
Claim · model-written summary
Hydrogen peroxide promoted germination of dormant grape seed, alone or in combination.
- Taxon
- grapee0
- Treatment
- hydrogen peroxide (H2O2)e0
- Outcome
- germination of dormant seede0
- Direction
- promotinge0
Not reportedComparator
Verbatim
e0“The effect of hydrogen peroxide (H2O2), gibberellic acid (GA3) and a short exposure of imbibed seed to 3-5 °C (prechill) on grape seed germination were investigated factorially. All three factors had some effect, either singly or in combination with one or more of the other agents, in promoting the germination of dormant seed.”
Claim · model-written summary
Gibberellic acid promoted germination of dormant grape seed, alone or in combination.
- Taxon
- grapee0
- Treatment
- gibberellic acid (GA3)e0
- Outcome
- germination of dormant seede0
- Direction
- promotinge0
Not reportedComparator
Verbatim
e0“The effect of hydrogen peroxide (H2O2), gibberellic acid (GA3) and a short exposure of imbibed seed to 3-5 °C (prechill) on grape seed germination were investigated factorially. All three factors had some effect, either singly or in combination with one or more of the other agents, in promoting the germination of dormant seed.”
Claim · model-written summary
Prechilling promoted germination of dormant grape seed, alone or in combination.
- Taxon
- grapee0
- Treatment
- a short exposure of imbibed seed to 3-5 °C (prechill)e0
- Outcome
- germination of dormant seede0
- Direction
- promotinge0
Not reportedComparator
Verbatim
e0“The effect of hydrogen peroxide (H2O2), gibberellic acid (GA3) and a short exposure of imbibed seed to 3-5 °C (prechill) on grape seed germination were investigated factorially. All three factors had some effect, either singly or in combination with one or more of the other agents, in promoting the germination of dormant seed.”
Claim · model-written summary
GA3 at 2,000 ppm was the best single dormancy-breaking agent.
Not reportedComparator
Verbatim
e0“2,000 ppm GA3 was the best single agent for breaking dormancy”
t0“grape (Vitis spp.)”
Claim · model-written summary
The 2,000 ppm GA3 treatment killed a small proportion of seed.
Not reportedComparatorDirection
Verbatim
e0“this treatment caused the death of a small proportion of seed.”
e1“2,000 ppm GA3 was the best single agent for breaking dormancy”
t0“grape (Vitis spp.)”
Claim · model-written summary
Among constant temperatures, 25 °C best supported rapid full germination.
Verbatim
e0“Comparison of germination tests at 20, 25, 30 and 35 °C showed 25 °C tobe the best constant temperature for rapid full germination.”
t0“grape (Vitis spp.)”
Claim · model-written summary
Alternating 20/30 °C was better for germination testing than 20/35 °C or constant 25 °C.
Verbatim
e0“The alternating temperature of 20/30 °C proved better for testing seed for germination than either 20/35 °C or 25 °C, the best constant temperature.”
t0“grape (Vitis spp.)”
Claim · model-written summary
The devised procedure effectively promoted dormant grape seed germination across seven lots.
Verbatim
e0“This procedure was tested with dormant seed of seven lots of diverse origin and found to be very effective in promoting the germination of dormant seed whilst not damaging to other seeds.”
e1“a 24 h soak in 0.5 M H2O2 , a further 24 h soak in 1,000 ppm GA3, followed by a 21 d prechill at 3-5 °C with subsequent testing for germination in a diurnal alternating temperature regime of 20/30 °C”
t0“grape (Vitis spp.)”
Claim · model-written summary
The devised procedure did not damage other grape seeds.
Not reportedDirection
Verbatim
e0“whilst not damaging to other seeds.”
t0“grape (Vitis spp.)”
Claim · model-written summary
The authors consider the devised procedure suitable for practical dormancy breaking in Vitis seed.
- Outcome
- suitable practical dormancy breaking proceduree0
VitisOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the opinion of the authors the above procedure represents a suitable practical dormancy breaking procedure for seed of Vitis spp.”
The same questions in other papers
gibberellin A3 · growth · VitisResults agree
2 papers in the same direction as this paper
- positive
- positive
hydrogen peroxide · growth · VitisResults agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.