Abstract
Twenty two Bacillus spp. isolates from the Phaseolus vulgaris rhizosphere were identified using Biolog™, GC-FAME and 23S rDNA analysis. Some Bacillus isolates produced copious amounts of indoleacetic acid in vitro, in culture medium supplemented with L-tryptophan. Spontaneous cc-methyl tryptophan resistant mutants of Bacillus megaterium S49 exhibited altered IAA production and excreted tryptophan into the culture medium. Coinoculation of Rhizobium etli TAL 182 with some Bacillus spp. under gnotobiotic conditions promoted nodulation of P. vulgaris. In contrast, decreased nodulation was observed in response to low and high lAA-producing Bacillus mutants, suggesting that the nodulation response in bean was not dependent on the in vitro level of IAA production by the Bacillus coinoculant. Coinoculation of Bacillus spp. and Rhizobium spp. resulted in a unique and significant synergistic enhancement of lateral root formation and root hair initiation which was independent of plant host. These observations provided direct evidence for a beneficial interaction on root morphology which may account for enhanced nodulation by creating additional infection sites for the Rhizobium. The presence of live Rhizobium and Bacillus was necessary for the root hair proliferation response. Coinoculation of Rhizobium TAL 182 with cell extracts of Bacillus S49 failed to induce root hair proliferation or enhance nodulation of P. vulgaris. A biologically active Rhizobium TAL 182 was essential for the observed response, indicating that root hair proliferation and enhanced nodulation were direct consequences of the bacterial species interaction. Nodulation and symbiotic specificity of Rhizobium TAL 182 were altered by the presence of Bacillus S49. Split-root experiments showed that coinoculation suppressed host-controlled regulation of nodulation. Bacillus S49 facilitated heterologous nodulation of Rhizobium TAL 182 on P. acutifolius, indicating that a host plant response was also involved in the bacterial interaction. Exogenous aminoethoxy vinyl glycine, a negative regulator of ethylene biosynthesis, inhibited root hair formation in P. vulgaris. These inhibitory effects were reversed by the presence of both Bacillus S49 and Rhizobium TAL 182. The interaction between bacterial species on root hair induction and nodulation patterns in P. vulgaris is discussed.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 11
Claim · model-written summary
Coinoculation of Rhizobium etli TAL 182 with some Bacillus spp. promoted nodulation of P. vulgaris under gnotobiotic conditions.
- Taxon
- P. vulgaris
Phaseolus vulgarise0 - Treatment
- Coinoculation of Rhizobium etli TAL 182 with some Bacillus spp.e0
- Interactor
- Rhizobium etli TAL 182Rhizobium etlie0symbiont
- Second interactor
- Bacillus spp.e0symbiont
- Outcome
- nodulatione0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Coinoculation of Rhizobium etli TAL 182 with some Bacillus spp. under gnotobiotic conditions promoted nodulation of P. vulgaris.”
Claim · model-written summary
Low and high IAA-producing Bacillus mutants decreased bean nodulation.
- Taxon
- Phaseolus vulgaris L.Phaseolus vulgarist0
- Treatment
- low and high lAA-producing Bacillus mutantse0
- Interactor
- Bacillus mutantse0symbiont
- Outcome
- nodulatione0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“decreased nodulation was observed in response to low and high lAA-producing Bacillus mutants”
t0“Changes in root hair morphogenesis and nodulation of Phaseolus vulgaris L. in the presence of bacillus and rhizobium”
Claim · model-written summary
Bacillus and Rhizobium coinoculation significantly enhanced lateral root formation and root hair initiation.
- Taxon
- Phaseolus vulgaris L.Phaseolus vulgarist0
- Treatment
- Coinoculation of Bacillus spp. and Rhizobium spp.e0
- Interactor
- Bacillus spp.e0symbiont
- Outcome
- lateral root formation and root hair initiatione0
- Direction
- enhancemente0
- Qualifier
- independent of plant hoste0
RhizobiumNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Coinoculation of Bacillus spp. and Rhizobium spp. resulted in a unique and significant synergistic enhancement of lateral root formation and root hair initiation which was independent of plant host.”
t0“Changes in root hair morphogenesis and nodulation of Phaseolus vulgaris L. in the presence of bacillus and rhizobium”
Claim · model-written summary
Living Rhizobium and Bacillus were necessary for root hair proliferation.
- Taxon
- Phaseolus vulgaris L.Phaseolus vulgarist0
- Second interactor
- Bacilluse0symbiont
- Outcome
- root hair proliferation responsee0
- Qualifier
- presence of live Rhizobium and Bacilluse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The presence of live Rhizobium and Bacillus was necessary for the root hair proliferation response.”
t0“Changes in root hair morphogenesis and nodulation of Phaseolus vulgaris L. in the presence of bacillus and rhizobium”
Claim · model-written summary
Rhizobium TAL 182 with Bacillus S49 cell extracts did not induce P. vulgaris root hair proliferation.
- Taxon
- P. vulgarisPhaseolus vulgarise0
- Treatment
- Coinoculation of Rhizobium TAL 182 with cell extracts of Bacillus S49e0
- Interactor
- Rhizobium TAL 182e0symbiont
- Second interactor
- Bacillus S49e0symbiont
- Outcome
- root hair proliferatione0
- Direction
- failed to inducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Coinoculation of Rhizobium TAL 182 with cell extracts of Bacillus S49 failed to induce root hair proliferation or enhance nodulation of P. vulgaris.”
Claim · model-written summary
Rhizobium TAL 182 with Bacillus S49 cell extracts did not enhance P. vulgaris nodulation.
- Taxon
- P. vulgarisPhaseolus vulgarise0
- Treatment
- Coinoculation of Rhizobium TAL 182 with cell extracts of Bacillus S49e0
- Interactor
- Rhizobium TAL 182e0symbiont
- Second interactor
- Bacillus S49e0symbiont
- Outcome
- nodulatione0
- Direction
- failed to induce root hair proliferation or enhancee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Coinoculation of Rhizobium TAL 182 with cell extracts of Bacillus S49 failed to induce root hair proliferation or enhance nodulation of P. vulgaris.”
Claim · model-written summary
Bacillus S49 altered Rhizobium TAL 182 nodulation and symbiotic specificity.
- Taxon
- Phaseolus vulgaris L.Phaseolus vulgarist0
- Treatment
- presence of Bacillus S49e0
- Interactor
- Rhizobium TAL 182e0symbiont
- Second interactor
- Bacillus S49e0symbiont
- Outcome
- Nodulation and symbiotic specificity of Rhizobium TAL 182e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nodulation and symbiotic specificity of Rhizobium TAL 182 were altered by the presence of Bacillus S49.”
t0“Changes in root hair morphogenesis and nodulation of Phaseolus vulgaris L. in the presence of bacillus and rhizobium”
Claim · model-written summary
Coinoculation suppressed host-controlled regulation of nodulation in split-root experiments.
- Taxon
- Phaseolus vulgaris L.Phaseolus vulgarist0
- Treatment
- coinoculatione0
- Outcome
- host-controlled regulation of nodulatione0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Split-root experiments showed that coinoculation suppressed host-controlled regulation of nodulation.”
t0“Changes in root hair morphogenesis and nodulation of Phaseolus vulgaris L. in the presence of bacillus and rhizobium”
Claim · model-written summary
Bacillus S49 facilitated heterologous nodulation of Rhizobium TAL 182 on P. acutifolius.
- Taxon
- P. acutifolius
Phaseolus acutifoliuse0 - Treatment
- Bacillus S49e0
- Interactor
- Rhizobium TAL 182e0symbiont
- Second interactor
- Bacillus S49e0symbiont
- Outcome
- heterologous nodulation of Rhizobium TAL 182e0
- Direction
- facilitatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Bacillus S49 facilitated heterologous nodulation of Rhizobium TAL 182 on P. acutifolius”
Claim · model-written summary
Exogenous aminoethoxy vinyl glycine inhibited root hair formation in P. vulgaris.
- Taxon
- P. vulgarisPhaseolus vulgarise0
- Treatment
- Exogenous aminoethoxy vinyl glycinee0
- Outcome
- root hair formatione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous aminoethoxy vinyl glycine, a negative regulator of ethylene biosynthesis, inhibited root hair formation in P. vulgaris.”
Claim · model-written summary
Bacillus S49 and Rhizobium TAL 182 reversed the inhibitory effect on root hair formation.
- Taxon
- Phaseolus vulgaris L.Phaseolus vulgarist0
- Treatment
- presence of both Bacillus S49 and Rhizobium TAL 182e0
- Interactor
- Bacillus S49e0symbiont
- Second interactor
- Rhizobium TAL 182e0symbiont
- Outcome
- inhibitory effectse0
- Direction
- reversede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These inhibitory effects were reversed by the presence of both Bacillus S49 and Rhizobium TAL 182.”
t0“Changes in root hair morphogenesis and nodulation of Phaseolus vulgaris L. in the presence of bacillus and rhizobium”
The same questions in other papers
Rhizobium etli · symbiosis · Phaseolus vulgaris (common bean)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
Rhizobium · symbiosis · Phaseolus vulgaris (common bean)Positive and negative results
1 paper in the same direction as this paper · 16 in the opposite direction
- positive
- positive
- positive
14 papers more in this group
See all 18 papers →Rhizobium · growth · Phaseolus vulgaris (common bean)Positive and negative results
21 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
20 papers more in this group
See all 24 papers →inoculation · growth · Phaseolus vulgaris (common bean)Results differ
105 papers in the same direction as this paper · 20 in the opposite direction
- negative
- negative
- negative
122 papers more in this group
See all 126 papers →botanical · symbiosis · Phaseolus vulgaris (common bean)Positive and negative results
1 paper in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →botanical · growth · Phaseolus vulgaris (common bean)Positive and negative results
37 papers in the same direction as this paper · 50 in the opposite direction
- positive
- positive
- positive
84 papers more in this group
See all 88 papers →inoculation · symbiosis · Phaseolus vulgaris (common bean)Positive and negative results
82 papers in the same direction as this paper · 7 in the opposite direction
- no effect
- no effect
- negative
86 papers more in this group
See all 90 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.