Abstract
Potato cultivar Atlantic is widely grown for potato chips in the world. However, this economically important potato cultivar exhibits very poor yields and traits under severe environmental stress. To develop an efficient plant transformation system that could be used to produce large scale transgenic potato plants with enhanced tolerance to environmental stress and therefore would be beneficial for potato processing industry, Agrobacterium-mediated transformation of internodal stem explants using both superoxide dismutase (SOD) and ascorbate peroxidase (APX) genes under the control of an oxidative stress-inducible SWPA2 promoter was performed. Comparing to leaf explants, stem internodal explants were less liable to damage during manipulation, more amenable to in vitro conditions. The addition of silver thiosulfate to the selection medium considerably promoted the shoot induction from explant-derixed callus. Seven to nine shoots per stem explant were obtained. By combining the best treatments, this system yielded shoot induction frequency of 94.2% and transformation frequency of 80% of internodal stem explants. Stable integration of the transgenes was confirmed by PCR and Southern blot analyses. In conclusion, short duration (7~8 weeks), high efficiency and easy process make this system well suited for wider commercial applications of transgenic Atlantic potato plants.
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Claims 7
Claim · model-written summary
Stem internodal explants were less liable to damage during manipulation than leaf explants.
- Taxon
- potato
Solanum tuberosumt0 - Material
- Atlantice0
- Organ / stage
- stem internodal explantse1
- Treatment
- stem internodal explantse1
- Outcome
- less liable to damagee1
- Direction
- lesse1
- Comparator
- leaf explantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato Cultivar”
e0“Potato cultivar Atlantic is widely grown for potato chips in the world”
e1“Comparing to leaf explants, stem internodal explants were less liable to damage during manipulation, more amenable to in vitro conditions”
Claim · model-written summary
Silver thiosulfate in the selection medium promoted shoot induction from explant-derived callus.
- Taxon
- potatoSolanum tuberosumt0
- Material
- Atlantice0
- Treatment
- silver thiosulfatee2
- Outcome
- shoot induction from explant-derixed calluse2
- Direction
- promotede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato Cultivar”
e0“Potato cultivar Atlantic is widely grown for potato chips in the world”
e2“The addition of silver thiosulfate to the selection medium considerably promoted the shoot induction from explant-derixed callus”
Claim · model-written summary
Seven to nine shoots were obtained per stem explant.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- stem explante3
- Outcome
- shoots per stem explante3
- Value
- Seven to ninee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato Cultivar”
e3“Seven to nine shoots per stem explant were obtained”
Claim · model-written summary
Combining the best treatments gave a shoot induction frequency of 94.2% from internodal stem explants.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- internodal stem explantse4
- Outcome
- shoot induction frequencye4
- Value
- 94.2%e4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato Cultivar”
e4“By combining the best treatments, this system yielded shoot induction frequency of 94.2% and transformation frequency of 80% of internodal stem explants”
Claim · model-written summary
Combining the best treatments gave a transformation frequency of 80% for internodal stem explants.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- internodal stem explantse4
- Outcome
- transformation frequencye4
- Value
- 80%e4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato Cultivar”
e4“By combining the best treatments, this system yielded shoot induction frequency of 94.2% and transformation frequency of 80% of internodal stem explants”
Claim · model-written summary
Stable integration of the transgenes was confirmed by PCR and Southern blot analyses.
- Taxon
- potatoSolanum tuberosumt0
- Outcome
- Stable integration of the transgenese5
- Qualifier
- PCR and Southern blot analysese5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato Cultivar”
e5“Stable integration of the transgenes was confirmed by PCR and Southern blot analyses”
Claim · model-written summary
The transformation system has a short duration of 7 to 8 weeks.
- Taxon
- potatoSolanum tuberosumt0
- Material
- Atlantice6
- Outcome
- duratione6
- Value
- 7~8 weekse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato Cultivar”
e6“In conclusion, short duration (7~8 weeks), high efficiency and easy process make this system well suited for wider commercial applications of transgenic Atlantic potato plants”
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See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.