Abstract
Although necrotrophic pathogens cause many devastating plant diseases, our understanding of the plant defense response to them is limited. Here, we found that loss of function of WRKY57 enhanced the resistance of Arabidopsis (Arabidopsis thaliana) against Botrytis cinerea infection. Further investigation suggested that the negative regulation of WRKY57 against B cinerea depends on the jasmonic acid (JA) signaling pathway. Chromatin immunoprecipitation experiments revealed that WRKY57 directly binds to the promoters of JASMONATE ZIM-DOMAIN1 (JAZ1) and JAZ5, encoding two important repressors of the JA signaling pathway, and activates their transcription. In vivo and in vitro experiments demonstrated that WRKY57 interacts with nuclear-encoded SIGMA FACTOR BINDING PROTEIN1 (SIB1) and SIB2. Further experiments display that the same domain, the VQ motif, of SIB1 and SIB2 interact with WRKY33 and WRKY57. Moreover, transient transcriptional activity assays confirmed that WRKY57 and WRKY33 competitively regulate JAZ1 and JAZ5, SIB1 and SIB2 further enhance these competitions of WRKY57 to WRKY33. Therefore, coordinated regulation of Arabidopsis against B cinerea by transcription activators and repressors would benefit plants by allowing fine regulation of defense.
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Claims 8
Claim · model-written summary
Loss of function of WRKY57 enhanced Arabidopsis resistance to Botrytis cinerea.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae1 - Treatment
- loss of function of WRKY57e1
- Interactor
- Botrytis cinerea
Botrytis cinereae1pathogen - Gene
- WRKY57e1
- Outcome
- resistancee1
- Direction
- enhancede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“loss of function of WRKY57 enhanced the resistance of Arabidopsis (Arabidopsis thaliana) against Botrytis cinerea infection”
Claim · model-written summary
The negative regulation of WRKY57 against Botrytis cinerea depends on the jasmonic acid signalling pathway.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Interactor
- B cinereaBotrytis cinereae2pathogen
- Gene
- WRKY57e2
- Outcome
- negative regulation of WRKY57 against B cinereae2
- Qualifier
- depends on the jasmonic acid (JA) signaling pathwaye2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“loss of function of WRKY57 enhanced the resistance of Arabidopsis (Arabidopsis thaliana) against Botrytis cinerea infection”
e2“the negative regulation of WRKY57 against B cinerea depends on the jasmonic acid (JA) signaling pathway”
Claim · model-written summary
WRKY57 directly binds the promoters of JAZ1 and JAZ5 and activates their transcription.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- JASMONATE ZIM-DOMAIN1 (JAZ1) and JAZ5e3
- Outcome
- activates their transcriptione3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“loss of function of WRKY57 enhanced the resistance of Arabidopsis (Arabidopsis thaliana) against Botrytis cinerea infection”
e3“WRKY57 directly binds to the promoters of JASMONATE ZIM-DOMAIN1 (JAZ1) and JAZ5, encoding two important repressors of the JA signaling pathway, and activates their transcription”
Claim · model-written summary
WRKY57 interacts with SIB1 and SIB2 in vivo and in vitro.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- SIGMA FACTOR BINDING PROTEIN1 (SIB1) and SIB2e4
- Outcome
- interacts withe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“loss of function of WRKY57 enhanced the resistance of Arabidopsis (Arabidopsis thaliana) against Botrytis cinerea infection”
e4“In vivo and in vitro experiments demonstrated that WRKY57 interacts with nuclear-encoded SIGMA FACTOR BINDING PROTEIN1 (SIB1) and SIB2”
Claim · model-written summary
The VQ motif of SIB1 and SIB2 interacts with WRKY33 and WRKY57.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- SIB1 and SIB2e5
- Outcome
- interact with WRKY33 and WRKY57e5
- Qualifier
- the VQ motife5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“loss of function of WRKY57 enhanced the resistance of Arabidopsis (Arabidopsis thaliana) against Botrytis cinerea infection”
e5“Further experiments display that the same domain, the VQ motif, of SIB1 and SIB2 interact with WRKY33 and WRKY57”
Claim · model-written summary
WRKY57 and WRKY33 competitively regulate JAZ1 and JAZ5.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- JAZ1 and JAZ5e6
- Outcome
- competitively regulatee6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“loss of function of WRKY57 enhanced the resistance of Arabidopsis (Arabidopsis thaliana) against Botrytis cinerea infection”
e6“transient transcriptional activity assays confirmed that WRKY57 and WRKY33 competitively regulate JAZ1 and JAZ5”
Claim · model-written summary
SIB1 and SIB2 further enhance the competition of WRKY57 with WRKY33.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- SIB1 and SIB2e7
- Outcome
- further enhance these competitions of WRKY57 to WRKY33e7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“loss of function of WRKY57 enhanced the resistance of Arabidopsis (Arabidopsis thaliana) against Botrytis cinerea infection”
e7“SIB1 and SIB2 further enhance these competitions of WRKY57 to WRKY33”
Claim · model-written summary
Coordinated regulation of Arabidopsis against B. cinerea by activators and repressors would benefit plants by allowing fine regulation of defense.
- Taxon
- ArabidopsisArabidopsise0
- Interactor
- B cinereaBotrytis cinereae0pathogen
- Outcome
- fine regulation of defensee0
- Qualifier
- by transcription activators and repressorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“coordinated regulation of Arabidopsis against B cinerea by transcription activators and repressors would benefit plants by allowing fine regulation of defense”
The same questions in other papers
loss of function · Botrytis cinerea · Arabidopsis thaliana (thale cress)Positive and negative results
2 papers in the same direction as this paper · 4 in the opposite direction
- negative
- negative
- negative
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.